Related Links
The effects of plant growth regulators and explants on callogenesis, regeneration and suspension culture in Foeniculum vulgare Mill. | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| Article 8, Volume 25, Issue 3 - Serial Number 45, November 2009, Pages 364-375 PDF (303.46 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/ijmapr.2009.7173 | ||
| Authors | ||
| P. Sarkheil1; M. Omidi* 2; S.A. Peyghambari2; S. Davazdahemami3 | ||
| 1Agriculture and Natural Resources of Tehran University, Iran | ||
| 2Department of Agriculture, Tehran University, Iran | ||
| 3Agricultural and Natural Resources Research Center of Esfahan, Iran | ||
| Abstract | ||
| Seeds were cultured on Whatman paper by sterile water in cube, solid MS and solid MS. Seeds were not germinated on medium but 80% of seeds were germinated on Whatman paper, so this method is used as a basic method. Seeds were germinated after four days and after two weeks of culture they had normal roots, shoots and leaves. Cultures were incubated at 25◦ ±2◦C and exposed to 16 hours light per day. Explants were cultured on MS medium supplemented with 3% sucrose and solidified with 0.8% (w/v) agar. The pH of the medium was adjusted to 5.8 before autoclaving. Different types of explants were used for this experiment; root, crown, apical meristem, hypocotyls and leaf. Between different kinds of explants leaf didn’t response to callogenesis. The effects of different combinations of 2,4-D (2,4-Diclorophenoxy acetic acid) and BAP (6-Benzylaminopurine) were studied. Subculture was done every 3 weeks. In order to determine regeneration ability, the initiated callus were transferred to a regeneration medium which was composed of macronutrient, micronutrient and organic components of MS, 2,4-D (0.2 mgL-1), BAP (0.5, 2, 4, 15, 20 and 25 mgL-1) and MS without hormones, 0/3% sucrose, pH 5.8 for 4 weeks. In the presence of 2,4-D (2 and 4 mgL-1) and BAP (0.25 and 0.5 mgL-1) in the callus induction medium, high callus production percentage was reported. The hypocotyls, in contrast to the primary leaf explants, and apical meristem segments were more responsive to the tested combinations of 2,4-D and BAP. The callus from all explants was soft, watery and loose friable. During subculture period, hypocotyls and apical meristem were proliferated more on medium with the addition of (0.25 and 0.5 mgL-1) BAP and (2 and 4 mgL-1) 2,4-D than the medium contain BAP (1 mgL-1) and 2,4-D (8 mgL-1). The present study, in F. vulgare MS media without any hormone was sufficient to regenerate the plantlet from the hypocotyls, roots and apical meristems explants. In MS medium supplemented with BAP (0.5, 2 and 4 mgL-1) and 2,4-D (0.2 mgL-1) shoots were formed earlier when the number of subculture was increased 4 times. | ||
| Keywords | ||
| Fennel; plant regeneration; Callogenesis; growth regulators | ||
| References | ||
|
- Anzidei, M., Vivona, L., Schiff, S. and Bennici, A., 1996. In vitro culture of Foeniculum vulgare: Callus characteristics in relation to morphogenesis. Plant Cell, Tissue and Organ Culture, 45: 263-268. - Anzidie, M., Bennici, A., Schiff, S., Tan, C. and Mori, B., 2000. Organogenesis and somatic embryogenesis in Foeniculum vulgare: histological observation of developing embryogenesis callus. Plant Cell, Tissue and Organ Culture, 61: 69-79. - Bennici, A., Anzidei, M. and Vendramin, G., 2004. Genetic stability and uniformity of Foeniculum vulgare Mill. Regeneration of plants through organogenesis and somatic embryogenesis. Plant Science, 166: 221-227. - Hanault, G. and Mattar, A., 1995. Enhancement of somatic embryogenesis frequency by gibberellic acid in fennel. Plant Cell, Tissue and Organ Culture, 41: 171-176. -Hanault, G. and Manoir, J., 1992. Micro propagation of fennel (Foeniculum vulgare Mill.). Biotechnology in Agriculture and Forestry, 19: 199-217. - Lawless, J., 1992. The Encyclopedia of Essential oils , Element Books Lt, Shaftesbur ,United Of Kigdom, 256 pages. - Stahl, E., Dumont, E. and Jork, H., 1975. Analyze chromatograhique et Microscopique des drogues. Technique et Documentation, pp: 148-149. - Theiler Hedtrich, R. and Kagi, A.C., 1991. Cloning in vitro & somatic embryogenesis in Foeniculum vulgare Mill. (fennel) of 'Zeta fino' and 'Zefa tard'. Acta Horticulture, 300: 287-291. | ||
|
Statistics Article View: 2,350 PDF Download: 1,060 |
||
Journal management system. designed by sinaweb