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Yazdanizad, Maryam, Saboor Yaraghi, Ali Akbar, Javdani Shahedin, Golshid, Asadian, Mahla, Mazaheri Nezhad Fard, Ramin. (1404). Comparative Analysis of Two Consecutive Genome Sequencing Results of Enterococcus Faecium Strain Entfacye. سامانه مدیریت نشریات علمی, 80(5), 1321-1330. doi: 10.32598/ARI.80.5.3339
Maryam Yazdanizad; Ali Akbar Saboor Yaraghi; Golshid Javdani Shahedin; Mahla Asadian; Ramin Mazaheri Nezhad Fard. "Comparative Analysis of Two Consecutive Genome Sequencing Results of Enterococcus Faecium Strain Entfacye". سامانه مدیریت نشریات علمی, 80, 5, 1404, 1321-1330. doi: 10.32598/ARI.80.5.3339
Yazdanizad, Maryam, Saboor Yaraghi, Ali Akbar, Javdani Shahedin, Golshid, Asadian, Mahla, Mazaheri Nezhad Fard, Ramin. (1404). 'Comparative Analysis of Two Consecutive Genome Sequencing Results of Enterococcus Faecium Strain Entfacye', سامانه مدیریت نشریات علمی, 80(5), pp. 1321-1330. doi: 10.32598/ARI.80.5.3339
Yazdanizad, Maryam, Saboor Yaraghi, Ali Akbar, Javdani Shahedin, Golshid, Asadian, Mahla, Mazaheri Nezhad Fard, Ramin. Comparative Analysis of Two Consecutive Genome Sequencing Results of Enterococcus Faecium Strain Entfacye. سامانه مدیریت نشریات علمی, 1404; 80(5): 1321-1330. doi: 10.32598/ARI.80.5.3339

Comparative Analysis of Two Consecutive Genome Sequencing Results of Enterococcus Faecium Strain Entfacye

Archives of Razi Institute
مقاله 25، دوره 80، شماره 5، آذر و دی 2025، صفحه 1321-1330    اصل مقاله (775.53 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32598/ARI.80.5.3339
نویسندگان
Maryam Yazdanizad1؛ Ali Akbar Saboor Yaraghi2؛ Golshid Javdani Shahedin3؛ Mahla Asadian4؛ Ramin Mazaheri Nezhad Fard* 2، 4
1Department of Medical Biotechnology, TMS.C., Islamic Azad University, Tehran, Iran.
2Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
3Department of Research and Development, Pasteur Institute, Tehran, Iran.
4Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
چکیده
Introduction: The overuse of antimicrobials in healthcare has driven the emergence, persistence, and rapid spread of antimicrobial-resistant pathogens. Vancomycin-resistant enterococci (VRE), mainly Enterococcus faecium, have recently emerged as multi drug-resistant (MDR) bacteria worldwide. Consequently, enterococcal infections have become more challenging to treat due to their increased multiple-drug resistance. Studying the genome of an enterococcal isolate and investigating genome changes over time help researchers better understand the development of antimicrobial resistance in bacterial isolates.
Materials & Methods: In the present study, E. faecium EntfacYE isolate from a human biological sample was assessed. After phenotypic, biochemical,and molecular verifications of the bacterial isolate, the bacterial genome was completely sequenced.
Results: In total, the EntfacYE genomic subsystems contained 23 various categories with 46 antimicrobial resistance genes. In the current study, 31 antimicrobial resistance genes were reported in the subsystems, and 15 genes had no subsystems. Genes conferring tetracycline resistance were reported in this study. Increases in the number and type of antimicrobial resistance genes were recorded, indicating that bacteria are becoming rapidly resistant to available antimicrobials. 
Conclusion: In general, the study of antimicrobial resistance genes in bacteria can be effective in better understanding resistance patterns and mechanisms, which can lead to find novel protocols for limiting spread of antimicrobial resistance in bacteria.
کلیدواژه‌ها
Whole-genome sequencing؛ Enterococcus faecium؛ Biological samples؛ Antimicrobial resistance
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References

  1. Wozniak-Biel A, Bugla-Płoskońska G, Burdzy J, Korzekwa K, Ploch S, Wieliczko A. Antimicrobial resistance and iofilm formation in Enterococcus spp. isolated from humans and turkeys in Poland. Microb Drug Resist. 2019; 25(2):277-86. [DOI:10.1089/mdr.2018.0221] [PMID]
  2. Khare T, Anand U, Dey A, Assaraf YG, Chen ZS, Liu Z, et al. Exploring phytochemicals for combating antibiotic resistance in microbial pathogens. Front Pharmacol. 2021; 12:720726. [DOI:10.3389/fphar.2021.720726] [PMID]
  3. Forde BM, Bergh H, Cuddihy T, Hajkowicz K, Hurst T, Playford EG, et al. Clinical implementation of routine whole-genome sequencing for hospital infection control of multi-drug resistant pathogens. Clin Infect Dis. 2023; 76(3):e1277-84. [DOI:10.1093/cid/ciac726] [PMID]
  4. Marston HD, Dixon DM, Knisely JM, Palmore TN, Fauci AS. Antimicrobial resistance. JAMA. 2016; 316(11):1193-204. [DOI:10.1001/jama.2016.11764] [PMID]
  5. Lebreton F, Valentino MD, Schaufler K, Earl AM, Cattoir V, Gilmore MS. Transferable vancomycin resistance in clade B commensal-type Enterococcus faecium. J Antimicrob Chemother. 2018; 73(6):1479-86. [DOI:10.1093/jac/dky039] [PMID]
  6. Elahi Y, Nowroozi J, Fard RMN. Isolation and characterization of bacteriophages from wastewater sources on Enterococcus spp. isolated from clinical samples. Iran J Microbiol. 2021; 13(5):671-7. [DOI:10.18502/ijm.v13i5.7434] [PMID]
  7. Adeniji OO, Sibanda T, Okoh AI. Recreational water quality status of the Kidd’s Beach as determined by its physicochemical and bacteriological quality parameters. Heliyon. 2019; 5(6):e01893. [DOI:10.1016/j.heliyon.2019.e01893] [PMID]
  8. Rapid Annotation using Subsystem Technology (RAST). online sequence analysis service [Internet]. 2025 [Updared 25 November 2025]. Available from: [Link]
  9. DNA Data Bank of Japan. sharing and analysis services for data from life science researches and advances science. Shizuoka: DDBJ Center; 2025. [Link]
  10. Pulingam T, Parumasivam T, Gazzali AM, Sulaiman AM, Chee JY, Lakshmanan M, et al. Antimicrobial resistance: Prevalence, economic burden, mechanisms of resistance and strategies to overcome. Eur J Pharm Sci. 2022; 170:106103. [DOI:10.1016/j.ejps.2021.106103] [PMID]
  11. Ayobami O, Willrich N, Reuss A, Eckmanns T, Markwart R. The ongoing challenge of vancomycin-resistant Enterococcus faecium and Enterococcus faecalis in Europe: An epidemiological analysis of bloodstream infections. Emerg Microbes Infect. 2020; 9(1):1180-93. [DOI:10.1080/22221751.2020.1769500] [PMID]
  12. Gorrie C, Higgs C, Carter G, Stinear TP, Howden B. Genomics of vancomycin-resistant Enterococcus faecium. Microb Genom. 2019; 5(7):e000283. [DOI:10.1099/mgen.0.000283] [PMID]
  13. Sun L, Xu J, Wang W, He F. Emergence of vanA-type vancomycin-resistant Enterococcus faecium ST 78 strain with a rep2-type plasmid carrying a Tn1546-like element isolated from a urinary tract infection in China. Infect Drug Resist. 2020; 13:949-55. [DOI:10.2147/IDR.S247569]
  14. Berglund F, Böhm ME, Martinsson A, Ebmeyer S, Österlund T, Johnning A, et al. Comprehensive screening of genomic and metagenomic data reveals a large diversity of tetracycline resistance genes. Microb Genom. 2020; 6(11):mgen000455. [DOI:10.1099/mgen.0.000455] [PMID]
