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اثر متیلجاسمونات بر آنزیمهای متابولیسمی و مواد فنلی در گیاه دارویی آگاستاکه (Agastache foeniculum [Pursh] Kuntze) | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 3، دوره 30، شماره 3 - شماره پیاپی 65، مرداد 1393، صفحه 361-369 اصل مقاله (302.71 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2014.7500 | ||
| نویسندگان | ||
| فاطمه رؤففرد1؛ مظفر شریفی* 2؛ رضا امیدبیگی3؛ فاطمه سفیدکن4؛ مهرداد بهمنش5؛ نوراله احمدی6 | ||
| 1دانشجوی دکترا، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه تربیت مدرس | ||
| 2دانشیار، گروه علوم گیاهی، دانشکده علوم زیستی، دانشگاه تربیت مدرس | ||
| 3استاد، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه تربیت مدرس | ||
| 4استاد، مؤسسه تحقیقات جنگلها و مراتع کشور | ||
| 5دانشیار، گروه ژنتیک، دانشکده علوم زیستی، دانشگاه تربیت مدرس | ||
| 6استادیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه تربیت مدرس | ||
| چکیده | ||
| تأثیر متیلجاسمونات بر فعالیت آنزیمهای فنیلآلانینآمونیولیاز (PAL) و 4-کوماراتکوآلیگاز (4CL)، مقدار فنل کل و مقدار پروتئین کل در گیاه دارویی آگاستاکه (Agastache foeniculum [Pursh] Kuntze) بررسی شد. آزمایشها در قالب طرح کاملاً تصادفی و در شرایط هیدروپونیک انجام شد و گیاهان در معرض غلظتهای مختلف متیلجاسمونات (0، 1/0 و 1 میلیمولار) قرار گرفتند. بعد از گذشت 24 ساعت از شروع تیمار، فعالیت آنزیم PAL در گیاهان تیمار شده با 1 میلیمولار متیل جاسمونات، در مقایسه با گیاهان تیمار شده با 1/0 میلیمولار متیلجاسمونات و گیاهان شاهد، بهطور معنیداری افزایش یافت. فعالیت آنزیم 4CL نیز بعد از گذشت 24 ساعت از تیمار با 1 میلیمولار متیلجاسمونات، در مقایسه با تیمار 1/0 میلیمولار متیلجاسمونات و تیمار شاهد، بهطور معنیداری افزایش یافت. با وجود این تیمارهای متیلجاسمونات هیچ اثر معنیداری بر مقدار فنل کل بعد از گذشت 8، 12 و 24 ساعت از تیمار در مقایسه با تیمارهای شاهد هر یک از زمانهای یاد شده نداشتند. مقدار پروتئین کل با تیمارهای 1 یا 1/0 میلیمولار متیلجاسمونات بعد از گذشت 24 ساعت از تیمار در مقایسه با شاهد، بهطور معنیداری افزایش یافت. | ||
| کلیدواژهها | ||
| متیلجاسمونات؛ آگاستاکه (Agastache foeniculum [Pursh] Kuntze)؛ فنیلآلانینآمونیالیاز؛ 4-کوماراتکوآلیگاز؛ مواد فنلی | ||
| عنوان مقاله [English] | ||
| Effect of methyl jasmonate on metabolic enzymes and phenolics, in Agastache foeniculum [Pursh] Kuntze | ||
| نویسندگان [English] | ||
| F. Raouf Fard1؛ M. Sharifi2؛ R. Omidbaigi3؛ F. Sefidkon4؛ M. Behmanesh5؛ N. Ahmadi6 | ||
| 1PhD. Student, Department of Horticulture, College of Agriculture, Tarbiat Modares University, Tehran, Iran | ||
| 2Department of Plant Biology, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran | ||
| 3Department of Horticulture, College of Agriculture, Tarbiat Modares University, Tehran, Iran | ||
| 4Research Institute of Forests and Rangelands, Tehran, Iran | ||
| 5Department of Genetics, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran | ||
| 6Department of Horticulture, College of Agriculture, Tarbiat Modares University, Tehran, Iran | ||
| چکیده [English] | ||
| The effect of methyl jasmonate (MeJa) upon Agastache foeniculum [Pursh] Kuntze was investigated in aspects of enzymatic activities of phenylalanine ammonialyase (PAL) and 4-coumarate:CoA ligase (4CL), total phenolic content and total protein. The experiments were carried out based on a completely randomized design under a hydroponic system. The plants were subjected to different concentrations of MeJa (0, 0.1 and 1 mM). The PAL activity significantly increased in plants treated with 1 mM MeJa for 24 h compared with 0.1mM MeJa and control. In addition, 4CL activity also significantly increased at 24 h after 1mM MeJa treatment compared with 0.1mM MeJa treatment and control. However, MeJa treatments did not have any significant effect on total phenolic compounds after 8, 12 or 24 h of treatment compared with controls of those times. Total protein content significantly increased with 1 or 0.1 mM MeJa treatments after 24h of treatment compared with control plants. | ||
| کلیدواژهها [English] | ||
