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زینالی, کمیل, شریعتی, شایان, پوربابایی, احمد علی, شرفا, مهدی. (1403). ارزیابی کارایی باکتری‌های تجزیه‌کننده نفت و دارای توان حل‌کنندگی فسفات در فراهمی فسفر یک خاک آهکی آلوده نفتی. سامانه مدیریت نشریات علمی, 12(2), 191-212. doi: 10.22092/sbj.2024.366874.268
کمیل زینالی; شایان شریعتی; احمد علی پوربابایی; مهدی شرفا. "ارزیابی کارایی باکتری‌های تجزیه‌کننده نفت و دارای توان حل‌کنندگی فسفات در فراهمی فسفر یک خاک آهکی آلوده نفتی". سامانه مدیریت نشریات علمی, 12, 2, 1403, 191-212. doi: 10.22092/sbj.2024.366874.268
زینالی, کمیل, شریعتی, شایان, پوربابایی, احمد علی, شرفا, مهدی. (1403). 'ارزیابی کارایی باکتری‌های تجزیه‌کننده نفت و دارای توان حل‌کنندگی فسفات در فراهمی فسفر یک خاک آهکی آلوده نفتی', سامانه مدیریت نشریات علمی, 12(2), pp. 191-212. doi: 10.22092/sbj.2024.366874.268
زینالی, کمیل, شریعتی, شایان, پوربابایی, احمد علی, شرفا, مهدی. ارزیابی کارایی باکتری‌های تجزیه‌کننده نفت و دارای توان حل‌کنندگی فسفات در فراهمی فسفر یک خاک آهکی آلوده نفتی. سامانه مدیریت نشریات علمی, 1403; 12(2): 191-212. doi: 10.22092/sbj.2024.366874.268

ارزیابی کارایی باکتری‌های تجزیه‌کننده نفت و دارای توان حل‌کنندگی فسفات در فراهمی فسفر یک خاک آهکی آلوده نفتی

زیست شناسی خاک
مقاله 3، دوره 12، شماره 2، اسفند 1403، صفحه 191-212    اصل مقاله (1.32 M)
نوع مقاله: مقاله پژوهشی
شناسه دیجیتال (DOI): 10.22092/sbj.2024.366874.268
نویسندگان
کمیل زینالی1؛ شایان شریعتی* 2؛ احمد علی پوربابایی* 3؛ مهدی شرفا3
1گروه علوم و مهندسی خاک، دانشکدگان کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران
2گروه مهندسی محیط زیست، دانشکده محیط زیست، دانشگاه تهران، تهران، ایران.
3گروه مهندسی علوم خاک، دانشکدگان کشاورزی و منابع طبیعی دانشگاه تهران، کرج ، ایران
چکیده
در این پژوهش، با هدف بهبود زیست‌پالایی خاک‌ آلوده و افزایش فسفر دردسترس خاک، سویه‌های باکتریایی به روش غنی‌سازی از گل حفاری چاه نفتی با آلودگی بالای هیدروکربن‌های نفتی کل(Total Petroleum Hydrocarbon) (TPH) جدا شدند و توانایی آن‌ها در استفاده از نفت خام به عنوان تنها منبع کربن و انرژی مورد ارزیابی قرار گرفت. سپس توانایی انحلال فسفر و تولید سورفکتانت زیستی مورد بررسی قرار گرفت. در نهایت آزمایشی در محیط خاک به منظور بررسی انحلال فسفر طراحی گردید. این آزمایش در قالب طرح کاملا تصادفی با دو تیمارB (خاک آلوده به همراه تلقیح اجتماع سه جدایه باکتریایی) و C (شاهد، خاک آلوده بدون تلقیح باکتری) و شش تکرار انجام شد. تجزیه واریانس داده‌ها و رسم نمودارها به‌ترتیب با استفاده از نرم افزارهای Minitab و Excel انجام شد. نتایج نشان داد، از میان هفت باکتری جداشده از گل حفاری، جدایه‌های NW1،NW5 و NW7 به‌ترتیب با 6/20، 66/19 و 53/15 درصد تجزیه نفت در محیط MSM، بهترین عملکرد را در تخریب هیدروکربن‌ها داشتند. همچنین جدایه‌های NW7 و NW5 به‌ترتیب با شاخص انحلال 76/3 و 83/2 بیشترین میزان انحلال فسفات را نسبت به سایر جدایه‌ها ثبت کردند (P<0.05). در آزمون‌های مختلف تولید سورفکتانت زیستی، جدایه