  15. Tarrah A, Pakroo S, Corich V, Giacomini A. Identification and transferability of tetracycline resistance in Streptococcus thermophilus during milk fermentation, storage, and gastrointestinal transit. Fermentation. 2021; 7(2):65. [DOI:10.3390/fermentation7020065]
  16. Revtovich AV, Tjahjono E, Singh KV, Hanson BM, Murray BE, Kirienko NV. Development and characterization of high-throughput Caenorhabditis elegans-Enterococcus faecium infection model. Front Cell Infect Microbiol. 2021; 11:667327. [DOI:10.3389/fcimb.2021.667327] [PMID]
  17. Markwart R, Willrich N, Haller S, Noll I, Koppe U, Werner G, et al. The rise in vancomycin-resistant Enterococcus faecium in Germany: Data from the German Antimicrobial Resistance Surveillance (ARS). Antimicrob Resist Infect Control. 2019; 8:147. [DOI:10.1186/s13756-019-0594-3] [PMID]
  18. Sanderson H, Ortega-Polo R, Zaheer R, Goji N, Amoako KK, Brown RS, et al. Comparative genomics of multidrug-resistant Enterococcus spp. isolated from wastewater treatment plants. BMC Microbiol. 2020; 20(1):20. [DOI:10.1186/s12866-019-1683-4] [PMID]
  19. Amachawadi RG, Giok F, Shi X, Soto J, Narayanan SK, Tokach MD, et al. Antimicrobial resistance of Enterococcus faecium strains isolated from commercial probiotic products used in cattle and swine. J Anim Sci. 2018; 96(3):912-20. [DOI:10.1093/jas/sky056] [PMID]
  20. Bozdogan B, Berrezouga L, Kuo MS, Yurek DA, Farley KA, Stockman BJ, et al. A new resistance gene, linB, conferring resistance to lincosamides by nucleotidylation in Enterococcus faecium HM1025. Antimicrob Agents Chemother. 1999; 43(4):925-9. [DOI:10.1128/AAC.43.4.925] [PMID]
  21. Edirmanasinghe R, Finley R, Parmley EJ, Avery BP, Carson C, Bekal S, et al. A whole-genome sequencing approach to study cefoxitin-resistant Salmonella enterica serovar Heidelberg isolates from various sources. Antimicrob Agents Chemother. 2017; 61(4):e01919-16. [DOI:10.1128/AAC.01919-16] [PMID]
  22. Falagas ME, Vouloumanou EK, Samonis G, Vardakas KZ. Fosfomycin. Clin Microbiol Rev. 2016; 29(2):321-47. [DOI:10.1128/CMR.00068-15] [PMID]
  23. da Silva VL, Caçador NC, da Silva Cdos S, Fontes CO, Garcia GD, Nicoli JR, et al. Occurrence of multidrug-resistant and toxic-metal tolerant enterococci in fresh feces from urban pigeons in Brazil. Microbes Environ. 2012; 27(2):179-85. [DOI:10.1264/jsme2.ME11296] [PMID]
  24. Amachawadi R, Shelton N, Shi X, Vinasco J, Dritz S, Tokach M, et al. Selection of fecal enterococci exhibiting tcrb-mediated copper resistance in pigs fed diets supplemented with copper. Appl Environ Microbiol. 2011; 77(11). [DOI:10.1128/AEM.00364-11]
  25. Festa RA, Thiele DJ. Copper at the front line of the host-pathogen battle. PLoS Pathog. 2012; 8(9):e1002887.[DOI:10.1371/journal.ppat.1002887] [PMID]
  26. Zaidi SE, Zaheer R, Poulin-Laprade D, Scott A, Rehman MA, Diarra M, et al. Comparative genomic analysis of enterococci across sectors of the one health continuum. Microorganisms. 2023; 11(3):727. [DOI:10.3390/microorganisms11030727] [PMID]
  27. Pal C, Bengtsson-Palme J, Kristiansson E, Larsson DG. Co-occurrence of resistance genes to antibiotics, biocides and metals reveals novel insights into their co-selection potential. BMC Genomics. 2015; 16:964. [DOI:10.1186/s12864-015-2153-5] [PMID]
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