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مهار زیستی بیمارگرهای مهم برنج با استفاده از قارچهای اندوفیت جداشده از برنج هوازی | ||
| مهار زیستی در گیاهپزشکی | ||
| مقاله 6، دوره 10، شماره 1، شهریور 1401، صفحه 107-125 اصل مقاله (886.04 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/bcpp.2023.361900.331 | ||
| نویسندگان | ||
| زینب زارع رحمت آباد1؛ سید اسماعیل رضوی* 2؛ کامران رهنما3؛ عبدالحسین طاهری4؛ جاوید قرخلو5 | ||
| 1دانشجوی دکتری، بیماریشناسی گیاهی، گروه گیاهپزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی منابع طبیعی گرگان | ||
| 2استادیار، گروه گیاهپزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی منابع طبیعی گرگان، ایران. | ||
| 3دانشیار، گروه گیاهپزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی منابع طبیعی گرگان، ایران. | ||
| 4دانشیار، گروه گیاهپزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی منابع طبیعی گرگان | ||
| 5دانشیار، گروه زراعت، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی منابع طبیعی گرگان | ||
| چکیده | ||
| برنج از مهمترین غلات مورد مصرف غذایی مردم جهان به ویژه خاورمیانه میباشد. بیماری بلاست و پوسیدگی طوقه و ریشه، دو بیماری قارچی مهم برنج در مناطق مختلف محسوب می شوند. قارچ های اندوفیت یکی از عوامل کنترل بیولوژیک محسوب شده که علاوه بر کنترل بیماری های گیاهی موجب بهبود رشد در گیاه میزبان میشوند. بدین منظور جدایه های قارچی اندوفیت و همچنین جدایه های بیمارگر از برگ، غلاف، ساقه و ریشه بوته های برنج هوازی از شالیزارهای استان گلستان جداسازی و شناسایی مورفولوژیکی و مولکولی شدند. سپس فعالیت آنتاگونیستی آن ها علیه جدایه بیمارگر Magnaporthe oryzae عامل بیماری بلاست و جدایه بیمارگر Fusarium fujikuroi عامل بیماری پوسیدگی طوقه و ریشه به سه روش، کشت متقابل، متابولیت های فرار و متابولیت های غیر فرار در آزمایشگاه بررسی شد. نتایج تحقیق نشان داد، در روش کشت متقابل جدایه F. incarnatum بیشترین میزان بازدارندگی از رشد قارچ بیمارگر M. oryzae و قارچ بیمارگر F. fujikuroi را به ترتیب با میانگین 58/64و 60/03درصد داشت. در روش متابولیت های فرار بیشترین درصد مهار قارچ بیمارگر F. fujikuroi را جدایه E. nigrum با 57/13درصد بازدارندگی و جدایه F. incarnatum با 52/24درصد بازدارندگی از خود نشان دادند. در روش متابولیت های غیر فرار (عصاره)، جدایه های F. incarnatum و F. proliferatum به ترتیب با میانگین 62/27و 57/96درصد، بیشترین درصد ممانعت از رشد قارچ عامل بیماری بلاست را از خود نشان دادند. جدایه F. incarnatum حاصل از این پژوهش فعالیت آنتاگونیستی را در سه آزمون کشت متقابل، متابولیت های فرار و متابولیت های غیر فرار علیه قارچهای M. oryzae و F. fujikuroi نشان داد. همچنین در این آزمایش جدایه های F. proliferatum و F. incarnatum در شرایط گلخانه موجب مهار بیماری بلاست و پوسیدگی طوقه و افزایش ارتفاع و وزن تر اندام هوایی و ریشه گیاه برنج شدند. | ||
| کلیدواژهها | ||
| Magnaporthe oryzae؛ متابولیتهای فرار؛ درصد بازدارندگی؛ F. incarnatum؛ اندوفیت برنج | ||
| عنوان مقاله [English] | ||
| Biological control of important rice pathogens using endophytic fungi isolated from aerobic rice | ||
| نویسندگان [English] | ||
| zeinab zare rahmat abad1؛ seyed esmail razavi2؛ kamran Rahnama3؛ Abdolhossein Taheri4؛ Javid Gherekhloo5 | ||
| 1Ph.D. student of plant pathology, Department of Plant Protection, Faculty of plant production, Gorgan University of Agricultural sciences and Natural Resources, Gorgan, Iran | ||
| 2Assistant Professor, Department of Plant Protection, Faculty of plant production, Gorgan University of Agricultural sciences and Natural Resources, Gorgan, Iran. | ||
| 3Associate professor, Department of Plant Protection, Faculty of plant production, Gorgan University of Agricultural sciences and Natural Resources, Gorgan | ||
| 4Associate professor, Department of Plant Protection, Faculty of plant production, Gorgan University of Agricultural sciences and Natural Resources, Gorgan | ||