| methyl jasmonate, Agastache foeniculum [Pursh] Kuntze, phenylalanine ammonialyase, 4-Coumarate CoA ligase, Phenolic compounds | ||
| مراجع | ||
|
- امیدبیگی، ر. و محمودی سورستانی، م.، 1389. اثر تنش خشکی بر برخی صفات مرفولوژی، میزان و عملکرد اسانس گیاه گل مکزیکی Agastache foeniculum [Pursh] Kuntze. علوم باغبانی ایران (علوم کشاوری ایران)، 41(2): 161-153. - رضایی، ا.، قناتی، ف. و بهمنش، م.، 1390. افزایش تولید و آزادسازی تاکسول توسط متیلجاسمونات، امواج فراصوت و دیبوتیل فتالات در کشت سلولی فندق (Corylus avellana L.). زیستشناسی گیاهی، 3(7): 71-55. - Bi, H.H., Zeng, R.S., Su, L.M., An, M. and Luo, S.M., 2007. Rice allelopathy induced by methyl jasmonate and methyl salicylate. Journal of Chemical Ecology, 33(5): 1089-1103. - Biondi, S., Scaramagli, S., Capitani, F., Altamura, M.M. and Torrigiani, P., 2001. Methyl jasmonate upregulates biosynthetic gene expression, oxidation and conjugation of polyamines, and inhibits shoot formation in tobacco thin layers. Journal of Experimental Botany, 52(355): 231-242. - Bradford, M.H., 1976. Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1-2): 248-254. - Charles, D.J., Simon, J.E. and Widrlechner, M.P., 1991. Characterization of essential oil of Agastache species. Journal of Agricultural and Food Chemistry, 39(11): 1946-1949. - Choi, D.W., Jung, J.D., Ha, Y.I., Park, H.W., In, D.S., Chung, H.J. and Liu, J.R., 2005. Analysis of transcripts in methyl jasmonate-treated ginseng hairy roots to identify genes involved in the biosynthesis of ginsenosides and other secondary metabolites. Plant Cell Reports, 23(8): 557-566. - Ellard-Ivey, M. and Douglas, C.J., 1996. Role of jasmonates in the elicitor-and wound-inducible expression of defense genes in parsley and transgenic tobacco. Plant Physiology, 112(1): 183-192. - Fuentes-Granados, R.G. and Widrlechner, M.P., 1995. Diversity among and within populations of Agastache foeniculum. Proceedings of the 14th Annual North American Prairie Conference: Prairie Biodiversity, Kansas State University, Manhattan, Kansas, 12-16 July, 1994: 1-8. - Gang, D.R., Wang, J.H., Dudareva, N., Nam, K.H., Simon, J.E., Lewinsohn, E. and Pichersky, E., 2001. An investigation of the storage and biosynthesis of phenylpropenes in sweet basil. Plant Physiology, 125(2): 539-555. - Garden, H., 2003. Biotechnological production of podophyllotoxin by Linum album suspension cultures. Doctoral Thesis, Heinrich-Heine Universität, Düsseldorf. - González-Aguilar, G.A., Tiznado-Hernández, M.E., Zavaleta-Gatica, R. and Martínez-Téllez, M.A., 2004. Methyl jasmonate treatments reduce chilling injury and activate the defense response of guava fruits. Biochemical and Biophysical Research Communications, 313(3): 694-701. - Gundlach, H., Mueller, M.J., Kutchan, T.M. and Zenk, M.H., 1992. Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. Proceeding of the National Academy of Science of the United States of America, 89(6): 2389-2393. - Heredia, J.B. and Cisneros-Zevallos, L., 2009. The effect of exogenous ethylene and methyl jasmonate on PAL activity, phenolic profiles and antioxidant capacity of carrots (Daucus carota) under different wounding intensities. Postharvest Biology and Technology, 51(2): 242-249. - Kim, H.J., Chen, F., Wang, X. and Choi, J.H., 2006. Effect of methyl jasmonate on phenolics, isothiocyanate, and metabolic enzymes in radish sprout (Raphanus sativus L.). Journal of Agricultural and Food Chemistry, 54(19): 7263-7269. - Kim, H.J., Fonseca, J.M., Choi, J.H. and Kubota, C., 2007. Effect of methyl jasmonate on phenolic compounds and carotenoids of romaine lettuce (Lactuca sativa L.). Journal of Agricultural and Food Chemistry, 55(25): 10366-10372. - Lee, D. and Douglas, C.J., 1996. Two divergent members of a tobacco 4-coumarate: coenzyme A ligase (4CL) gene family. cDNA structure, gene inheritance and expression, and properties of recombinant proteins. Plant Physiology, 112(1): 193-205. - Maciejewska, B.D., Kesy, J., Zielinska, M. and Kopcewicz, J., 2004. Jasmonates inhibit flowering in short-day plant pharbitis nil. Plant Growth Regulation, 43: 1-8. - Maizura, M., Aminah, A. and Wan Aida, W.M., 2011. Total phenolic content and antioxidant activity of kesum (Polygonum minus), ginger (Zingiber officinale) and turmeric (Curcuma longa) extract. International Food Research Journal, 18(2): 529-534. - Mallavarapu, G.R., Kulkarni, R.N., Baskaran, K. and Ramesh, S., 2004. The essential oil composition of anise hyssop grown in India. Flavour and Fragrance Journal, 19(4): 351-353. - Mazza, G. and Kiehn, F.A., 1992. Essential oils of Agastache foeniculum, a potential source of methyl chavicol. Journal of Essential Oil Research, 4(3): 295-299. - Memelink, J., 2009. Regulation of gene expression by jasmonate hormones. Phytochemistry, 70(13-14): 1560-1570. - Mizukami, H., Tabira, Y. and Ellis, B.E., 1993. Methyl jasmonate-induced rosmarinic acid biosynthesis in Lithospermum erythrorhizon cell suspension cultures. Plant Cell Reports, 12(12): - Omidbaigi, R., Kabudani M. and Khoorang M., 2008. Nitrogen fertilizer affecting herb dry yield, essential oil content and compositions of Agastache foeniculum Pursh. Journal of Essential Oil Bearing Plants, 11(3): 261-266. - Omidbaigi, R. and Mahmoodi, M., 2010. Effect of irrigation regimes on the essential oil content and composition of Agastache foeniculum. Journal of Essential Oil Bearing Plants, 13(1): 59-65. - Omidbaigi, R. and Sefidkon, F., 2003. Essential oil composition of Agastache foeniculum cultivated in Iran. Journal of Essential Oil Research, 15(1): 52-53. - Qian, Z.G., Zhao, Z.J., Xu, Y., Qian, X. and Zhong, J.J., 2004. Novel chemically synthesized hydroxyl-containing jasmonates as powerful inducing signals for plant secondary metabolism. Biotechnology and Bioengineering, 86(7): 809-816. - Sharan, M., Taguchi, G., Gonda, K., Jouke, T., Shimosaka, M., Hayashida, N. and Okazaki M., 1998. Effects of methyl jasmonate and elicitor on the activation of phenylalanine ammonia-lyase and the accumulation of scopoletin and scopolin in tobacco cell cultures. Plant Science, 132: 13-19. - Song, J. and Wang, Z., 2009. Molecular cloning, expression and characterization of a phenylalanine ammonia-lyase gene (SmPAL1) from Salvia miltiorrhiza. Molecular Biology Reports, 36(5): 939-952. - Szabo, E., Thelen, A. and Petersen, M., 1999. Fungal elicitor preparations and methyl jasmonate enhance rosmarinic acid accumulation in suspension cultures of Coleus blumei. Plant Cell Reports, 18(6): 485-489. - Tuan, P.A., Li, X., Park, N.I., Lee, S.Y., Kim, H.H. and Park, S.U., 2011. Molecular cloning of 4-coumarate: CoA ligase and total phenolic content in garlic (Allium sativum). Plant Omics, 4(1): 25-28. - Wakabayashi, K., Hoson, T. and Kamisaka, S., 1997. Osmotic stress suppresses cell wall stiffening and the increase in cell wall-bound ferulic and diferulic acids in wheat coleoptiles. Plant Physiology, 113(3): 967-973. - Weitzel, C. and Petersen, M., 2010. Enzymes of phenylpropanoid metabolism in the important medicinal plant Melissa officinalis L. Planta, 232(3): 731-742. - Wu, J. and Lin, L., 2003. Enhancement of taxol production and release in Taxus chinensis cell cultures by ultrasound, methyl jasmonate and in situ solvent extraction. Applied Microbiology and Biotechnology, 62(2-3): 151-155. - Xu, H., Park, N.I., Li, X., Kim, Y.K., Lee, S.Y. and Park, S.U., 2010. Molecular cloning and characterization of phenylalanine ammonia-lyase, cinnamate 4-hydroxylase and genes involved in flavone biosynthesis in Scutellaria baicalensis. Bioresource Technology, 101(24): 9715-9722. - Yamamura, Y., Ogihara, Y. and Mizukami, H., 2001. Cinnamic acid 4-hydroxylase from Lithospermum erythrorhizon: cDNA cloning and gene expression. Plant Cell Reports, 20(7): 655-662. - Yao, H. and Tian, S., 2005. Effects of pre-and post-harvest application of salicylic acid or methyl jasmonate on inducing disease resistance of sweet cherry fruit in storage. Postharvest Biology and Technology, 35(3): 253-262. | ||
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