NW2کارایی بالایی از خود نشان داد. در مرحله بعد، شناسایی فنوتیپی جدایه‌های NW1،NW2 و NW7 انجام شد که نشان داد این باکتری‌ها به‌ترتیب متعلق به جنس‌های Pseudomonas، Bacillus و Rhodococcus هستند. همچنین، در آزمایش‌ خاک، این باکتری‌ها توانستند میزان فسفر قابل جذب خاک را به‌طور چشمگیری از 4/3 میلی‌گرم بر کیلوگرم به 46/8 میلی‌گرم بر کیلوگرم افزایش دهند (P<0.05). در نتیجه، یافته‌های این پژوهش بر پتانسیل زیست‌پالایی و افزایش حاصلخیزی خاک مبتنی بر میکروب‌های توانمند به‌عنوان یک راه حل مؤثر و پایدار برای پاکسازی مکان‌های آلوده به ترکیبات نفتی و انحلال فسفات نامحلول خاک، تأکید می‌کند.
کلیدواژه‌ها
انحلال فسفات؛ سورفکتانت زیستی؛ زیست‌پالایی؛ گل حفاری؛ نفت خام
عنوان مقاله [English]
The efficiency of oil-degrading and phosphate-solubilizing bacteria in phosphorus availability of a oil-contaminated calcareous soil
نویسندگان [English]
Komeil Zeynali1؛ Shayan Shariati2؛ Ahmad Ali Pourbabaee3؛ Mehdi Shorafa3
1Department of Soil Science Engineering, College of Agriculture &amp; Natural Resources, University of Tehran, Karaj, Iran
2Department of Environmental Engineering, Faculty of Environment, University of Tehran, Tehran, Iran.
3Department of Soil Science Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]
Abstract

Background and Objectives: The leakage of petroleum compounds into ecosystems poses a significant threat to the health of living organisms and humans. Various physical and chemical methods are employed to address oil contamination in soils; however, many of these methods are underutilized due to high costs and adverse environmental impacts. Physical methods are cost-effective but often fail to completely remove contaminants, leading to phase changes in pollutants. Chemical methods, such as precipitation, chemical oxidation, and the use of Fenton's reagent, are costly and environmentally unfriendly. Bioremediation, on the other hand, is widely used for the treatment of petroleum-contaminated soils due to its cost-effectiveness and environmental compatibility. This method relies on microorganisms to enzymatically degrade petroleum compounds into water and carbon dioxide. A key challenge in bioremediation is the limited availability of essential nutrients such as phosphorus in contaminated soils. Studies have shown that oil contamination significantly reduces the bioavailable phosphorus in soil, which acts as a critical limiting factor for plant growth and the effectiveness of bioremediation. To address this phosphorus deficiency, phosphate-solubilizing bacteria (PSBs) can be used to solubilize inorganic phosphate in the soil. This study aimed to isolate microorganisms capable of degrading crude oil and solubilizing phosphate from drilling mud and evaluate the changes in soil phosphorus after inoculation with these bacteria.