| 5Associate professor, Department of Agriculture, Faculty of plant production, Gorgan University of Agricultural sciences and Natural Resources, Gorgan | ||
| چکیده [English] | ||
| Rice is most important grains consumed by the people of the world. Blast disease and crown and root rot are two important fungal diseases of rice. Endophytic fungi are biological control agents plant diseases and improve growth in the host plant. For this purpose, endophytic fungal isolates and pathogenic isolates from leaves, pods, stems and roots of aerobic rice plants of Golestan province were isolated and identified morphologically and molecularly. Then, their antagonistic activity against Magnaporthe oryzae and Fusarium fujikuroi disease were investigated by three methods, dual culture, volatile metabolites and non–volatile metabolites. The results showed that, in dual culture method, the F. incarnatum had the highest inhibition rate against the growth of M. oryzae and F. fujikuroi, with 58.64 and 60.03% inhibition, respectively. In volatile metabolites method, E. nigrum with 57.13% and F. incarnatum with 52.24% inhibition showed the highest percentage of inhibition of F. fujikuroi. In the method of non–volatile metabolites, F. incarnatum and F. proliferatum showed the highest percentage of inhibiting the growth of M. oryzae with 62.27 and 57.96%, respectively. F. incarnatum showed antagonistic activity in three dual culture, volatile metabolites and non–volatile metabolites against M. oryzae and F. fujikuroi fungi. Also, in this experiment, isolates of F. proliferatum and F. incarnatum inhibited the blast disease and crown rot and increased the height and fresh weight of the shoots and roots of rice plants under greenhouse conditions. incarnatum showed antagonistic activity in three dual culture, volatile metabolites and non-volatile metabolites against M. oryzae and F. fujikuroi fungi. Also, in this experiment, isolates of F. proliferatum and F. incarnatum inhibited the blast disease and crown rot and increased the height and fresh weight of the shoots and roots of rice plants under greenhouse conditions. | ||
| کلیدواژهها [English] | ||
| Magnaporthe oryzae, volatile metabolites, inhibition percentage, F. incarnatum, rice endophyte | ||
| مراجع | ||
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Akrami, M. & Yousefi, Z. 2015. Biological control of Fusarium wilts of tomato (Solanum lycopersicum) by Trichoderma spp. as antagonist fungi. Biological Forum, 7(1): 887–892. Aly, A.H., Debbab, A. & Proksch, P. 2011. Fungal endophytes: unique plant inhabitants with great promises. Applied Microbiology Biotechnology, 90: 1829–1845. Araghi, M.M. & Rahnama, K. 2008. Evaluation of biological control of Fusarium graminearum by two antagonist fungi Trichoderma virens and Trichoderma harzianum in vitro. Pajouhesh–Va–Sazandegi, 81: 197–199. (In Persian with English summary) Atugala, D.M. & Deshappriya, N. 2015. Effect of endophytic fungi on plant growth and blast disease incidence of two traditional rice varieties. Journal of the National Science Foundation of Sri Lanka, 43(2): 173–187. Bacon, C.W., Glenn, A.E. & Yates, IE. 2008. Fusarium verticillioides: managing the endophytic association with maize for reduced fumonisins accumulation. Toxin Rev, 27: 411–446. Barnett, H.L. & Hunter, B.B. 1998. Illustrated genera of imperfect fungi. vol Ed. 4. American Phytopathological Society (APS Press) Dingle, J. & Mcgee, DA. 2003. Some endophytic fungi reduce the density of pustules of Puccinia recondita f. sp. tritici in wheat. Mycological Research, 107: 