Heterogeneity of pathogenicity of Ralstonia solanacearum strains isolated from potato fields in Hamadan province | ||
| مهار زیستی در گیاهپزشکی | ||
| Volume 11, Issue 1, August 2023, Pages 135-148 PDF (1.09 M) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/bcpp.2024.365942.367 | ||
| Authors | ||
| negin taheri1; Gholam Gholam Khodakaramian* 2 | ||
| 1M. Sc. student,, Department of Plant Protection, Faculty of Agriculture, Bu– Ali Sina University, Hamedan, Iran. | ||
| 2Professor, Department of Plant Protection, Faculty of Agriculture, Bu– Ali Sina University, Hamedan, Iran. | ||
| Abstract | ||
| Abstract One of the most devastating potato diseases in Hamadan province is bacterial wilt caused by Ralstonia solanacearum. The production of extracellular polysaccharide is the most important pathogenic factor of this bacterium. To investigate the heterogeneity of pathogenicity among pathogenic strains of bacteria, plant samples suspected of contamination were collected from the potato growing areas of the province. In the laboratory, bacterial strains of R. solanacearum were isolated from infected potato samples on Nutrient Agar (NA) containing tri–phenyl tetrazolium chloride (TTC). Screening of isolated bacteria was done based on colony appearance on TTC culture medium, hypersensitivity reaction on tobacco & tyrosinase activity. A total of 63 bacterial strains were selected & their critical phenotype were characterized. To select representatives of bacterial strains for greenhouse experiment, hypersensitivity reaction on tobacco and pathogenicity on potato and geranium were investigated for all strains. The pattern of bacterial cell soluble proteins electrophoresed in polyacrylamide gel was investigated. Results showed that most of the strains showed hypersensitive reaction on tobacco & they were pathogenic on potato & geranium. In a separate experiment, bacterial strains were grouped based on the severity of pathogenicity on geranium leaves & the obtained data were analyzed with SAS software. Finally, based on the appearance & phenotypic characteristics of bacterial strains, the pattern of electrophoresed cell soluble proteins, pathogenicity in geranium & hypersensitivity reaction on tobacco, 10 representative strains were selected to investigate the heterogeneity of pathogenicity on potato and tomato in greenhouse. In greenhouse conditions, the variation of pathogenicity among the selected strains on potato & tomato was investigated in a randomized complete block design with three replicates. Results showed that the tested strains were heterogeneous in terms of pathogenicity in both potato & tomato plants and showed significant differences. In some cases, tested strains were up to five statistical groups based on the effect on plant growth factors such as epinasty, plant total weight, wet and dry weight of upper parts & roots. This important finding can clarify our point of view on the control of bacterial wilt disease of these two very important products based on the use of resistant or tolerant cultivars & integrated control and open a new window for us to efficient control of the disease. . | ||
| Keywords | ||
| Key words: Epinasty; R. solanacearum bacterial wilt of tomato; bacterial wilt of potato; Geranium | ||
| References | ||
|
Agrios, G.N. 2005. “Plant Phathology”. Elsevier Academic Prss, PP.922.
Ahmed, N.N., Islam, M.R., Hossain, M.A., Meah, M.B. & Hossain, M.M. 2013. Determination of races and biovars of Ralstonia solanacearum causing bacterial wilt disease of potato. Journal of Agricultural Science, 5(6): 86.
Bahar, M. & Danesh, D. 2017. "Etiology of potato bacterial wilt in Iran". Plant Diseases, 24: 1–12.
Fahy, P.C. & Hayward, A.C. 1983. Media & methods for isolation & diagnostic tests. In Klement, Z., Rudolph, K., Stands, D., C. (eds)."Methods in Phytobacteriology." Akademiai Kiado, Budapest, P. 20–28. Microbiology, 2: 131–141.
Gutarra, L., Herrera, J., Fernandez, E., Kreuze, J. & Lindqvist–Kreuze, H. 2017. Diversity, pathogenicity and current occurrence of bacterial wilt bacterium Ralstonia solanacearum in Peru. Frontiers in Plant Science, 8: 1221.
Hayward, A.C. & Hartman, G.L. 1991. Bacterial wilt the disease and its causative agent Pseudomonas solanacearum. Wallingford, UK: CAB International in Association with AVRDC.
Hong, J.C., Norman, D.J., Reed, D.L., Momol, M.T. & Jones, J.B. 2012. Diversity among Ralstonia solanacearum strains isolated from the southeastern United States. Phytopathology, 102: 924–936.
Horita, M. & Tsuchiya, K. 2000. “Genetic Diversity of Japanese Strains of Ralstonia solanacearum”. The American Phytopathological Society, 91(4): 399–407.
Hosseinzadeh, S., Falahi Charkhabi, N., Rastgo, M. & Hosseinzadeh, A. 2014. "Plant Bacteriology" in Agricultural Education & Extension. Page 346.
Huang, Q. & Allen, C. 1997. "An exo–poly–a–D–galacturonosidase, PehB, is requred for wild–type virulence of Ralstonia solanacearum". Journal of Bacteriology, 179: 7369–7378.
Janse, J.D. 2012. Review on brown rot (Ralstonia solanacearum race 3, biovar 2, phylotype IIB) epidemiology and control in the Netherlands since 1995: a success story of integrated pest management. Journal of Plant Pathology, 257–272.
Kao, C.C. & Sequeira, L. 1991. "A gene cluster required for coordinated biosynthesis of lipopolysaccharide and extracellular polysaccharide also affects virulence of Pseudomonas solanacearum". Journal of Bacteriology, 173: 7841–7848.
Klement, Z., Rudolph, K. & Sands, D.C. 1990. Methods in phytobacteriology. Akadémiai Kiadó.
Khairy, A.M., Tohamy, M.R., Zayed, M.A. & Ali, M.A. 2021. Detecting pathogenic bacterial wilt disease of potato using biochemical markers and evaluate resistant in some cultivars. Saudi Journal of Biological Sciences, 28(9): 5193–5203.
Kiba, A., Maimbo, M., Kanda, A., Tomiyama, H., Ohnishi, K. & Hikichi, Y. 2007. Isolation and expression analysis of candidate genes related to Ralstonia solanacearum–tobacco interaction. Plant Biotechnology, 24(4): 409–416.
Li, Y., Feng, J., Liu, H., Wang, L., Hsiang, T., Li, X. & Huang, J. 2016. Genetic diversity & pathogenicity of Ralstonia solanacearum causing tobacco bacterial wilt in China. Plant Disease, 100(7): 1288–1296.
Muthoni, J., Shimelis, H., Melis, R. & Kinyua, Z.M. 2014. Response of potato genotypes to bacterial wilt caused by Ralstonia solanacearum (Smith) (Yabuuchi et al.) in the tropical highlands. American Journal of Potato Research, 91(2): 215–232.
Schaad, N.W., Jones, J.B. & Chun, W. 2001. "Laboratory Guide for Identification of Plant Pathogenic Bacteria". APS Press, St. Pual, Minnesota, 373 pp.
Yabuuchi, E., Kosako, Y., Yano, I., Hotta, H. & Nishiuchi, Y. 1995. Transfer of two Burkholderia & an Alcaligenes species to Ralstonia gen. nov.: proposal of Ralstonia pickettii (Ralston, Palleroni and Doudoroff 1973) comb. nov., Ralstonia solanacearum (Smith 1896) comb. nov. and Ralstonia eutropha (Davis1969) comb. nov. Microbiology and Immunology, 39(11): 897–904.
| ||
|
Statistics Article View: 342 PDF Download: 316 |
||
Journal management system. designed by sinaweb