Materials and Methods: To enhance bioremediation and increase soil phosphorus availability, bacterial strains were initially isolated via enrichment techniques from drilling mud contaminated with high levels of total petroleum hydrocarbons (TPH). Their ability to utilize crude oil as the sole carbon and energy source was evaluated through three assays: growth on mineral salt medium (MSM) agar, growth in MSM, and crude oil degradation in MSM using a gravimetric method. Then, phosphate solubilization capability was determined using the spot-on-plate method in Sperber-agar, employing tricalcium phosphate [Ca3(PO4)2] as the insoluble phosphate source. Next, biosurfactant production was assessed using oil spreading, surface tension, and emulsification index (E24) assays, with crude oil as the carbon source. The isolates were phenotypically identified, and a soil microcosm experiment was designed using a completely randomized design with two treatments and six replicates in glass containers containing 300 grams of contaminated soil finally. The three top-performing isolates were mixed in equal proportions and inoculated into contaminated soil. After 30 days of incubation at 27°C, the available phosphorus content in the soil was measured using the sodium bicarbonate extraction method on days 0 and 30. Data variance analysis and graphing were performed using Minitab and Excel software, respectively.

Results: A total of seven distinct bacterial isolates were obtained from contaminated drilling mud at the end of enrichment stage. Crude oil degradation assays in MSM showed that isolates NW1, NW5, and NW7 achieved degradation rates of 20.6%, 19.66%, and 15.53%, respectively, and isolates NW2, NW7, and NW4 exhibited growth rates (OD₆₀₀) of 0.35, 0.35, and 0.31, respectively, over seven days. Phosphate solubilization tests revealed that isolates NW7 and NW5 demonstrated the highest solubilization indices of 3.76 and 2.83 on Sperber-agar, respectively (P<0.05). Based on these results, isolates NW1, NW2, and NW7 were selected for further experiments. In biosurfactant production tests, isolate NW2 exhibited the largest oil spread zone (1.26 cm) and the highest emulsification index (46.7%), significantly outperforming other isolates. The cumulative results indicated that NW2 was the most effective biosurfactant producer. Phenotypic identification classified NW1, NW2, and NW7 as belonging to the genera Pseudomonas, Bacillus, and Rhodococcus, respectively. The bacterial consortium significantly increased soil-available phosphorus from 3.4 mg/kg to 8.46 mg/kg compared to the control (P<0.05) in the soil microcosm experiment.

Conclusion: Oil compound spills have detrimental effects on ecosystems and human health, and conventional remediation methods face cost and environmental limitations. Bioremediation provides a sustainable and economical solution by employing microorganisms to degrade petroleum hydrocarbons. However, nutrient deficiencies, especially phosphorus, in contaminated soils restrict its efficiency. This study aims to isolate and evaluate oil-degrading and phosphate-solubilizing microorganisms from contaminated drilling mud, with the aim of increasing bioremediation efficiency and soil phosphorus levels. Among the seven isolated bacteria, NW1, NW2, and NW7 showed superior hydrocarbon degradation and phosphate solubilization capabilities and were identified as Pseudomonas, Bacillus, and Rhodococcus, respectively. The inoculation of these strains significantly enhanced soil available phosphorus, highlighting their potential for improving bioremediation efficiency and soil fertility. It is recommended that future studies investigate the phosphorus solubilization capability of these isolates in soils with varying levels of salinity, pH, and total phosphorus. Additionally, the impact of bacterial immobilization on different carriers for phosphorus solubilization in soil represents an interesting topic that has not yet been explored.
کلیدواژه‌ها [English]
Bioremediation, Biosurfactant, Crude oil, Drilling mud, Phosphate solubilization
مراجع
  1. Alexander, M. (1983). Most probable number method for microbial populations. Methods of Soil Analysis: Part 2 Chemical and Microbiological Properties, 9, 815-820.
  2. Aliyat, F. Z., Maldani, M., El Guilli, M., Nassiri, L., & Ibijbijen, J. (2020). Isolation and characterization of phosphate solubilizing bacteria from phosphate solid sludge of the moroccan phosphate mines. The Open Agriculture Journal, 14(1).
  3. Asemoloye, M. D., Jonathan, S. G., & Ahmad, R. (2019). Degradation of 2, 2-Dichlorovinyl dimethyl phosphate (dichlorvos) through the rhizosphere interaction between Panicum maximum Jacq and some selected fungi. Chemosphere, 221, 403-411.
  4. Behera, I. D., Basak, G., Kumar, R. R., Sen, R., & Meikap, B. C. (2020). Treatment of petroleum refinery sludge by petroleum degrading bacterium Stenotrophomonas pavanii IRB19 as an efficient novel technology. Journal of Environmental Science and Health, Part A, 56(2), 226-239.
