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اثر مقادیر مختلف نیکل بر برخی پارامترهای فیزیولوژیکی و سنتز ترکیبهای ثانویه در گیاه بنگدانه (Hyoscyamus niger L.) | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 7، دوره 30، شماره 3 - شماره پیاپی 65، مرداد 1393، صفحه 402-413 اصل مقاله (296.65 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2014.7674 | ||
| نویسندگان | ||
| طیبه حیدری1؛ زهرا اسرار2؛ فاطمه نصیبی* 3 | ||
| 1دانشجوی کارشناسیارشد، گروه زیستشناسی، دانشگاه شهید باهنر کرمان | ||
| 2دانشیار، گروه زیستشناسی، دانشگاه شهید باهنر کرمان | ||
| 3استادیار، گروه زیستشناسی، دانشگاه شهید باهنر کرمان | ||
| چکیده | ||
| یکی از روشهای افزایش ترکیبهای ثانویه در گیاهان استفاده از محرکهای غیرزیستی است. در این بررسی اثر فلز سنگین نیکل بهعنوان یک محرک غیرزیستی بر برخی پارامترهای فیزیولوژیکی گیاه بنگدانه (Hyoscyamus niger L.) مورد مطالعه قرار گرفت تا امکان استفاده از غلظتهای غیرتنشی آن در مطالعات بعدی برای افزایش ترکیبهای ثانویه مورد آزمایش قرار گیرد. این پژوهش در آزمایشگاه تحقیقاتی گروه زیستشناسی دانشگاه شهید باهنر کرمان در قالب یک طرح کاملاً تصادفی با سه تیمار و 3 تکرار انجام شد. در این بررسی بذرهای گیاه بنگدانه در ژرمیناتور و با اعمال تیمار ژیبرلین جوانه زدند و بعد به گلدانهای حاوی پیتماس و شن منتقل شدند. پس از 40 روز رشد، گیاهان تحت تأثیر غلظتهای 50 و 100 میکرومولار نیکل در شرایط آبکشت قرار گرفتند (مقادیر نیکل به محلول غذایی اضافه گردید). در این بررسی رنگیزههای فتوسنتزی تحت تأثیر نیکل کاهش معنیداری را نشان دادند. اما مقدار پراکسید هیدروژن، پراکسیداسیون لیپید و فعالیت آنزیمهای کاتالاز، گایاکول پراکسیداز و آسکوربات پراکسیداز افزایش یافت. بررسیهای انجام شده بر روی ماکرومولکولهای اصلی گیاه مانند پروتئینها، لیپیدهای غشایی و کلروفیل نشان داد که اثر غلظتهای 50 و 100 میکرومولار نیکل در ایجاد تنش بر این مولکولها تقریباً مشابه است، اما فعالیت آنزیم فنیلآلانین آمونیالیاز و سنتز ترکیبهای پلیفنلی از پاسخهای بعدی گیاه هستند که در غلظت 100 میکرومولار نیکل مشاهده میشوند. بنابراین به نظر میرسد که غلظتهای بالاتر نیکل (100 میکرومولار) برای افزایش ترکیبهای ثانویه در این گیاه مؤثرتر بوده است. | ||
| کلیدواژهها | ||
| آسکوربات پراکسیداز؛ ترکیبهای فنلی؛ تنش اکسیداتیو؛ رنگیزههای فتوسنتزی؛ فنیلآلانین آمونیالیاز | ||
| عنوان مقاله [English] | ||
| Effect of different nickel amounts on some physiological parameters and secondary metabolite synthesis in Hyoscyamus niger L. | ||
| نویسندگان [English] | ||
| T. Heidari1؛ Z. Asrar2؛ F. Nasibi3 | ||
| 1MSc. Student, Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran | ||
| 2Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran | ||
| 3Biology Department, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran | ||
| چکیده [English] | ||
| One of the methods employed to enhance plant secondary metabolite production is the use of abiotic elicitors in plants. In this research, the effect of heavy metal nickel as abiotic elicitors was studied on some physiological parameters in Hyoscyamus niger L. to investigate the use of unstressful concentration of this metal in order to increase secondary metabolite synthesis in later researches. This research was carried out in the Department of Biology of Shahid Bahonar University of Kerman in a complete randomized design with three treatments and three replicates. The seeds of Hyoscyamus germinated in germinator under gibberellin treatment and then were transfered to the pots of sand and peat. After 40 days of growth, the plants were treated with 50 and 100 µM nickel in hydroponics condition (nickel amounts were added to nutrient solution). According to the obtained results, photosynthetic pigments reduced under Ni treatment while hydrogen peroxide, lipid peroxidation and antioxidant enzymes activity increased. Investigation of Ni stress on main macromolecules such as protein, membrane lipids and chlorophyll showed that the effects of 50 and 100 µM Ni was same, while the PAL activity and polyphenols synthesis were the second responses of plants to stress obserevd in 100 µM concentrations. Therefore, it seems that the higher concentration of Ni (100 µM) was more effective for the increment of secondary metabolite in this plant. | ||
| کلیدواژهها [English] | ||
| ascorbate peroxidase, Phenolic compound, oxidative stress, photosynthetic pigments, phenylalanine ammonia lyase | ||
| مراجع | ||
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