اخبار و اعلانات

 آمار

تعداد نشریات287
تعداد نشریات سازمان85
تعداد شماره‌ها3,159
تعداد مقالات35,108
تعداد مشاهده مقاله50,530,380
تعداد دریافت فایل اصل مقاله90,478,999
Esmaeili, Hossein, Ghasemifard, hassan, hamedi, mona, sharifan, mahyar, bahari, pejman. (1404). Prevalence and Identification of Infectious Abortion Pathogens in Sheep Flocks of North Khorasan, Iran. سامانه مدیریت نشریات علمی, 80(2), 431-435. doi: 10.32592/ARI.2025.80.2.431
Hossein Esmaeili; hassan Ghasemifard; mona hamedi; mahyar sharifan; pejman bahari. "Prevalence and Identification of Infectious Abortion Pathogens in Sheep Flocks of North Khorasan, Iran". سامانه مدیریت نشریات علمی, 80, 2, 1404, 431-435. doi: 10.32592/ARI.2025.80.2.431
Esmaeili, Hossein, Ghasemifard, hassan, hamedi, mona, sharifan, mahyar, bahari, pejman. (1404). 'Prevalence and Identification of Infectious Abortion Pathogens in Sheep Flocks of North Khorasan, Iran', سامانه مدیریت نشریات علمی, 80(2), pp. 431-435. doi: 10.32592/ARI.2025.80.2.431
Esmaeili, Hossein, Ghasemifard, hassan, hamedi, mona, sharifan, mahyar, bahari, pejman. Prevalence and Identification of Infectious Abortion Pathogens in Sheep Flocks of North Khorasan, Iran. سامانه مدیریت نشریات علمی, 1404; 80(2): 431-435. doi: 10.32592/ARI.2025.80.2.431

Prevalence and Identification of Infectious Abortion Pathogens in Sheep Flocks of North Khorasan, Iran

