| تعداد نشریات | 282 |
| تعداد نشریات سازمان | 80 |
| تعداد شماره‌ها | 3,251 |
| تعداد مقالات | 35,917 |
| تعداد مشاهده مقاله | 54,570,323 |
| تعداد دریافت فایل اصل مقاله | 93,692,950 |
GENETIC VARIATION AMONG NATURAL POPULATIONS OF AGRPYRON CRISTAUM (POACEAE) BASED ON SDS-PAGE OF SEED PROTEINS | ||
| The Iranian Journal of Botany | ||
| مقاله 7، دوره 19، شماره 2، اسفند 2013، صفحه 186-193 اصل مقاله (759.83 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22092/ijb.2013.4156 | ||
| نویسندگان | ||
| Mehdi Yousofi* 1؛ Mehrnoosh Esmaeili1؛ Mahmood Otroshy2 | ||
| 1Department of Biology, Payam Noor University, Tehran, Iran. | ||
| 2Agricultural Biotechnology Research Institute -Central region of Iran (ABRII) | ||
| چکیده | ||
| SDS-PAGE of seed proteins in 11 natural populations of Agropyron cristatum and one cultivated population of Elymus junceus was studied. In total 31 protein bands of different molecular weights were detected on the polyacrylamide gel indicating a high diversity. By performing Cluster Analysis and Principle Coordinate Analysis on these data, all studied populations were grouped in three major clusters. Clear correlation was seen between the population in three clusters with their geographical localities. This result suggests that seed protein markers between the natural populations of Agropyron cristatum are mainly correlated with eco-geographical factors and cannot be used alone for its taxonomy in the infra-specific levels. | ||
| کلیدواژه‌ها | ||
| Agropyron cristatum؛ cluster analysis؛ Iran؛ SDS PAGE؛ Seed protein | ||
| عنوان مقاله [English] | ||
| تنوع ژنتیکی بین جمعیت های خودروی Agropyron cristatum براساس الکتروفورز پروتئین‌های دانه | ||
| نویسندگان [English] | ||
| مهدی یوسفی1؛ مهرنوش اسماعیلی1؛ محمود اوتروشی2 | ||
| 1دانشیار گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه پیام نور، تهران. | ||
| 2مؤسسه تحقیقات فناوری کشاورزی، منطقه مرکزی ایران، نجف آباد. | ||
| چکیده [English] | ||
| پروتئین‌های ذخیره‌ای دانه 11 جمعیت خودروی Agropyron cristatum و یک جمعیت کاشته شدهElymus junceus از طریق الکتروفورز روی ژل پلی آکریل آمید بررسی شدند. در مجموع 31 باند پروتئینی با وزنهای مولکولی متفاوت روی ژل ظاهر شد که نشانگر تنوع بالا است. با انجام آنالیز خوشه‌ای و آنالیز مولفه‌های اصلی روی داده‌های به دست آمده، تمام جمعیت‌های مورد مطالعه در سه خوشه گروه بندی شدند. ارتباط آشکاری بین تاکسونهای هر خوشه و مکانهای جغرافیائی آنها مشاهده شد. نتایج پیشنهاد می‌کند که مارکرهای پروتئینی دانه جمعیت های خودروی Agropyron cristatum عمدتا با عوامل اکوجغرافیائی همبستگی دارند و به تنهائی نمی‌توانند برای تاکسونومی این گونه در سطوح زیرگونه‌ای مفید واقع شوند. | ||
| کلیدواژه‌ها [English] | ||
| Agropyron cristatum, cluster analysis, Iran, SDS PAGE, Seed protein | ||
| مراجع | ||
|
Aiken, S. G., Gardiner, S. E., Bassett, H. C. M., Wilson, B. L. & Consaul, L. L. 1998: Implications from SDS-PAGE analyses of seed proteins in the classification of taxa of Festuca and ¸Lolium (Poaceae). -Biochemical Systematics and Ecology 26: 511-533. Assadi, M. 1995: Meiotic configuration and chromosome number in some Iranian species of Elymus. -Bot J. Linn. Soc. 117: 159-168. Bor, N. L. 1970: Gramineae, in. Rechinger K. H. (ed.), Flora Iranica, no. 70. -Akademische Deyek.ll, Verlagsoa Nstalt Graz, -Austria. Che, Y. H. & Li, L. H. 2007: Genetic diversity of prolamines in Agropyron mongolicum Keng indigenous to northern China. -Genetic Resources and Crop Evolution 54:1145–1151. Chen, L., Ficher H. & Jensen, U. 1997: Accumulation of seed storage proteins and the taxonomy of Poaceae. -Pl. Syst. Evol. 206: 243-257. Crawford, D. J. 1990: PlantMolecularSystematics. -New York. Dewey, D. R. & Assay, K. H. 1975: The crested wheatgrasses of Iran. -Crop Science 15: 844-849. Dewey, D. R. 1984: The genome system of classification as a guid to intergeneric hybridization with the perennial Triticeae. In. Gustafson JP. ed. Gene manipulation in plant