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Abhari, Abbas, Masoumi, Aali, Gholinezhad, Esmaeil, Azizi, Elham. (1403). Physiological Responses of Melissa the Effects of Growth Regulators on Induction of Callus Tissue and Regeneration of Harmala (Peganum harmala L.). سامانه مدیریت نشریات علمی, 13(3), 551-559. doi: 10.22034/jmpb.2023.362232.1557
Abbas Abhari; Aali Masoumi; Esmaeil Gholinezhad; Elham Azizi. "Physiological Responses of Melissa the Effects of Growth Regulators on Induction of Callus Tissue and Regeneration of Harmala (Peganum harmala L.)". سامانه مدیریت نشریات علمی, 13, 3, 1403, 551-559. doi: 10.22034/jmpb.2023.362232.1557
Abhari, Abbas, Masoumi, Aali, Gholinezhad, Esmaeil, Azizi, Elham. (1403). 'Physiological Responses of Melissa the Effects of Growth Regulators on Induction of Callus Tissue and Regeneration of Harmala (Peganum harmala L.)', سامانه مدیریت نشریات علمی, 13(3), pp. 551-559. doi: 10.22034/jmpb.2023.362232.1557
Abhari, Abbas, Masoumi, Aali, Gholinezhad, Esmaeil, Azizi, Elham. Physiological Responses of Melissa the Effects of Growth Regulators on Induction of Callus Tissue and Regeneration of Harmala (Peganum harmala L.). سامانه مدیریت نشریات علمی, 1403; 13(3): 551-559. doi: 10.22034/jmpb.2023.362232.1557

Physiological Responses of Melissa the Effects of Growth Regulators on Induction of Callus Tissue and Regeneration of Harmala (Peganum harmala L.)

Journal of Medicinal plants and By-products
مقاله 8، دوره 13، شماره 3، آذر 2024، صفحه 551-559    اصل مقاله (864.2 K)
نوع مقاله: Research Article
شناسه دیجیتال (DOI): 10.22034/jmpb.2023.362232.1557
نویسندگان
Abbas Abhari* 1؛ Aali Masoumi2؛ Esmaeil Gholinezhad2؛ Elham Azizi2
1Research Center for Geographical Sciences and Social Studies, Environmental Stress Studies Core, Hakim Sabzevari University, Iran
2Department of Agricultural Sciences, Payame Noor University, Tehran, Iran
چکیده
There has been limited information on the in-vitro propagation of Harmal. In this research, leaf, hypocotyl and embryo samples of this plant were examined for callus formation and regeneration. The present experiment was conducted as factorial based on a completely randomized design with three replications in two separate experiments with different hormonal treatments. In the first experiment, MS medium contained growth with different concentrations of Benzylaminopourine ((BAP) 0, 0.5, 1 and 2 mg/L) in combination with Naphthalene acetic acid (NAA) at concentrations (0, 0.25 and 0.5 mg/L. In the second experiment, BAP with concentrations (0, 0.5, 1 and 2 mg/L) in combination with 2,4-Dichlorophenoxyacetic acid (2, 4-D) with concentrations (0, 25 and 0.5 mg / L). The results of the first experiment showed that the highest callus and regeneration for the Harmal a plant was the combination of BAP hormone (0.5 mg/L) with NAA (0.5 mg/L). In this research, leaf, hypocotyl and embryo samples of this plant were examined for callus formation and regeneration. The results of the second experiment, on the other hand, showed that callus of explants was carried out in most of the BAP and 2,4-D hormonal treatments, and the highest regeneration was observed in the treatment of 1 mg/L BAP with 0.5 mg/L 2,4-D. High BAP concentration in the presence of NAA decreased callus formation and appeared to be better for NAA callus enhancement. The high concentration of BAP in the presence of NAA reduced callus formation and it can be said that NAA was better for increasing callus production. It was found that in the presence of 2,4-D and BAP, leaf explants were more efficient for regeneration and callus formation compared to hypocotyl and embryo explants. In the medium supplemented with 0.5 mg/L 2,4-D and 1 mg/L BA for the leaf and hypocotyl explants, Maximum callus induction and shoot regeneration was obtained.
کلیدواژه‌ها
BAP؛ Callus؛ Harmala؛ Regeneration؛ and 2؛ 4-D
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