Archives of Razi Institute
مقاله 18، دوره 80، شماره 2، خرداد و تیر 2025، صفحه 431-435    اصل مقاله (365.18 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2025.80.2.431
نویسندگان
Hossein Esmaeili* 1؛ hassan Ghasemifard2؛ mona hamedi3؛ mahyar sharifan2؛ pejman bahari4
1of Microbiology, Faculty of Veterinary Medicine, University of Tehran
22Laboratory of North Khorasan Veterinary Head Office, Bojnurd, Iran
3Department of Immunopathology, Faculty of Veterinary Medicine, Islamic Azad University, Science and Research branch, Tehran, Iran.
4Laboratory of North Khorasan Veterinary Head Office, Bojnurd, Iran
چکیده
Abortion is one of the main causes of reproduction losses in small ruminant’s flocks in the world. Infection with the agents including Toxoplasma gondii, Campylobacter spp., Chlamydia abortus, and Coxiella burnetii frequently occurs worldwide. Brucella melitensis is the most important cause of abortion in Iran and its neighbors. Other abortifacient agents such as C. abortus and C. burnetii are prevalent among sheep flocks as well. The present study aimed to investigate the presence of the most common abortifacient pathogens in sheep in North Khorasan, Iran. The samples were collected from 133 aborted sheep fetuses. Then, using ELISA, conventional PCR and bacteriological examination the presence of pathogens including Escherichia coli, B. melitensis, Salmonella spp., C. burnetii, Campylobacter spp., Leptospira spp., Listeria monocytogenes, Toxoplasma gondii, Border disease virus and Blue Tongue virus were assessed. Using bacteriological culture, E. coli (9%) and B. melitensis (12%) were isolated. C. burnetii (2.5%), Toxoplasma gondii (12%), Border disease virus (3%) and Blue Tongue virus (9%) were identified in fetal serology. B. melitensis (12%), Salmonella (8.5%), Campylobacter spp. (1.7%), Leptospira spp. (2.5%), Chlamydia abortus (25.6%) C. burnetii (14.5%), and T. gondii (6.8%) were detected by PCR. C. abortus was the most frequent pathogen detected by PCR (25.6%). The present results showed the studied sheep flocks are infected with the most important abortifacient pathogens which emphasize the demand for more investigations for the detection of abortion causes based on the different geographical regions using simple and sensitive methods. Epidemiological and risk factors contribute in ovine abortion is further necessary.
کلیدواژه‌ها
Abortion؛ Brucellosis؛ Chlamydia abortus؛ Sheep؛ Small ruminant
سایر فایل های مرتبط با مقاله
مراجع
  1. Esmaeili H, Ghorani M, Baghal Arani E, Shakeri, AP. Detection of contagious ovine ecthyma (orf) and risk factors for infection in small ruminants in Iran. Comparative Immunology, Microbiology and Infectious Diseases. (2021b); 79:
  2. Agriculture Ministry. Annual report of farm animal products in Iran, ministry of agriculture. In: Aitken, I. Diseases of sheep. California: John Wiley & Sons; 2020. P. 4.
  3. Noakes DE, Parkinson TJ, England GC. Arthur's Veterinary Reproduction and Obstetrics. 8th ed. Amsterdam: Elsevier publishing; 2016.
  4. Esmaeili H, Tajik P, Ekhtiyarzadeh H, Bolourchi M, Hamedi M, Khalaj M, Amiri K. Control and eradication program for bovine brucellosis in Iran: an epidemiological survey. Journal of Veterinary Research. 2012b; 67: 211–221.
  5. Van Engelen E, Luttikholt S, Peperkamp K, Vellema P, Van Den Brom R. Small ruminant abortions in the Netherlands during lambing season 2012-2013. Veterinary Record. 2014; 174: 506.
  6. Bricker BJ, Halling SM. Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. Journal of Clinical Microbiology. 1994; 32: 2660–2666.
  7. Rahn K, De Grandis S, Clarke R, McEwen S, Galan J, Ginocchio C, Curtiss Iii R, Gyles C. Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of Salmonella. Molecular and Cellular Probes. 1992; 6: 271–279.
  8. Bang DD, Pedersen K. Madsen M. Development of a PCR assay suitable for Campylobacter Spp. mas screening programs in broiler production. Journal of rapid methods and automation in microbiology. 2001; 9: 97- 113.
  9. Laroucau K, Souriau A. Rodolakis A. Improved sensitivity of PCR for Chlamydophila using pmp genes. Veterinary microbiology. 2001; 82: 155- 164.
  10. Hoover T, Vodkin M, Williams J. A Coxiella burnetti repeated DNA element resembling a bacterial insertion sequence. Journal of Bacteriology. 1992; 174: 5540–5548.
  11. Burg JL, Grover CM, Pouletty P, Boothroyd JC. Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction. Journal of Clinical Microbiology. 1989; 27: 1787–1792.
  12. Gouws PA, Liedemann I. Evaluation of diagnostic PCR for the detection of Listeria monocytogenes in food products. Food Technology and Biotechnology. 2005; 43: 201–205.
  13. Stoddard RA, Gee JE, Wilkins PP, McCaustland K, Hoffmaster AR. Detection of pathogenic Leptospira spp. through TaqMan polymerase chain reaction targeting the LipL32 gene. Diagnostic Microbiology and Infectious Disease. 2009; 64 (3): 247-55.
  14. Longbottom D, Coulter L. Animal chlamydiosis and zoonotic implications. Journal of Comparative Pathology. 2003; 128 (4): 217-44.
  15. Esmaeili H, Shakeri A, nosrati Rad Z, Baghal Arani E, Villanueva-Saz S, Ruiz H, Lacasta D. Causes of abortion in Iranian sheep flocks and associated risk factors. Veterinary Research Communications. 2022; 46: 1227-1238.
  16. Kennedy HE, McCullough SJ, Graham D, Cassidy G, Malone FE, Ellis WA. Detection of Chlamydial antibody by fetal serology- an aid to the diagnosis of ovine abortion. Journal of Veterinary Diagnostic Investigation. 2001; 13: 30-35.
  17. Ghorani M, Esmaeili H. Comparison of susceptibility of different goat breeds to live attenuated goatpox vaccine. Small Ruminant Research. 2022; 212: 106721.
  18. Mostafavi E, Molaeipoor L, Esmaeili S, Ghasemi A, Kamalizad M, Yousefi Behzadi M, Naserifar R, Rohani M, Hashemi Shahraki A. Seroprevalence of Q fever among high-risk occupations in the Ilam province, the west of Iran. PLoS One. 2019; 14 (2): 1-10.
  19. Plummer PJ, McClure JT, Menzies P, Morley PS, Van den Brom R, Van Metre DC. Management of Coxiella burnetii infection in livestock populations and the associated zoonotic risk: a consensus statement. Journal of veterinary Internal Medicine. 2018; 32 (5): 1481-1494.
  20. Esmaeili H, Kalateh Rahmani H. Detection of Salmonella carriers in sheep and goat flocks of Bushehr and Lorestan provinces, Iran. Journal of Medical Bacteriology. 2016; 5: 50-53.
  21. Elvira Partida L, Fernández M, Gutiérrez J, Esnal A, Benavides J, Pérez V, Torre A, Alvarez M, Esperon F. Detection of bovine viral diarrhoea virus 2 as the cause of abortion outbreaks on commercial sheep flocks. Transboundary and Emerging Diseases. 2017; 64:19–26.
  22. Hazlett MJ, McDowall R, DeLay J, Stalker M, McEwen B, van Dreumel T, Spinato M, Binnington B, Slavic D, Carman S. A prospective study of sheep and goat abortion using real-time polymerase chain reaction and cut point estimation shows Coxiella burnetii and Chlamydophila abortus infection concurrently with other major pathogens. Journal of Veterinary Diagnostic Investigation. 2013; 25: 359–368.
  23. Gangneux FR, Darde ML. Epidemiology of and diagnostic strategies for toxoplasmosis. Clinical Microbiology reviews. 2012; 25 (2): 264-296.
  24. García-Pérez AL, Minguijon E, Estevez L, Barandika JF, Aduriz G, juste RA, Hurtada A. Clinical and laboratorial findings in pregnant ewes and their progeny infected with Border disease virus (BDV-4 genotype). Research in Veterinary Science. 2009; 86: 345-352.
  25. Rasooli A, Nouri M, Seyfi Abad Shapouri MR, Mohseni-Parsa S, Baghbanian HR, Lotfi M, Daghari M. Serological Detection of SRMV, BVDV, BHV-1and BEFV in Camels (Camelus dromedarius) in Southwest Iran. Iranian Journal of Veterinary Medicine. 2023; 17 (2): 139-148.
آمار
تعداد مشاهده مقاله: 956
تعداد دریافت فایل اصل مقاله: 676


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب