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hosseini, S., Homayuoni rad, A. (2023). Evaluating the Antimicrobial Effect of Postbiotic Extract From Lactobacillus Casei on E.coli, S.aureus, P.nutatum, and C.albicans by Using the Well Diffusion Agar Method. , 22(1), 81-96. doi: 10.22092/fooder.2023.357471.1326
samira hosseini; Aziz Homayuoni rad. "Evaluating the Antimicrobial Effect of Postbiotic Extract From Lactobacillus Casei on E.coli, S.aureus, P.nutatum, and C.albicans by Using the Well Diffusion Agar Method". , 22, 1, 2023, 81-96. doi: 10.22092/fooder.2023.357471.1326
hosseini, S., Homayuoni rad, A. (2023). 'Evaluating the Antimicrobial Effect of Postbiotic Extract From Lactobacillus Casei on E.coli, S.aureus, P.nutatum, and C.albicans by Using the Well Diffusion Agar Method', , 22(1), pp. 81-96. doi: 10.22092/fooder.2023.357471.1326
hosseini, S., Homayuoni rad, A. Evaluating the Antimicrobial Effect of Postbiotic Extract From Lactobacillus Casei on E.coli, S.aureus, P.nutatum, and C.albicans by Using the Well Diffusion Agar Method. , 2023; 22(1): 81-96. doi: 10.22092/fooder.2023.357471.1326

Evaluating the Antimicrobial Effect of Postbiotic Extract From Lactobacillus Casei on E.coli, S.aureus, P.nutatum, and C.albicans by Using the Well Diffusion Agar Method

تحقیقات مهندسی صنایع غذایی
Volume 22, Issue 1 - Serial Number 74, June 2023, Pages 81-96    PDF (1.05 M)
Document Type: Research Paper
DOI: 10.22092/fooder.2023.357471.1326
Authors
samira hosseini* 1; Aziz Homayuoni rad2
1, Faculty of Nutrition and Food Sciences, Tabriz University of Medical Science, Tabriz, Iran
2Professor Department of Food Science and Technology, Faculty of Nutrition and Food Sciences, Tabriz University of Medical Science, Tabriz, Iran
Abstract
Humans are always looking for a way to preserve food from chemical, microbial, and food pathogen spoilage. The use of probiotics is facing challenges today. To get out of this impasse, probiotics can be converted into postbiotics and used in the treatment of various diseases. postbiotics provide a significant source of energy for colon epithelial cells and play a role in their growth and differentiation. To evaluate the antimicrobial effect, the postbiotic and was evaluated against the pathogens Escherichia. coli, Staphylococcus aureus, Penicillium notatum, and Candida albicans by Well Diffusion Agar method in measuring the diameter of the halo of non-growth. Skewness and kurtosis indices were used to check the normality of the data. In evaluating the antimicrobial effect of the postbiotic extract by diffusion method in the well and measuring, the largest diameter was formed in the concentration of 800 µl/ml in E. coli and Staphylococcus aureus bacteria. in Penicillium notatum and Candida albicans, a non-growth halo was observed after three repetitions. According to the results obtained from the present research, it can be concluded that Lactobacillus casei is one of the bacteria, whose postbiotics can have an inhibitory effect against pathogenic bacteria such as E. coli and Staphylococcus aureus. The results obtained in the measurements of the diameter of the non-growth halo of the evaluated pathogens showed a difference in the effect of the postbiotic extract.
Keywords
Lactobacillus casei; probiotics; postbiotics; pathogenic pathogens; Well Diffusion Agar method
References

Abdelazez, A., H. Abdelmotaal, S. E. Evivie, M. Bikheet, R. Sami, H. Mohamed and X. Meng. 2022. Verification of Lactobacillus brevis tolerance to simulated gastric juice and the potential effects of postbiotic gamma-aminobutyric acid in streptozotocin-induced diabetic mice. Food Science and Human Wellness. 11(1): 165-176.

Abouloifa, H., S. Gaamouche, Y. Rokni, I. Hasnaoui, R. Bellaouchi, N. Ghabbour, S. Karboune, M. Brasca, G. D'Hallewin and R. B. Salah. 2021. Antifungal activity of probiotic Lactobacillus strains isolated from natural fermented green olives and their application as food bio-preservative. Biological Control. 152, 104450.

Balaguer, F., M. Enrique, S. Llopis, M. Barrena, V. Navarro, B. Álvarez, E. Chenoll, D. Ramon, M. Tortajada and P. Martorell. 2022. Lipoteichoic acid from Bifidobacterium animalis subsp. lactis BPL1: a novel postbiotic that reduces fat deposition via IGF‐1 pathway. Microbial biotechnology. 15(3): 805-816.

Barros, C. P., J. T. Guimaraes, E. A. Esmerino, M. C. K. Duarte, M. C. Silva, R. Silva, B. M. Ferreira, A. S. Sant’Ana, M. Q. Freitas and A. G. Cruz. 2020. Paraprobiotics and postbiotics: concepts and potential applications in dairy products. Current Opinion in Food Science. 32, 1-8.

Beikzadeh, S., S. M. Hosseini, V. Mofid, S. Ramezani, M. Ghorbani, A. Ehsani and A. M. Mortazavian. 2021. Electrospun ethyl cellulose/poly caprolactone/gelatin nanofibers: The investigation of mechanical, antioxidant, and antifungal properties for food packaging. International Journal of Biological Macromolecules. 191, 457-464.

Binda, S., C. Hill, E. Johansen, D. Obis, B. Pot, M. E. Sanders, A. Tremblay and A. C. Ouwehand. 2020. Criteria to qualify microorganisms as “probiotic” in foods and dietary supplements. Frontiers in Microbiology. 1662.

Butera, A., S. Gallo, M. Pascadopoli, D. Taccardi and A. Scribante. 2022. Home oral care of periodontal patients using antimicrobial gel with postbiotics, lactoferrin, and aloe barbadensis leaf juice powder vs. conventional chlorhexidine gel: A split-mouth randomized clinical trial. Antibiotics. 11(1): 118.

Chen, H., H. Ju, Y. Wang, G. Du, X. Yan, Y. Cui, Y. Yuan and T. Yue. 2021. Antifungal activity and mode of action of lactic acid bacteria isolated from kefir against Penicillium expansum. Food Control. 130, 108274.

Cuevas-González, P., A. Liceaga and J. Aguilar-Toalá. 2020. Postbiotics and paraprobiotics: From concepts to applications. Food research international. 136, 109502.

Datta, P., L. Fu, P. Brodfuerer, J. S. Dordick and R. J. Linhardt. 2021. High density fermentation of probiotic E. coli Nissle 1917 towards heparosan production, characterization, and modification. Applied Microbiology and Biotechnology. 105(3): 1051-1062.

DeFilipp, Z., P. P. Bloom, M. Torres Soto, M. K. Mansour, M. R. Sater, M. H. Huntley, S. Turbett, R. T. Chung, Y.-B. Chen and E. L. Hohmann. 2019. Drug-resistant E. coli bacteremia transmitted by fecal microbiota transplant. New England Journal of Medicine. 381(21): 2043-2050.

Dunand, E., P. Burns, A. Binetti, C. Bergamini, G. H. Peralta, L. Forzani, J. Reinheimer and G. Vinderola. 2019. Postbiotics produced at laboratory and industrial level as potential functional food ingredients with the capacity to protect mice against Salmonella infection. Journal of applied microbiology. 127(1): 219-229.

Ebrahimi, M., A. Sadeghi, D. Rahimi, H. Purabdolah and S. Shahryari. 2021. Postbiotic and anti-aflatoxigenic capabilities of Lactobacillus kunkeei as the potential probiotic LAB isolated from the natural honey. Probiotics and Antimicrobial Proteins. 13(2): 343-355.

El-Mokhtar, M. A., K. M. Hassanein, A. S. Ahmed, G. F. Gad, M. M. Amin and O. F. Hassanein. 2020. Antagonistic activities of cell-free supernatants of lactobacilli against extended-spectrum β-lactamase producing Klebsiella pneumoniae and Pseudomonas aeruginosa. Infection and Drug Resistance. 13: 543.

Gajdács, M. 2019. The continuing threat of methicillin-resistant Staphylococcus aureus. Antibiotics. 8(2): 52.

