Effects of some essential oils against growth and biofilm production of two plant pathogenic bacteria, Pectobaterium carotovorum and Pectobacterium atrosepticum | ||
| مهار زیستی در گیاهپزشکی | ||
| Volume 9, Issue 2 - Serial Number 18, March 2022, Pages 135-151 PDF (769.01 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/bcpp.2023.360551.324 | ||
| Authors | ||
| Milad Aeini* 1; Hossein Mirzaei Najafgholi2; Fatemeh Derikvand3; Mitra Khademi4; Neda Rouhani5 | ||
| 1Plant protection department, Faculty of agriculrure, Shahid Chamran university of Ahvaz | ||
| 2Assistant Professor, Plant Protection Department, Faculty of Agriculture, Lorestan university, Khorram Abad, Iran | ||
| 3Plant Protection Department, Faculty of Agriculture, Lorestan university, Khorram Abad, Iran | ||
| 4Production engineering and plant genetics department, Faculty of Agriculture, Lorestan university, Khorram Abad, Iran | ||
| 5Plant Protection department, Faculty of Agriculture, Lorestan university, Khorram Abad, Iran | ||
| Abstract | ||
| Plant–derived essential oils (EOs) are a major group of environments–friendly compounds with antibacterial properties that have been used during the last decade. This study aimed to identify the chemical composition and evaluate the antimicrobial activity of EOs from Lippia citriodora, Origanum vulgare, Phlomis fruticose, Satureja spicigera, and Syzygium aromaticum against two soft rot– including bacteria, Pectobacterium carotovorum and Pectobacterium atrosepticum. Plants were collected from Khuzestan province, and their essential oil composition was determined from gas–chromatography–mass spectrometry (GC–MS) analysis. The antibacterial effect of Eos was measured by the disk diffusion method. The effect of EOs on biofilm formation was also assayed. In addition, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were calculated accordingly. The interaction among the EOs was calculated according to the fractional inhibitory concentration (FIC) index using the Modified Dilution Checkboard method. The result showed that all Eos inhibited bacterial growth. The highest MIC (31/25) was attributed to P. fruticosa against P. carotovorum while the lowest MIC (125) belongs to L. citriodora and S. spicigera against P. carotovorum. FIC index showed that L. citriodora and S. spicigera are neutral to P. carotovorum. Analysis of GC–MS chromatogram indicated that the major constituents of P. fruticosa, L. citriodora, O. vulgare, S. spicigera, and S. aromaticum were cis–Thujone (17.47%), Isogeraniol (23%), cis Piperitone oxide (29.5%), Carvacrol (88.5%), Eugenol (98.7%), respectively. According to the results, in high MIC of Eos (125 ppm), bacteria were not able to form biofilm. Our results indicate that these five plant species have antimicrobial activity against Pectinbacterium species. Among all Eos used, L. citriodora and P. fruticose showed the highest activity which, therefore, can be recommended for further applications. | ||
| Keywords | ||
| Disk diffusion; medicinal plant; inhibition; pathogenesis | ||
| References | ||
|
Ahmadi, A., Mirzaei Najafgholi, H., Aeini, M. & Kakolvand, K. 2021. Isolation and Identification of Othello, Atlantis, and Puma Super Herbicide–resistant Bacteria Isolated from the Soil of Wheat Farms. Biological Journal of Microorganism, 10(37): 67–77.
Baghaee–Ravari, S., Moslemkhani, K. & Khodaygan, P. 2013. Assessment of genetic variability of prevalent pectinolytic bacteria causing potato tuber soft rot in eastern Iran. Journal of Plant Pathology, 95: 107–113.
Baghaee–Ravari, S., Rahimian, H., Shams–Bakhsh, M., Lopez–Solanilla, E., Antúnez–Lamas, M. & Rodríguez–Palenzuela, P. 2011. Characterization of Pectobacterium species from Iran using biochemical and molecular methods. European Journal of Plant Pathology, 129(3): 413–425.
Bhardwaj, S.K. & Laura, J.S. 2008. Antibacterial activity of some plant extracts against pathogenic bacteria Erwinia carotovora subsp. carotovora. Potato Journal, 35:1–2.
Borges, D.F., Lopes, E.A., Moraes, A.R.F., Soares, M.S., Visôtto, L.E., Oliveira, C.R. & Valente, V.M.M. 2018. Formulation of botanicals for the control of plant–pathogens: A review. Crop Protection, 110: 135–140.
Cariddi, C. & Sanzani, S.M. 2013. A severe outbreak of bacterial lettuce soft rot caused by Pectobacterium carotovorum subsp. carotovorum in Apulia (Italy). Journal of Plant Pathology, 95: 441–446.
Carson, C.F., Hammer, K.A. & Riley, T.V. 2006. Melaleucaa lternifolia (Tea Tree) oil: Review antimicrobialnd other medicinal properties. Journal of Microbiology, 19: 50–62.
Charkowski, A.O. 2015. Biology and control of Pectobacterium in potato. American Journal of Potato Resarch, 92(2): 223–9.
Davidsson, P.R., Kariola, T., Niemi, O. & Palva, T. 2013. Pathogenicity of and plant immunity to soft rot pectobacteria. Frontiers in Plant Science, 4: 191.
EL–Hefny, M., Ashmawy, N.A., Salem, M.Z.M. & Salem, A.Z.M. 2017. Antibacterial activities of the phytochemicals–characterized extracts of Callistemon viminalis, Eucalyptus camaldulensis and Conyza dioscoridis against the growth of some phytopathogenic bacteria. Microbial Pathogenesis, 113: 348–356.
Emiroglu, Z. K., Yemis, G.P., Coskun, B.K. & Candogan, K. 2010. Antimicrobial activity of soy edible films incorporated with thyme and oregano essential oils on fresh ground beef patties. Meat Science, 86(2): 283–288.
Pillai, S.K., Moellering, R.C. & Eliopoulos, G.M. 2005. Antimicrobial Combinations. In: Lorian, V., Ed., Antibiotics in Laboratory Medicine, 5th Edition, the Lippincott Williams & Wilkins Co., Philadelphia, 365–440.
Gebarowska, E., Politowicz, J. & Szumny, A. 2017. Chemical composition and antimicrobial activity of Geranium robertianum L. Essential oil. Acta Poloniae Pharmaceutica, 74(2): 699–705.
Gerayeli, N., Baghaee–Ravari, S. & Tarighi, S. 2018. Evaluation of the antagonistic potential of Bacillus strains against Pectobacterium carotovorum subsp. carotovorum and their role in the induction of resistance to potato soft rot infection. European Journal of Plant Pathology, 150(4): 1049–1963.
Ghalem, B.R. 2016. Essential oils as antimicrobial agents against some important plant pathogenic bacteria and fungi. In: Choudhary, D.K., Varma, A., Tuteja, N. (Eds.), Eds.) Plant–Microbe Interaction: An Approach to Sustainable Agriculture. Springer, Singapore, 271–296.
Gursoy, U.K., Gursoy, M., Gursoy, O.V., Cakmakci, L., Könönen, E. & Uitto, V.J. 2009. Anti–biofilm properties of Satureja hortensis L. Essential oil against periodontal pathogens. Anaerobe, 15(4): 164–167.
Gutierrez–Pacheco, M.M., Gonzalez–Aguilar, G.A., Martinez–Tellez, M.A., LizardiMendoza, J., Madera–Santana, T.J. & Bernal–Mercado, A.T. 2018. Carvacrol inhibits biofilm formation and production of extracellular polymeric substances of Pectobacterium carotovorum subsp. Carotovorum. Food Control, 89: 210–218.
Hall–Stoodley, L., Costerton, J.W. & Stoodley, P. 2004. Bacterial biofilms: from the natural environment to infectious diseases. Nature Reviews Microbiology, 2(2): 95.
Darvishnia, M., Mirzaei–najafgholi, H., Aeini, M. & Joshaghani, A. 2021. Identification and genotypic characteristics of Erwinia amylovora isolates, the causal agents of fire blight on pome fruit trees in Hamadan province. Plant Protection (Scientific Journal of Agriculture), 43(4): 19–34.
Huang, Q. & Lakshman, D.K. 2010. Effect of clove oil on plant pathogenic bacteria and bacterial wilt of tomato and geranium. Journal of Plant Pathology, 92: 701–707.
Hyldgaard, M., Mygind, T. & Meyer, R.L. 2012. Essential oils in food preservation: mode of action, synergies, and interactions with food matrix components. Frontiers in Microbiology, 3: 12.
Ikeura, H. & Kobayashi, F. 2015. Antimicrobial and antifungal activity of volatile extracts of 10 herb species against Glomerella cingulata. Journal of Agricultural Science, 7(9): 77.
Jeong, M.R., Park, P.B., Kim, D.H., Jang, Y.S., Jeong, H.S. & Choi, S.H. 2009. Essential oil prepared from Cymbopogon citrates exerted an antimicrobial activity against plant pathogenic and medical microorganisms. Mycobiology, 37(1): 48–52.
Kalu, C.M., Kanu, S.A., Gangireddygari, V.S.R. & Ntushelo, K. 2018. Effect of Solanum mauritianum leaf extract on the growth and morphology of Pectobacterium carotovorum subsp. carotovorum, P. carotovorum subsp. brasiliense and Bacillus subtilis. South African Journal of Botany, 115: 289.
Kavanaugh, N.L. & Ribbeck, K. 2012. Selected antimicrobial essential oils eradicate Pseudomonas spp. And Staphylococcus aureus biofilms. Applied and Environmental Microbiology, 778: 4057–61.
Kerekes, E.B., Deák, É., Takó, M., Tserennadmid, R., Petkovits, T., Vágvölgyi, C. & Krisch, J. 2013. Anti‐biofilm forming and anti‐quorum sensing activity of selected essential oils and their main components on food‐related micro‐organisms. Journal of Applied Microbiology, 115(4): 933–942.
Kim, Y.G., Lee, J.H., Gwon, G., Kim, S.I., Park, J.G. & Lee, J. 2016. Essential oils and eugenols inhibit biofilm formation and the virulence of Escherichia coli O157: H7. Scientific Reports, 6: 36377.
Mantsebo, C.C., Mazarura, U., Goss, M. & Ngadze, E. 2014. The epidemiology of Pectobacterium and Dickeya species and the role of calcium in postharvest soft rot infection of potato (Solanum tuberosum) caused by the pathogens: a review. African Journal of Agricultural Research, 9(19):1509–15.
Mari, M., Bautista–Baños, S. & Sivakumar, D. 2016. Decay control in the postharvest system: role of microbial and plant volatile organic compounds. Postharvest Biology and Technology, 122: 70–81.
Mehrsorosh, H., Gavanji, S., Larki, B., Mohammadi, M.D., Karbasiun, A. & Bakhtari, A. 2014. Essential oil composition and antimicrobial screening of some Iranian herbal plants on Pectobacterium carotovorum. Global Nest Journal, 16: 240–250.
Moghaddam, M. & Mehdizadeh, L. 2017. Chemistry of Essential Oils and Factors Influencing Their Constituents. In: Soft Chemistry and Food Fermentation, 379–419.
Mirzaei–Najafgholi, H., Tarighi, S., Golmohammadi, M. & Taheri, P. 2017. The effect of citrus essential oils and their constituents on growth of Xanthomonas citri subsp. citri. Molecules, 14: 22(4):591.
Mirzaei–Najafgholi, H., Aeini, M., Tarighi, S. & Golmohammadi, M. 2021. Comparing bacterial properties in relation to the virulence factors of Xanthomonas citri subsp. citri strains and evaluating resistance of subtribe Citrinae cultivars to the most virulent strain. Journal of Plant Pathology, 103: 449–460.
Mirzaei, H., Khademi, M., Nazarian_Firouzabadi, F. & Derikvand, F. 2022. Evaluation of the synergistic effect of dermasptin B1 recombinant proteins and essential oils against gram–negative phytopathogens using fractional Inhibitory Concentration Index (FIC). Biological Journal of Microorganism, (Accepted paper).
Nissen, L., Zatta, A., Stefanini, I., Grandi, S., Sgorbati, B., Biavati, B. & Monti, A. 2010. Characterization and antimicrobial activity of essential oils of industrial hemp varieties (Cannabis sativa L.). Fitoterapia, 81(5): 413–419.
O’Toole, G.A. 2011. Microtiter dish biofilm formation assay. Journal of Visualized experiments: 47.
Omidi Nasab, M. & Aeini, M. 2020. Composition and antibacterial activity of the chemical essential oil of Foeniculum vulgare and Eucalyptus to control some important plant pathogenic bacteria. Journal of Microbial World, 12(4): 393–399.
Olsen, N., Miller, J. & Nolte, P. 2006. Diagnosis & Management of Potato Storage Diseases. University of Idaho extension publication CIS 1131.
Packiavathy, I.A.S.V., Priya, S., Pandian, S.K. & Ravi, A.V. 2014. Inhibition of biofilm development of uropathogens by curcumin–an anti–quorum sensing agent from Curcuma longa. Food Chemistry, 148: 453–460.
Paradza, V.M., Icishahayo, D. & Ngadze, E. 2012. Efficacy of botanical extracts from garlic and neem on controlling potato soft rot pathogens. UNISWA J Agric 16, 1–10. Pinhero, G.P., Yada, R.Y., 2016. Postharvest storage of potatoes. In: Singh, J., Kaur, L. (Eds.), Advances in Potato Chemistry and Technology. Academic Press, London, 283–314.
Pinhero, G.P. & Yada, R.Y. 2016. Postharvest storage of potatoes. In: Singh, J., Kaur, L. (Eds.), Advances in Potato Chemistry and Technology. Academic Press, London, pp. 283–314.
Ramezanian, A., Azadi, M., Mostowfizadeh–Ghalamfarsa, R. & Saharkhiz, M.J. 2016. Effect of Zataria multiflora Boiss and Thymus vulgaris L. Essential oils on black rot of ‘Washington Navel’ orange fruit. Postharvest Biology and Technology, 112: 152–158.
Szczepanski, S. & Lipski, A. 2014. Essential oils show specific inhibiting effects on bacterial biofilm formation. Food Control, 36(1): 224–229.
Zaidi–Yahiaoui, R., Zaidi, F. & Bechar, S. 2008. Effect of Olea europaea L and Salvia officinalis leaves phenolics extracts on potato tubers soft rot disease. Planta Medica, 74 (09):PE9.
Zaker, S., Gavanji, S., Sayedipour, S., Bakhtari, A., Shirani Bidabadi, E., Larki, B. & Golestannejad, Z. 2014. The effect of some herbal essential oils on pathogenic bacteria. Ethno–Pharmaceutical Products, 1(2): 23–34.
Zivanovic, S., Chi, S. & Draughon, A.F. 2006. Antimicrobial activity of chitosan films enriched with essential oils. Journal of Food Science, 70: 45–51. | ||
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