310–316. Blumenstein, K., Alberectsen, B.R., Martin, J.A., Hultberg, M., Sieber, T.N., Helander, M. & Witzell. J. 2015. Nutritional niche overlap potentiates the use of endophytes in biocontrol of a tree disease. Biocontrol, 60(5): 655–667. Cardoso, J.E., Santos, A.A., Rossetti, A.G. & Vidal, J.C. 2004. Relationship between incidence and severity of cashew gummosis in semiarid north–eastern Brazil. Plant Pathology, 53: 367–363. Coombs, J.T. & Franco, C.M.M. 2003. Isolation and identification of actinobacteria from surface–sterilized wheat roots. Appl Environ Microbiol, 69: 5603–5608. De Hoog, G.S., Guarro, J., Gene, J. & Figueras, M.J. 2000. Atlas of clinical fungi. 2nd edition. Centraalbureau voor Schimmelcultures, Utrecht, 1126 pp. Dennis, C. & Webster, J. 1971. Antagonistic properties of species–groups of Trichoderma. I. Production of nonvolatile antibiotics. Transactions of the British Mycological Society, 57(1): 25–39. Dingle, J. & Mc Gee, D.A. 2003. Some endophytic fungi reduce the density of pustules of Puccinia recondita f.sp. tritici in wheat. Mycological Research, 107: 310–316. Ellis, M.B. 1971. Dematiaceous Hyphomycetes. CMI, Kew, England, 608 pp Estrada, A.E.R., Jonkers, W., Kistler, H.C. & May, G. 2012. Interactions between Fusarium verticillioides, Ustilago maydis, and Zea mays: an endophyte, a pathogen, and their shared plant host. Fungal Genet Bio, l49: 578–587. Ganjali, R., Sharif Nabi, B. & Mirlouhi, A.F. 2004. Classical methods and specific primers in detection of endophytic fungi in some gramineuos plants. Rostaniha, 5(1): 37–51. (In Persian with English summary) Ganley, R.J., Brunsfeld, S.J. & Newcombe, G. 2004. A community of unknown endophytic fungi in western white pine. Proceedings of the National Academy of Sciences of the United States of America, 101: 10107–10112. Gautam, A.K. & Avasthi, S. 2019. Fungal endophytes: potential biocontrol agents in agriculture, In: Kumar A, et al. (Eds.), Role of plant growth promoting microorganisms in sustainable agriculture and nanotechnology. Elsevier Inc, 240–298. Gao, F., Dai, C. & Liu, X. 2010. Mechanisms of fungal endophytes in plant protection against pathogens. African Journal of Microbiology Research, 4(13): 1346–1351. Hatamzadeh, S., Rahnama, K., White, J.F., Akbari Oghaz, N., Nasrollahnejad, S. & Hemati, KH. 2022. Investigation of some endophytic fungi from five medicinal plants with growth promoting ability on maize (Zea mays L.). Journal of Applied Microbiology, 134(1): 1–15. Hardoim, P.R., Van Overbeek, L.S., Berg, G., Pirttila, A.M, Compant, S., Campisano, A., Doring, M. & Sessitsch, A. 2015. The hidden world within plants: ecological and evolutionary considerations for defining functioning of microbial endophytes. Microbiol Mol Biol Rev, 79: 293–320. Horsfall, J.G. & Barratt, R.W. 1945. An improved grading system for measuring plant diseases. Phytopathology, 35: 655. IRRI. 2002. Standard evaluation system for rice (SES). 56p. Islam, S., Akanda, A.M., Prova, A., Sultana, F. & Hossain, M.D.M. 2014. Growth promotion effect of Fusarium spp. PPF1 from bermudagrass (Cynodon dactylon) rhizosphere on Indian spinach (Basella alba) seedlings are linked to root colonisation. Arch Phytopathol Plant Prot, 47:2319–31. https://doi.org/10.1080/03235408.2013.876745 Jiménez–Gasco, M.M., Malcolm, G.M., Berbegal, M., Armengol, J. & Jiménez Díaz, R.M. 2014. Complex molecular relationship between vegetative compatibility groups (VCGs) in Verticillium dahliae: VCGs do not always align with clonal lineages. Phytopathology, 104: 650–659. Khodaei, A., Arzanlou, M. & Babai Ahari, A. 2012. Inhibitory effects of three Trichoderma species against three species of Fusarium in laboratory conditions. Journal of Agricultural Science and Sustainable production, 22(4.1): 105–115. (In Persian with English summary). Köberl, M., Schmidt, R., Ramadan, E.M, Bauer. R. & Berg, G. 2013. The microbiome of medicinal plants: diversity and importance for plant growth, quality and health. Front Microbiol, 4: 400. Larran, S., Perelló, A., Simón, M.R. & Moreno, V. 2002. Isolation and analysis of endophytic microorganisms in wheat (Triticum aestivum L.) leaves. World Journal of Microbiology and Biotechnology, 18: 683–686. Liang, H., Xing, Y., Chen, J., Zhang, D., Guo, S. & Wang, C. 2012. Antimicrobial activities of endophytic fungi isolated from Ophiopogon japonicus (Liliaceae). BMC Complement and Alternative Medicine, 12: 238–243. Macia–Vicente, J.G., Jansson, H.B., Mendgen, K. & Lopez–Llorca, L.V. 2008. Colonization of barley roots by endophytic fungi and their reduction of take–all caused by Gaeumannomyces graminis var. tritici Canadian Journal of Microbiology, 54: 600–609. Mandal, S.D., Sonali Singh, S., Hussain, K. & Hussain, H. 2021. Plant–microbe association for mutual benefits for plant growth and soil health. Current Trends in Microbial Biotechnology for Sustainable Agriculture. Springer, 95–121. Martinez–Arias, C., Macaya–Sanz, D. & Witzell, J. 2018. Enhancement of Populus alba tolerance to Venturia tremulae upon inoculation with endophytes showing in vitro biocontrol potential. European Journal of Plant Pathology, 153(4): 1031–1042 Mejía, L.C., Rojas, E.I., Maynard, Z., Van Bael, S., Arnold, A.E, Hebbar, P., Samuels, G.J., Robbins, N. & Herre, E.A. 2008. Endophytic fungi as biocontrol agents of Theobroma cacao pathogens. Biolcontrol, 46 (1): 4–14. DOI: 10.1016/j.biocontrol.2008.01.012. Moricca, S., Ginetti, B. & Ragazzi, A. 2012. Species– and organ–specificity in endophytes colonizing healthy and declining Mediterranean oaks. Phytopathologia Mediterranea, 51: 587–598. Mousavi, S.H., Babaee Zad, V., Sharifnabi, B., Tajik Ghanbari, M.A., Massah, A. & Alavi, S.M. 2014. Induction of blast disease resistance in rice plants by endophyte fungus Priformospora indica. Iranian Journal of Plant Pathology, 153(3): 127–129. (In Persian with English summary). Nassimi. Z. & Taheri, P. 2017. Endophytic fungus Piriformospora indica induced systemic resistance against rice sheath blight via affecting hydrogen peroxide and antioxidants. Biocontrol Science and Technology, 27(2): 252–267. Nel, B., Steinberg, C., Labuschagne, N. & Viljoen, A. 2006. The potential of nonpathogenic Fusarium oxysporum and other biological control organisms for suppressing Fusarium wilt of banana. Plant Pathology, 55: 217–223. Nelson, P.E., Toussoun, T.A. & Marasas, W.F.O. 1983. Fusarium species: an illustrated manual for identification. The Pennsylvania State University Press, University Park. Pili, N.N., Franka, S.C., Kynd, T., Makumba, B.A., Skilton, R., Mibey, M., Mibey, R.K. & Gheysen. G. 2016. Analysis of fungal endophytes associated with rice roots from irrigated and upland ecosystem in Kenya. Plant and Soil, 405(1–2): 371–380. Prasetyawan, Y, wati, S.R., wati, I.R., suwarso, W.P. & sumaryono, W. 2012. Isolation and Identification of Endophytic Fungi from Srikaya Plants (Annona squamosa) having potential secondary metabolites as anti–breast aancer activity. Microbiology Indonesia, 6(1): 23. Putri, R.E.S., Djauhari, S. & Martosudiro, M. 2021. Endophytic Fungi as Potential Agents of Helminthosporium sp. in Rice Plant (Oryza sativa L.), 2(2): 50–60. https://doi.org/10.21776 ub. jtpp. 2021. 002.2.3. Putri, N.D., Muhibuddin, A. & Luqman, L.Q.A. 2021. The Potential of Endophytic Fungi in Promoting Rice Plant Growth and Suppressing Blast Disease, 2(2): 41–49. https://doi.org/10.21776 ub.jtpp.2021.002.2.2. Qadri, M., Johri, S., Shah, B.A., Khajuria, A., Sidiq, T., Lattoo, S.K., Abdin, M.Z. & Riyaz –Ul–Hassan, S. 2013. Identification and bioactive potential of endophytic fungi isolated from selected plants of the Western Himalayas. SpringerPlus, 2: 8–22. Ramesh, N.K. Naeimi, S., Rezaee, S. & Fotouhifar, K.B. 2020. Biological control of rice Bakanae disease caused by Fusarium fujikuroi using some endophytic fungi. Entomology and Phytopathology, 87(2): 281–296. (In Persian with English summary). Razavi, S. E., Sanei, S. J. Sharbatkhari, M. & Ghorbani Nasr Abadi, R. 2022. Detection and isolation of soil fungi and pseudo–fungi. Peyk reyhan, 384 pp. Rigobelo, E.C. & Baron, N.C. 2021. Endophytic fungi a tool for plant growth promotion and sustainable agriculture. Mycology, 1–17. Sieber, T.N. & Grunig, C.R. 2013. Fungal root endophytes. In: Eshel A., Beeckman T. (eds): Plant Roots –The Hidden Half, CRC Press, Taylor & Francis Group, Boca Raton, FL, USA, 38(1): 38–49. Sivakumar, D., Wilson Wijeratnam, R.S., Wijesundera, R.L.C., Marikar, F.M.T. & Abeyesekere, M. 2000. Antagonistic effect of Trichoderma harzianum on postharvest pathogens of Rambutan (Nephelium lappaceum). Phytoparasitica, 28(3): 240–247. Sivanesan, A. 1987. Gramainicolous species of Bipolaris, Curvularia, Drechslera, Exserohilum and their teleomorphs. CAB International Mycological Institute, 261 pp. Solla, A. & Gil, L. 2012. Evaluating Verticillium dahliae for biological control of Ophiostoma novo–ulmiin Ulmus minor. Plant Pathol, 52: 579–585. http://dx.doi.org/10. 1046/j.1365–3059.2003. 00921.x Su, Z.Z., Mao, L.J., Li, N., Fenf, X.X., Yuan, Z.L., Wang, L.W., Lin, F.C. & Zhang, C.L. 2013. Evidence for Biotrophic Lifestyle and Biocontrol Potential of Dark Septate Endophyte Harpophora oryzae to Rice Blast Disease. Plos one, 8(4): 61332. Suada, I.K., Suhartini, D.M.W.Y., Sunariasih, N.P.L., Wirawan, I.P.W., Chun, K.W., Cha, J.Y. & Ohga, S. 2012. Ability of endophytic fungi isolated from rice to inhibit Pyricularia oryzae induced rice blast in Indonesia, Journal of the Faculty of Agriculture, Kyushu University, 57(1): 51–53. Sun, X., Ding, Q., Hyde, K.D. & Guo, L.D. 2012. Community structure and preference of endophytic fungi of three woody plants in a mixed forest. Fungal Ecology, 5(5): 624–632. Tian, X.L., Cao, L.X., Tan, H.M., Zeng, Q.G., Jia, Y.Y., Han, W.Q. & Zhou, S.N. 2004. Study on the communities of endophytic fungi and endophytic Actinomycetes from rice and their antipathogenic activies in vitro, World Journal of Microbiology & Biotechnology, 20(3): 303–309. Wani, Z.A., Ashraf, N., Mohiuddin, T. & Riyaz–ul–Hassan, S. 2015. Plant–endophyte symbiosis, an ecological perspective. Applied Microbiology and Biotechnology, 99(7): 2955–2965. Youssef, S.A., Tartoura, K.A. & Abdelraouf, G.A. 2016. Evaluation of Trichoderma harzianum and Serratia proteamaculans effect on disease suppression, stimulation of ROS scavenging enzymes and improving tomato growth infected by Rhizoctonia solani. Biological Control, 100: 79–86. Yuan, Z.L., SU, Z.Z., Mao, L.J., Peng, Y.Q., Yang, G.M., Lin, F.C. & Zhang, C.L. 2011. Distinctive endophytic fungal assemblage in stems of wild rice (Oryza granulata) in China with special reference to two species of Muscodor (Xylarriaceae). The Journal of Microbiology, 49(1): 15–23. Zakaria, L., Yaakop, A.S., Salleh, B. & Zakaria, M. 2010. Endophytic fungi from paddy. Tropical Life Science Research, 21(1): 101–107. | ||
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