  5. Bilen Ozyurek, S., & Seyis Bilkay, I. (2020). Comparison of petroleum biodegradation efficiencies of three different bacterial consortia determined in petroleum-contaminated waste mud pit. SN Applied Sciences, 2, 1-12.
  6. Black, C. A. (1965). Method of soil analysis part 2. Chemical and microbiological properties, 9, 1387-1388.
  7. Bouyoucos, G. J. (1962). Hydrometer method improved for making particle size analyses of soils 1. Agronomy journal, 54(5), 464-465.
  8. Cerqueda-García, D., García-Maldonado, J. Q., Aguirre-Macedo, L., & García-Cruz, U. (2020). A succession of marine bacterial communities in batch reactor experiments during the degradation of five different petroleum types. Marine Pollution Bulletin, 150, 110775.
  9. Chebbi, A., Hentati, D., Zaghden, H., Baccar, N., Rezgui, F., Chalbi, M., ... & Chamkha, M. (2017). Polycyclic aromatic hydrocarbon degradation and biosurfactant production by a newly isolated Pseudomonas strain from used motor oil-contaminated soil. International Biodeterioration & Biodegradation, 122, 128-140.
  10. Chen, W., Kong, Y., Li, J., Sun, Y., Min, J., & Hu, X. (2020). Enhanced biodegradation of crude oil by constructed bacterial consortium comprising salt-tolerant petroleum degraders and biosurfactant producers. International Biodeterioration & Biodegradation, 154, 105047.
  11. Chunyan, X., Qaria, M. A., Qi, X., & Daochen, Z. (2023). The role of microorganisms in petroleum degradation: Current development and prospects. Science of the Total Environment, 865, 161112.
  12. Gainer, A., Bresee, K., Hogan, N., & Siciliano, S. D. (2019). Advancing soil ecological risk assessments for petroleum hydrocarbon contaminated soils in Canada: Persistence, organic carbon normalization and relevance of species assemblages. Science of the total environment, 668, 400-410.
  13. Garrity, G. (2007). Bergey's manual of systematic bacteriology: volume 2: the Proteobacteria, part B: the Gammaproteobacteria (Vol. 2). Springer Science & Business Media.
  14. Girigiri, B., Ariole, C. N., & Stanley, H. O. (2019). Bioremediation of crude oil polluted soil using biofertilizer from nitrogen-fixing and phosphate solubilizing bacteria. American Journal of Nanosciences, 5(4): 27-38.
  15. Gyasi, S. F., Sarfo, M. K., Kabo-Bah, A. T., Adu, B., Appiah, A. S., & Serfor-Armah, Y. (2024). In vitro assessment of crude oil degradation by Acinetobacter junii and Alcanivorax xenomutans isolated from the coast of Ghana. Heliyon, 10(3).
  16. Hoang, S. A., Lamb, D., Sarkar, B., Seshadri, B., Yu, R. M. K., Tran, T. K. A., ... & Bolan, N. S. (2022). Phosphorus application enhances alkane hydroxylase gene abundance in the rhizosphere of wild plants grown in petroleum-hydrocarbon-contaminated soil. Environmental Research, 204, 111924.
  17. Hossain, M. F., Akter, M. A., Sohan, M. S. R., Sultana, N., Reza, M. A., & Hoque, K. M. F. (2022). Bioremediation potential of hydrocarbon degrading bacteria: isolation, characterization, and assessment. Saudi Journal of Biological Sciences, 29(1), 211-216.
  18. Huang, Y., Zhou, H., Zheng, G., Li, Y., Xie, Q., You, S., & Zhang, C. (2020). Isolation and characterization of biosurfactant-producing Serratia marcescens ZCF25 from oil sludge and application to bioremediation. Environmental Science and Pollution Research, 27, 27762-27772.
  19. Joe, M. M., Gomathi, R., Benson, A., Shalini, D., Rengasamy, P., Henry, A. J., ... & Sa, T. (2019). Simultaneous application of biosurfactant and bioaugmentation with rhamnolipid-producing shewanella for enhanced bioremediation of oil-polluted soil. Applied Sciences, 9(18), 3773.
  20. Kim, K. H., Jahan, S. A., Kabir, E., & Brown, R. J. (2013). A review of airborne polycyclic aromatic hydrocarbons (PAHs) and their human health effects. Environment International, 60, 71-80.
  21. Koohkan, H., Mortazavi, M. S., Golchin, A., Saraji, F., & Akbarzadeh-Chomachaei, G. (2023). Comparison of native bacterial and fungal bioaugmentation in the removal of petroleum from soil in the presence of sorghum. Water, Air, & Soil Pollution, 234(5), 309.
  22. Kumar, A. G., Manisha, D., Rajan, N. N., Sujitha, K., Peter, D. M., Kirubagaran, R., & Dharani, G. (2023). Biodegradation of phenanthrene by piezotolerant Bacillus subtilis EB1 and genomic insights for bioremediation. Marine Pollution Bulletin, 194, 115151.
  23. Lee, Y., Jeong, S. E., Hur, M., Ko, S., & Jeon, C. O. (2018). Construction and evaluation of a Korean native microbial consortium for the bioremediation of diesel fuel-contaminated soil in Korea. Frontiers in microbiology, 9, 2594.
  24. Li, H. P., Han, Q. Q., Liu, Q. M., Gan, Y. N., Rensing, C., Rivera, W. L., ... & Zhang, J. L. (2023). Roles of phosphate-solubilizing bacteria in mediating soil legacy phosphorus availability. Microbiological Research, 272, 127375.
  25. Li, X., Du, Y., Wu, G., Li, Z., Li, H., & Sui, H. (2012). Solvent extraction for heavy crude oil removal from contaminated soils. Chemosphere, 88(2), 245-249.
  26. Li, Z., Rosenzweig, R., Chen, F., Qin, J., Li, T., Han, J., ... & Ronen, Z. (2022). Bioremediation of petroleum-contaminated soils with biosurfactant-producing degraders isolated from the native desert soils. Microorganisms, 10(11), 2267.
  27. Madigan, M.T., Clark, D.P., Stahl, D. and Martinko, J.M., 2010. Brock biology of microorganisms 13th edition. Benjamin Cummings.
  28. Mahjoubi, M., Cappello, S., Souissi, Y., Jaouani, A., & Cherif, A. (2018). Microbial bioremediation of petroleum hydrocarbon–contaminated marine environments. Recent insights in petroleum science and engineering, 325, 325-350.
  29. Mirsaeidi, A. (2023). The effects of nitrogen and phosphorus nutrients on the bioremediation of oil-contaminated waters by Gracilariopsis persica in the coastal areas of Bandar Abbas. Marine Pollution Bulletin, 188, 114660.
  30. Mohammed, S. A., Omar, T. J., & Hasan, A. H. (2023). Degradation of crude oil and pure hydrocarbon fractions by some wild bacterial and fungal species. arXiv preprint arXiv:2301.08715.
  31. Mukred, A. M., Abd Hamid, A., Hamzah, A., & Yusoff, W. M. W. (2008). Growth Enhancement of Effective Microorganisms for Bioremediation of. Pakistan Journal of Biological Sciences, 11(13), 1708-1712.
  32. Muthukumar, B., Surya, S., Sivakumar, K., AlSalhi, M. S., Rao, T. N., Devanesan, S., ... & Rajasekar, A. (2023). Influence of bioaugmentation in crude oil contaminated soil by Pseudomonas species on the removal of total petroleum hydrocarbon. Chemosphere, 310, 136826.
  33. Nelson, D. A., & Sommers, L. (1983). Total carbon, organic carbon, and organic matter. Methods of soil analysis: Part 2 chemical and microbiological properties, 9, 539-579.
  34. Nikitha, T., Satyaprakash, M., Vani, S. S., Sadhana, B., & Padal, S. B. (2017). A review on polycyclic aromatic hydrocarbons: their transport, fate and biodegradation in the environment. International Journal of Current Microbiology and Applied Sciences, 6(4), 1627-1639.
  35. Okafor, U. C. (2023). Evaluation of the Impact of Crude Oil Contamination on Soil's Physicochemical Characteristics, Micro-flora and Crop Yield. Tropical Aquatic and soil pollution, 3(1), 24-35.
  36. Olajide, P. O., & Adeloye, A. O. (2023). Hydrocarbon biodegradation by Proteus and Serratia strains isolated from oil-polluted water in Bonny Community, Niger Delta, Nigeria. Results in Chemistry, 5, 100735.
  37. Olsen, S. R. (1954). Estimation of available phosphorus in soils by extraction with sodium bicarbonate (No. 939). US Department of Agriculture.
  38. Ou, Y., Wu, M., Yu, Y., Liu, Z., Zhang, T., & Zhang, X. (2024). Low dose phosphorus supplementation is conducive to remediation of heavily petroleum-contaminated soil—From the perspective of hydrocarbon removal and ecotoxicity risk control. Science of The Total Environment, 929, 172478.
  39. Owen, D., Williams, A. P., Griffith, G. W., & Withers, P. J. (2015). Use of commercial bio-inoculants to increase agricultural production through improved phosphrous acquisition. Applied Soil Ecology, 86, 41-54.
  40. Pahalvi, H. N., Rafiya, L., Rashid, S., Nisar, B., & Kamili, A. N. (2021). Chemical fertilizers and their impact on soil health. Microbiota and Biofertilizers, Vol 2: Ecofriendly tools for reclamation of degraded soil environs, 1-20.
  41. Partovinia, A., Soorki, A. A., & Koosha, M. (2021). Synergistic adsorption and biodegradation of heavy crude oil by a novel hybrid matrix containing immobilized Bacillus licheniformis: Aqueous phase and soil bioremediation. Ecotoxicology and Environmental Safety, 222, 112505.
  42. Phulpoto, I. A., Yu, Z., Li, J., Ndayisenga, F., Hu, B., Qazi, M. A., & Yang, X. (2022). Evaluation of di-rhamnolipid biosurfactants production by a novel Pseudomonas sp. S1WB: Optimization, characterization and effect on petroleum-hydrocarbon degradation. Ecotoxicology and Environmental Safety, 242, 113892.
  43. Quigg, A., Parsons, M., Bargu, S., Ozhan, K., Daly, K. L., Chakraborty, S., ... & Buskey, E. J. (2021). Marine phytoplankton responses to oil and dispersant exposures: Knowledge gained since the Deepwater Horizon oil spill. Marine Pollution Bulletin, 164, 112074.
  44. Ramirez, M. I., Arevalo, A. P., Sotomayor, S., & Bailon-Moscoso, N. (2017). Contamination by oil crude extraction–Refinement and their effects on human health. Environmental Pollution, 231, 415-425.
  45. Rayment, G. E., & Higginson, F. R. (1992). Australian Laboratory Handbook of Soil and Water Chemical Methods. Inkata Press Pty Ltd.
  46. Sattar, S., Siddiqui, S., Shahzad, A., Bano, A., Naeem, M., Hussain, R., ... & Yasmin, H. (2022). Comparative Analysis of Microbial Consortiums and Nanoparticles for Rehabilitating Petroleum Waste Contaminated Soils. Molecules, 27(6), 1945.
  47. Sayed, K., Baloo, L., & Sharma, N. K. (2021). Bioremediation of total petroleum hydrocarbons (TPH) by bioaugmentation and biostimulation in water with floating oil spill containment booms as bioreactor basin. International Journal of Environmental Research and Public Health, 18(5), 2226.
  48. Schryer, A. D., & Siciliano, S. D. (2024). Do phosphorus amendments enhance biodegradation activity in stalled petroleum hydrocarbon-contaminated soil? Journal of environmental quality, 10.1002/jeq2.20594.
  49. Shariati, S., Alikhani, H. A., Pourbabaee, A., & Mohammadi, L. (2013). The potential of application of different organic and inorganic carriers in insoluble phosphate solubilizing bacteria (Pseudomonas fluorescens) inoculants production process. International Journal of Agriculture: Research and Review, 3(1), 176. (In persian).
  50. Shariati, S., Ebenau-Jehle, C., Pourbabaee, A. A., Alikhani, H. A., Rodriguez-Franco, M., Agne, M., ... & Boll, M. (2021). Degradation of dibutyl phthalate by Paenarthrobacter sp. Shss isolated from Saravan landfill, Hyrcanian Forests, Iran. Biodegradation, 1-12.
  51. Sozina, I. D., & Danilov, A. S. (2023). Microbiological remediation of oil-contaminated soils. Journal of Mining Institute. 26,0 297-312.
  52. Sperber, J. I. (1958). Solution of apatite by soil microorganisms producing organic acids. Australian Journal of Agricultural Research, 9(6), 782-787.
  53. Tang, J., Lu, X., Sun, Q., & Zhu, W. (2012). Aging effect of petroleum hydrocarbons in soil under different attenuation conditions. Agriculture, Ecosystems & Environment, 149, 109-117.
  54. Tripathi, V., Gaur, V. K., Thakur, R. S., Patel, D. K., & Manickam, N. (2023). Assessing the half-life and degradation kinetics of aliphatic and aromatic hydrocarbons by bacteria isolated from crude oil contaminated soil. Chemosphere, 337, 139264.
  55. Varjani, S., & Upasani, V. N. (2019). Evaluation of rhamnolipid production by a halotolerant novel strain of Pseudomonas aeruginosa. Bioresource technology, 288, 121577.
  56. Viramontes-Ramos, S., Portillo-Ruiz, M. C., Ballinas-Casarrubias, M. D. L., Torres-Muñoz, J. V., Rivera-Chavira, B. E., & Nevárez-Moorillón, G. V. (2010). Selection of biosurfactan/bioemulsifier-producing bacteria from hydrocarbon-contaminated soil. Brazilian Journal of Microbiology, 41, 668-675.
  57. Wang, D., Lin, J., Lin, J., Wang, W., & Li, S. (2019). Biodegradation of petroleum hydrocarbons by Bacillus subtilis BL-27, a strain with weak hydrophobicity. Molecules, 24(17), 3021.
  58. Wang, S., Wang, D., Yu, Z., Dong, X., Liu, S., Cui, H., & Sun, B. (2021). Advances in research on petroleum biodegradability in soil. Environmental Science: Processes & Impacts, 23(1), 9-27.
  59. Xia, M., Liu, Y., Taylor, A. A., Fu, D., Khan, A. R., & Terry, N. (2017). Crude oil depletion by bacterial strains isolated from a petroleum hydrocarbon impacted solid waste management site in California. International Biodeterioration & Biodegradation, 123, 70-77.
  60. Xu, X., Liu, W., Tian, S., Wang, W., Qi, Q., Jiang, P., ... & Yu, H. (2018). Petroleum hydrocarbon-degrading bacteria for the remediation of oil pollution under aerobic conditions: a perspective analysis. Frontiers in microbiology, 9, 2885.
  61. Zeynali, K., Shariati, S., & Pourbabaee, A. A. (2024a). The role of effective oil-eating bacteria in the remediation of oil-contaminated soils (Case study: Bacillus genus). Journal of Sol Biology, 12(1), 105-139. (In persian).
  62. Zeynali, K., Shariati, S., Pourbabaei, A. A., & Shorafa, M. (2024b). The application of biosurfactant producing bacterial consortium as a petroleum degrader in increasing the hydraulic conductivity coefficient of TPH-contaminated soil. Iranian Journal of Soil and Water Research, 1585-1599. (In persian)
  63. Žvirgždas, J., Paškevičius, A., Petrovas, S., Galginienė, I., & Iljasevičius, K. (2023). Isolation, selection, and use of oil-degrading microorganisms for biological treatment of contaminated soil. Polish Journal of Environmental Studies, 32(3), 1-10.
 

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