Gharibzahedi, S. M. T. and B. Smith. 2021. Legume proteins are smart carriers to encapsulate hydrophilic and hydrophobic bioactive compounds and probiotic bacteria: A review. Comprehensive Reviews in Food Science and Food Safety. 20(2): 1250-1279.

Hassan, B. A., M. A. Jebor and Z. M. Ali. 2018. Purification and Characterization of the Glucose Oxidase from Penicillium notatum. International Journal of Pharmaceutical Quality Assurance. 9(1): 55-63.

He, L., H. Yang, J. Tang, Z. Liu, Y. Chen, B. Lu, H. He, S. Tang, Y. Sun and F. Liu. 2019. Intestinal probiotics E. coli Nissle 1917 as a targeted vehicle for delivery of p53 and Tum-5 to solid tumors for cancer therapy. Journal of biological engineering. 13(1): 1-13.

Husby, S., S. Koletzko, I. Korponay-Szabó, K. Kurppa, M. L. Mearin, C. Ribes-Koninckx, R. Shamir, R. Troncone, R. Auricchio and G. Castillejo. 2020. European Society Paediatric Gastroenterology, Hepatology and Nutrition guidelines for diagnosing coeliac disease 2020. Journal of pediatric gastroenterology and nutrition. 70(1): 141-156.

Johnson, C. N., M. H. Kogut, K. Genovese, H. He, S. Kazemi and R. J. Arsenault. 2019. Administration of a postbiotic causes immunomodulatory responses in broiler gut and reduces disease pathogenesis following challenge. Microorganisms. 7(8): 268.

Kaur, S. and R. Kaur. 2019. Biosurfactant from Lactobacillus sp. as an antibiofilm agent. BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology.100(3).

Kazemi, A., A. A. Noorbala, K. Azam, M. H. Eskandari and K. Djafarian. 2019. Effect of probiotic and prebiotic vs placebo on psychological outcomes in patients with major depressive disorder: A randomized clinical trial. Clinical Nutrition. 38(2): 522-528.

Khalesi, S., N. Bellissimo, C. Vandelanotte, S. Williams, D. Stanley and C. Irwin. 2019. A review of probiotic supplementation in healthy adults: helpful or hype? European journal of clinical nutrition. 73(1): 24-37.

Kim, H. and S.-S. Kang. 2019. Antifungal activities against Candida albicans, of cell-free supernatants obtained from probiotic Pediococcus acidilactici HW01. Archives of oral biology. 99: 113-119.

Kostelac, D., M. Gerić, G. Gajski, K. Markov, A. M. Domijan, I. Čanak, Ž. Jakopović, I. K. Svetec, B. Žunar and J. Frece. 2021. Lactic acid bacteria isolated from equid milk and their extracellular metabolites show great probiotic properties and anti-inflammatory potential. International Dairy Journal. 112: 104828.

Layus, B. I., C. L. Gerez and A. V. Rodriguez. 2020. Antibacterial activity of Lactobacillus plantarum CRL 759 against methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa. Arabian Journal for Science and Engineering. 45(6): 4503-4510.

Leo, A., C. De Caro, P. Mainardi, M. Tallarico, V. Nesci, N. Marascio, P. Striano, E. Russo, A. Constanti and G. De Sarro. 2021. Increased efficacy of combining prebiotic and postbiotic in mouse models relevant to autism and depression. Neuropharmacology 198: 108782. Culture supernatants of Lactobacillus gasseri and L. crispatus inhibit Candida albicans biofilm formation and adhesion to HeLa cells. Mycopathologia. 183(4): 691-700.

Morales-Ferré, C., I. Azagra-Boronat, M. Massot-Cladera, S. Tims, K. Knipping, J. Garssen, J. Knol, À. Franch, M. Castell and M. J. Rodríguez-Lagunas. 2021. Effects of a postbiotic and prebiotic mixture on suckling rats’ microbiota and immunity. Nutrients. 13(9): 2975.

Nataraj, B. H., S. A. Ali, P. V. Behare and H. Yadav. 2020. Postbiotics-parabiotics: The new horizons in microbial biotherapy and functional foods. Microbial cell factories. 19(1): 1-22.

