Identification of molecular markers linked to sugarbeet cyst nematode resistance gene(s) | ||
| چغندرقند | ||
| Article 4, Volume 28, Issue 2, February 2013, Pages 158-149 PDF (324.63 K) | ||
| Document Type: Scientific - Research | ||
| DOI: 10.22092/jsb.2013.1726 | ||
| Authors | ||
| N. Rahmani* 1; M. Mesbah2; P. Norouzi3 | ||
| 1M.Sc of Islamic Azad University, Science and Research Branch. Tehran, Iran. | ||
| 2Professor of Seed and Plant Certification and Registation Institute, Iran. | ||
| 3Assistant professor of Sugar Beet Seed Institute (SBSI), Iran. | ||
| Abstract | ||
| Beet cyst nematode (Heterodera schachtii Schmidt). Spreads extensively in different regions of sugar beet cultivation, and therefore many efforts are accomplished to prevent decrease of sugar and root yield caused by the disease. The most appropriate control method of this disease is improvement of resistant varieties. Wild species of procumbentes in Beta are very important because they contain resistance genes against different diseases including beet cyst nematode. Ten-mer primers and specific sequences were selected to identify DNA molecular markers linked to beet cyst nematode resistance genes. For this purpose, at first, segregating populations for resistance genes were selected and after seed sowing in pots in greenhouse, seedlings were inoculated with 1000 nematode larva several times. Resistant plants (with lower than 10 cysts) and susceptible plants (with less than 10 cysts) were identified and divided to two groups. In the next step, RAPD-PCR, finger printing of the samples and comparison the DNA banding pattern of the two groups was done by using selected primers such as: OP-X-02, OP-X-15, OP-G-02, OP-D-13, OP-B-11, OP-Y-10 and Sat-121 specific primers and the oligonncleotides TGAACACCTTTCAAAT (forward) and CGTAAGAGACTATGA (Reverse) and one hundred primers from the operon kits. After calculating of correlation between resistance plants and the presence of special DNA band, OP-D-13 and Sat-121 molecular markers were identified to be linked to resistant genes against beet cyst nematode. In conclusion, these two molecular markers can be used for screening of resistant plants in laboratory conditions. | ||
| Keywords | ||
| Cyst nematode; resistance; molecular markers; sugar beet | ||
|
Supplementary Files
|
||
| References | ||
|
Ahmadi E, Hejarood Gh, Shrifi tehrani E, Kheiri A, Akhyani A. The first report of isolation and identification of Paecilomyces farinosus from cyst nematode of Heterodera schachtii and study it`s antagonistic effect on nematode eggs in Iran. Proceedings of the 12th Iranian Plant Protection Congress.1394. P354
Cai D, Kleine M, Kifle S, Harloff HJ, Sandal NN, Marcker KA, Lankhorst RMK, Salentijn EMJ, Lange W, Stiekema WJ, Wyss U, Grundler MW, Jung C. Positional cloning of a gene for nematode resistance in sugar beet. Science. 1997. 275: 832-834.
Hallden C, Sall T, Olsson K, Nilsson NO, Hjerdin A. The use of bulked segregant analysis to accumulate RAPD markers near a locus for beet cyst nematode resistance in Beta vulgaris. Plant Breed. 1997. 116: 18-22.
Jassem B. Apomixis in the genus Beta. Apomixis News Letter. 1990. 2:7-23.
Kleine M, Voss H, Cai D, Jung C. Evaluation of nematode resistant sugar beet (Beta vulgaris L.) lines by molecular analysis. T. A. G. 1998. 97: 896-904.
Lange W, De Bock TSM. Pre-breeding for nematode resistance in beet, J. of Sugar Beet Res. 1994. 31:13-26.
Mesbah M. Characterisation of Alien Chromosomes in Monosomic Additions of Beta. Ph.D. thesis. Wageningen .Agricultural University. 1997.
Mesbah M, Bock TSM, Sandbrink JM, Lankhorst RMK, Lange W. Molecular and morphological characterization of monosomic additions in Beta vulgaris, carrying extra chromosomes of B. procumbens or B. patellaris. Mol. Breed. 1997. 3: 147-157.
Mohamadigoltapei A, Pakdamansardroodi B, Rezaeedanesh y. Diseases caused by biotic and Sugar beet disturbances. Tarbiat Modarres University Press. 1998. Pp 84-89
Norouzi P. The Simple and effective method for Genomic DNA extraction from plant and Fungus for PCR-based analyses. 2003. Pp 175-176
Salentijn EMJ, Sandal NN, Lankhorst RK, Lange W, Bock SMD, Marcker KA, Stiekema WJ. Long range organization of a satellite DNA family flanking the beet cyst nematode resistance locos(Hs1) on chromosome-1 of B. patellaris and B. procumbens. Theoretical and Applied Genetics. 1994. 89: 459-466.
Salentijn EMJ. Molecular characterization of the beet cyst nematode( Heterodera schachtii) resistance locus Hs1. Ph.D Thesis. Wageningen University. Netherlands. 1995. Pp:75-88.
Sandal NN, Salentijn EMJ, Kleine M, Cai D, Reuver M R, Druten MV, Bock TSM, Lange W, Steen P, Jung C, Marcker K, StiekemaWJ and Lankhorst RMK. Backcrossing of nematode-Resistant sugarbeet: a second nematode resistance gene at the Locus containing Hs1pro-1. Mol. Breedg. 1997. 3: 471-480.
Whitney ED, Duffus E. Compendium of Beet Diseases and Insects, APS press. 1991. 76p. | ||
|
Statistics Article View: 4,060 PDF Download: 3,372 |
||
Journal management system. designed by sinaweb