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Strain improvement of Trichoderma afroharzianum through induced gamma radiation mutation for cellulase and xylanase production enhancement | ||
| Mycologia Iranica | ||
| دوره 11، شماره 1، شهریور 2024، صفحه 77-92 اصل مقاله (710.33 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22092/mi.2024.365994.1283 | ||
| نویسندگان | ||
| Hamed Askari1؛ Samira Shahbazi* 1؛ Sabihe Soleimanian-Zad2؛ Mahdi Kadivar3 | ||
| 1Nuclear Agriculture School, Nuclear Science and Technology Research Institute (NSTRI), Atomic Energy Organization of Iran (AEOI), Karaj, Iran | ||
| 2Department of Food Science and Technology, College of Agriculture, Isfahan University of Technology, Isfahan, 84156-83111, Iran Research Institute for Biotechnology and Bioengineering, Isfahan University of Technology, Isfahan, 84156-83111, Iran | ||
| 3Department of Food Science and Technology, College of Agriculture, Isfahan University of Technology, Isfahan, 84156-83111, Iran | ||
| چکیده | ||
| Cellulases and xylanases are hydrolytic enzymes that randomly cleave the β-1, 4 backbones of the cellulose and arabinoxylans of wheat flour and are widely used in the bakery industry as a dough texture improver in the formulations of flour products. Creating novel sources of a microbial strain using induced gamma irradiation can increase enzyme production for bakery industrial usage. According to this, Co60 gamma irradiation has been used to develop a mutant strain of Trichoderma afroharzianum. Trichoderma mutants were isolated, and the qualitative and quantitative screening were used to evaluate the extracellular enzyme production with the wheat bran waste as a substrate. The best Trichoderma mutant isolate was identified using the DNA barcoding method. The highest xylanase activities were observed in the superior mutant isolate of Trichoderma afroharzianum NAS107-M82, which is approximately 3.3 times higher than its parent strain. The electrophoretic pattern of proteins showed that the exo-glucanase I, endo-glucanase III, and the xylanase I enzymes hydrolyzed the wheat bran, synergistically. Overall, gamma irradiation-induced mutation could be an expedient technique to access such superior mutants for the bioconversion of wheat bran wastes to xylanase enzyme. | ||
| کلیدواژهها | ||
| Trichoderma؛ Wheat bran؛ Cellulase-Xylanase؛ Gamma radiation؛ Mutation | ||
| مراجع | ||
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