improvement. -Plenum Publishing Corporation, New York. Dewey, D. R. 1986: Taxonomy of the crested wheatgrass (Agropyron). In. Jonson, K. L. (ed.) Crested wheatgrass: its values, problems and myths. -Utah State University, Logan. Gardiner, S. E. & Forde, M. B. 1992: Identification of cultivars of grasses, forage and legumes by SDS-PAGE of seed proteins. In. Seed Analysis (eds. Linskens, H. F. & Jackson, J. F.), Springer-Verlag, -Germany. Hames, B. D. & Rickwood, D., 1990: One-dimensional polyacrylamide gel electrophoresis. In. Gel electrophoresis of proteins, 2nd Edition, Oxford University Press, -New York. Jensen, U.& Grumpe, B. 1983: Seed storage proteins. In. Jensen, U. & Fairbrothers, D.E. (eds) Proteins and nucleic acids in plant systematics. -Springer-Verlag Inc., Berlin. Love, A. 1984: Conspectus of the Triticeae. -Feddes Repertium 95 (7-8): 425-521. Melderis, A. 1985: Agropyron Gaertner in. Davis, P.H. (ed.), Flora of Turkey and the East Aegean Islands, Vol. 9: 204-206. -Edinburgh University Press, Edinburgh. Moustakas, M., Symeonidis, L. & Coucoli, H. 1986: Seed protein electrophoresis in Agropyron junceum (L.) P. B. Complex. -Annals of Botany 57 (1): 35-40. Nevo, E., Apelbaum-Elkaher, I., Garty, J. & Beiles, A. 1997: Natural selection causes microscale allozyme diversity in wild barley and a lichen at 'Evolution Canyon', Mt. Carmel, Israel. -Heredity 78: 373–382. Rafezi, A., Farshadfar, M. & Farshadfar, A. 2008: Using biochemical markers (proteins) for study interspecies diversity at 17 populations of Agropyron elongatum L. -Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research 16: 248- 253. Rasband, W. 1997: ImageJ, 1.41o, -National Institute of Health, USA. http://rsb.info.nih.gov/ Saraswati, R., Matoh, T., Sasai, T., Phupaibul, P., Lumpkin, T., Kobayashi, M. & Sekiya, J. 1993: Identification of Sesbania species from electrophoretic patterns of seed proteins. -Trop. Agric. 70: 282-285. Sheidai, M., Saeidi, S. & Atri, M. 2008: Taxonomic applications of seed proteins in the genus Bromus L. (Poaceae).-Iran. J. Bot. 14 (2): 126-131. Skrajna, T., kubicka, H. & Rzymowska, Z. 2012: Phenotypic variation in relation to seed storage protein polymorphism in Bromus secalinus L. (Gramineae) populations from North-Eastern Poland. -Polish Journal of Ecology 91): 41–55. Sneath, P. H. A. & Sokal, R. R. 1973: Numerical taxonomy; the principles and practice of numerical classification. -W. H. Freeman Publisher, San Francisco. StatSoft, Inc. 2007: STATISTICA (data analysis software system), version 8.0. -www.statsoft.com. Sun, G.L., Díaz, O., Salomon, B., & von Bothmer, R. 1999: Genetic diversity in Elymus caninus as revealed by isozyme, RAPD, and microsatellite markers. -Genome 42 (3): 420-431. Taghizadeh, R., Jafari, A. A., Choukan, R. & Asghari, A. 2009: Investigation of genetic diversity in crested wheatgrass (Agropyron cristatum (L.) Garetn. populations using RAPD molecular markers. -Journal on Plant Science Researches 14 (2): 8-18. Tamkoc, A. & Arslan, E. 2011: Inter and intra-specific variation in SDS-PAGE of seed proteins of three Poa L. (Poaceae) species. -Pakistan Journal of Botany 43 (2): 1105-1110. Tzvelev, N. N. 1976: Poaceae URSS. Tribe 3. Triticeae Dum. Genus 17. Agropyron. -Leningrad: USSR Academy of Science Press. Wei-dong, J., Na, L. & De-lin, H. 2006: Genetic diversity of seed storage proteins in different ecotype varieties of Japonica rice and its application. -Rice Science 13 (2): 85-92. Yousofi, M. & Aryavand, A. 2004: Determination of ploidy levels of some poulations of Agropyron cristatum (Poaceae) in Iran by flow cytometry. -Iranian Journal of Science & Technology, Transaction A 28 (A1): 137-143. Yousofi, M. & Assadi, M. 2006: Tow new taxa of Agropyron cristatum (Poaceae) for the flora of Iran. –Iran. Journ. Bot. 12 (1): 87-89. | ||
|
آمار تعداد مشاهده مقاله: 2,142 تعداد دریافت فایل اصل مقاله: 1,861 |
||