Nataraj, B. H. and R. H. Mallappa. 2021. Antibiotic resistance crisis: an update on antagonistic interactions between probiotics and methicillin-resistant Staphylococcus aureus (MRSA). Current Microbiology. 78(6): 2194-2211.

Osman, A., N. El-Gazzar, T. N. Almanaa, A. El-Hadary and M. Sitohy. 2021. Lipolytic postbiotic from Lactobacillus paracasei manages metabolic syndrome in albino wistar rats. Molecules. 26(2): 472.

Oyewole, O. A., S. S. L.-T. Zobeashia, E. O. Oladoja, R. O. Raji, E. E. Odiniya and A. M. Musa.2019. Biosorption of heavy metal polluted soil using bacteria and fungi isolated from soil. SN Applied Sciences. 1(8): 1-8.

Purton, T., L. Staskova, M. M. Lane, S. L. Dawson, M. West, J. Firth, G. Clarke, J. F. Cryan, M. Berk and A. O’Neil. 2021. Prebiotic and probiotic supplementation and the tryptophan-kynurenine pathway: A systematic review and meta analysis. Neuroscience & Biobehavioral Reviews. 123: 1-13.

Rad, A. H., S. Hosseini and H. Pourjafar. 2022. Postbiotics as dynamic biological molecules for antimicrobial activity: A mini-review. Biointerface Research in Applied Chemistry. 12(5): 6543-6556.

Sabbatini, S., S. Visconti, M. Gentili, E. Lusenti, E. Nunzi, S. Ronchetti, S. Perito, R. Gaziano and C. Monari. 2021. Lactobacillus iners Cell-Free Supernatant Enhances Biofilm Formation and Hyphal/Pseudohyphal Growth by Candida albicans Vaginal Isolates. Microorganisms. 9(12): 2577.

Samira, H., H.-R. Aziz, K. Hossein Samadi and D. Nazli. 2022. Evaluating the antimicrobial effect of postbiotic extract from Lactobacillus casei on Escherichia coli in commercial sterilized milk. Journal of Food Safety and Hygiene. 8(1).

Sudan, S., R. Flick, L. Nong and J. Li. 2021. Potential Probiotic Bacillus subtilis Isolated from a Novel Niche Exhibits Broad Range Antibacterial Activity and Causes Virulence and Metabolic Dysregulation in Enterotoxic E. coli. Microorganisms. 9(7): 1483.

Toushik, S. H., J.-H. Park, K. Kim, M. Ashrafudoulla, M. S. I. Ulrich, M. F. R. Mizan, P. K. Roy, W.-B. Shim, Y.-M. Kim and S. H. Park. 2022. Antibiofilm efficacy of Leuconostoc mesenteroides J. 27-derived postbiotic and food-grade essential oils against Vibrio parahaemolyticus, Pseudomonas aeruginosa, and Escherichia coli alone and in combination, and their application as a green preservative in the seafood industry. Food Research International. 156: 111163.

Turner, N. A., B. K. Sharma-Kuinkel, S. A. Maskarinec, E. M. Eichenberger, P. P. Shah, M. Carugati, T. L. Holland and V. G. Fowler. 2019. Methicillin-resistant Staphylococcus aureus: an overview of basic and clinical research. Nature Reviews Microbiology. 17(4): 203-218.

Warda, A. K., A. G. Clooney, F. Ryan, P. H. de Almeida Bettio, G. Di Benedetto, R. P. Ross and C. Hill. 2021. A postbiotic consisting of heat-treated lactobacilli has a bifidogenic effect in pure culture and in human fermented fecal communities. Applied and environmental microbiology. 87(8): e02459-02420.

Wirawati, C. U., D. E. Nirmagustina and Y. R. Widodo. 2020. Antihypertensive peptides produced by indigenous lactic acid bacteria from dadih origin. Pakistan Journal of Biotechnology. 17(2): 85-91.

Zendeboodi, F., N. Khorshidian, A. M. Mortazavian and A. G. da Cruz. 2020. Probiotic: conceptualization from a new approach. Current Opinion in Food Science. 32: 103-123.

Żółkiewicz, J., A. Marzec, M. Ruszczyński and W. Feleszko. 2020. Postbiotics—a step beyond pre-and probiotics. Nutrients. 12(8): 2189.

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