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Afiat, Z., Norozpoor, M., Sarikhani, M., Aliasgharzad, N. (2024). Monitoring of Dehydrogenase and Lipase Activity Changes in a Light Naphtha-Contaminated Soil under Different Bioremediation Treatments. , 38(1), 43-59. doi: 10.22092/ijsr.2024.362387.706
Zakieh Afiat; Maryam Norozpoor; Mohammad Reza Sarikhani; Nasser Aliasgharzad. "Monitoring of Dehydrogenase and Lipase Activity Changes in a Light Naphtha-Contaminated Soil under Different Bioremediation Treatments". , 38, 1, 2024, 43-59. doi: 10.22092/ijsr.2024.362387.706
Afiat, Z., Norozpoor, M., Sarikhani, M., Aliasgharzad, N. (2024). 'Monitoring of Dehydrogenase and Lipase Activity Changes in a Light Naphtha-Contaminated Soil under Different Bioremediation Treatments', , 38(1), pp. 43-59. doi: 10.22092/ijsr.2024.362387.706
Afiat, Z., Norozpoor, M., Sarikhani, M., Aliasgharzad, N. Monitoring of Dehydrogenase and Lipase Activity Changes in a Light Naphtha-Contaminated Soil under Different Bioremediation Treatments. , 2024; 38(1): 43-59. doi: 10.22092/ijsr.2024.362387.706

Monitoring of Dehydrogenase and Lipase Activity Changes in a Light Naphtha-Contaminated Soil under Different Bioremediation Treatments

پژوهش های خاک
Article 4, Volume 38, Issue 1, August 2024, Pages 43-59    PDF (1.48 M)
Document Type: Research Paper
DOI: 10.22092/ijsr.2024.362387.706
Authors
Zakieh Afiat1; Maryam Norozpoor1; Mohammad Reza Sarikhani* 2; Nasser Aliasgharzad3
1University of Tabriz, Department of Soil Science
2soil
3University of Tabriz
Abstract
Enzyme activity measurement is one of the significant methods in the bioremediation of soils contaminated with petroleum compounds. Biostimulation, bioaugmentation and their integration are among the types of bioremediation methods used in this experiment. For this purpose, to reduce light naphtha pollution (1%) in a sandy loam soil, a variety of bioremediation treatments, including providing NP elements, adding cow manure and Tween 80 surfactant, bioaugmentation, and integrated treatment were used. This experiment was carried out in a pot (containing 3 kg soil) based on split plot factorial design (pollution factor, bioremediation factor and time) with 3 replications, at room temperature, for 120 days. On days 0, 5, 10, 15, 30, 45, 60, 90 and 120, subsamples were taken from each pot to measure the activity of dehydrogenase and lipase enzymes. The results of the experiment showed that bioremediation treatments led to the reduction of oil pollutants. Likewise, dehydrogenase activity had a completely downward trend from the beginning of the experiment, and the changes of lipase activity in contaminated soil increased until day 10, decreased slightly from day 10 to 15, then, progressed with slight fluctuations until day 60 and decreased again from day 60 onward. Dehydrogenase activity changes in cow manure treatment during the experiment was from 3.39 to 0.35 (μg TPF g-1 h-1), and lipase activity in integrated treatment dropped from 57 to 33.56 (mU g-1). In addition to increasing the active microbial population involved in the decomposition of petroleum compounds and providing optimal conditions for their activity, the use of integrated treatments and cow manure is a suitable method to reduce the concentration of light naphtha.
Keywords
Bioaugmentation; Bioremediation; Biostimulation; Enzyme activity; Petroleum pollution
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