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بهینهسازی فرایند الکتروریسی نانوحامل ترکیبی برپایه پروتئین ماش (Vigna radiate L.)/ پلی وینیل الکل | ||
| تحقیقات مهندسی صنایع غذایی | ||
| دوره 23، شماره 1 - شماره پیاپی 76، شهریور 1403، صفحه 165-182 اصل مقاله (1.39 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/fooder.2025.365755.1392 | ||
| نویسندگان | ||
| رسول اسدزاده1؛ غلامرضا عسگری2؛ زهرا امام جمعه* 3؛ محمد اکرامی4 | ||
| 1گروه علوم و صنایع غذایی، پردیس بینالمللی ارس، دانشگاه تهران، تهران، ایران | ||
| 2عضو هیات علمی دانشکده کشاورزی دانشگاه تهران | ||
| 3پردیس کشاورزی و منابع طبیعی دانشگاه تهران | ||
| 4گروه علوم و صنایع غذایی - کرج - دانشگاه تهران | ||
| چکیده | ||
| در این تحقیق، از دستگاه الکتروریسی برای تولید نانوحامل ترکیبی بر پایۀ پروتئین ماش (VPr) و کمک پلیمر پلی وینیل الکل (PVA) استفاده شد. برای مدلسازی و بهینهسازی فرایند از روش سطح پاسخ (RSM) بر طبق متغیرهای ورودی شامل نسبت کمک پلیمر PVA (65 – 35 درصد وزنی)، سرعت پمپ (1.5 – 0.5 میلیلیتر بر ساعت)، فاصلۀ بین نوک سوزن تا جمع کننده (20 – 10 سانتیمتر) و ولتاژ اعمال شده (25 – 12.5 کیلوولت) استفاده گردید. طرح مرکب مرکزی (CCD) برای اجرا و تجزیه و تحلیل مدل استفاده شد و آنالیز ریختشناسی توسط میکروسکوپ الکترونی روبشی (SEM) نتایج بهینهسازی الکتروریسی را تأیید کرد. نتایج خواص محلول نانوحامل VPr-PVA از جمله ویسکوزیته (20.99 سانتی پوآز)، هدایت الکتریکی (254.18 میکروزیمنس بر سانتیمتر) و کشش سطحی (38.14 میلی نیوتن بر متر) تعیین گردید. بر این اساس، شرایط بهینه برای ساخت نانوحامل عملکردی VPr-PVA با قطر پیشبینی شده - صحت سنجی شده 332.9 - 345.7 نانومتر شامل غلظت 52.5 درصد وزنی نسبت کمک پلیمرPVA یک میلیلیتر بر ساعت سرعت پمپ، فاصلۀ 13.5 سانتیمتری بین نوک سوزن تا جمع کننده و 20 کیلوولت ولتاژ اعمالی ارزیابی شد. تجزیه و تحلیل SEM تأیید کرد که نانوحاملهای VPr-PVA در نقطۀ بهینه بدون مهره، دارای سطوح همگن، صاف و با توزیع نرمال هستند. بر طبق نتایج این تحقیق، پروتئین ماش پتانسیل تولید نانوفیبرهای یکنواخت و متخلخل و استفاده به عنوان نانو حامل ترکیبات زیست فعال را از خود نشان داد. | ||
| کلیدواژهها | ||
| الکتروریسی؛ Vigna radiate L؛ انکپسولاسیون؛ روش سطح پاسخ؛ بهینهسازی | ||
| عنوان مقاله [English] | ||
| Optimization of the electrospinning process of hybrid nanocarrier based on mung bean (Vigna radiate L.) protein / poly(vinyl alcohol) | ||
| نویسندگان [English] | ||
| Rasoul Asadzadeh1؛ Gholamreza Askari2؛ zahra Emam-djomeh3؛ Mohammad Ekrami4 | ||
| 1Department of Food Science and Industry, Ares International Campus, University of Tehran, Tehran, Iran | ||
| 2University of Tehran | ||
| 3Transfer Phenomena Laboratory (TPL). Institution: University of Tehran. Department: Department of Food Science and Engineering. | ||
| 4Department of Food Science and Technology- Karaj - University of Tehran | ||
| چکیده [English] | ||
| In this research, an electrospinning apparatus was used to fabricate a composite nanocarriers based on Vigna radiata L. protein (VPr) and polyvinyl alcohol (PVA) polymer. For modeling and optimization using the response surface method (RSM) with input variables including PVA polymer ratio (35-65%w/w), pump rate (0.5-1.5 mL/h), distance between needle tip and collector (10-20 cm) and applied voltage (12.5-25 kV) were used. Central composite design (CCD) was used to implement and analyze the model, and morphological analysis by scanning electron microscope (SEM) confirmed the electrospinning optimization results. The results of the properties of the VPr-PVA nanocarrier’s solution including viscosity (20.99 cp), electrical conductivity (254.18 µS/cm) and surface tension (38.14 mN/m) were determined. The optimal conditions for the production of VPr-PVA functional nanocarriers with the predicted diameter - verified 332.9 - 345.7 nm, including the concentration of 52.5%w/w PVA ratio, 1 mL/h, pump rate, 13.5 cm distance between the tip of the needle and the collector, 20 kV applied voltage was evaluated. SEM analysis confirmed that the VPr-PVA nanocarriers at the optimum point without beads have homogeneous, smooth and normally distributed surfaces. According to the results of this research, Vigna radiata L. protein showed the potential to produce uniform and porous nanofibers and use as a nanocarriers of bioactive compounds. | ||
| کلیدواژهها [English] | ||
| Electrospinning, Vigna Radiate L, Encapsulation, RSM, Optimization | ||
| مراجع | ||
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