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Abbaszadeh, F., Daneshvar, M., Salehi Salmi, M., Lotfi Jalal Abadi, A. (2023). Determining the best combination of explant and plant growth regulators on callogenesis, indirect organogenesis, proliferation, and rooting of Lavandula officinalis L.. , 30(2), 306-323. doi: 10.22092/ijrfpbgr.2022.359072.1423
F. Abbaszadeh; M. H. Daneshvar; M. Salehi Salmi; A. Lotfi Jalal Abadi. "Determining the best combination of explant and plant growth regulators on callogenesis, indirect organogenesis, proliferation, and rooting of Lavandula officinalis L.". , 30, 2, 2023, 306-323. doi: 10.22092/ijrfpbgr.2022.359072.1423
Abbaszadeh, F., Daneshvar, M., Salehi Salmi, M., Lotfi Jalal Abadi, A. (2023). 'Determining the best combination of explant and plant growth regulators on callogenesis, indirect organogenesis, proliferation, and rooting of Lavandula officinalis L.', , 30(2), pp. 306-323. doi: 10.22092/ijrfpbgr.2022.359072.1423
Abbaszadeh, F., Daneshvar, M., Salehi Salmi, M., Lotfi Jalal Abadi, A. Determining the best combination of explant and plant growth regulators on callogenesis, indirect organogenesis, proliferation, and rooting of Lavandula officinalis L.. , 2023; 30(2): 306-323. doi: 10.22092/ijrfpbgr.2022.359072.1423

Determining the best combination of explant and plant growth regulators on callogenesis, indirect organogenesis, proliferation, and rooting of Lavandula officinalis L.

تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران
Article 11, Volume 30, Issue 2 - Serial Number 60, March 2023, Pages 306-323    PDF (1.04 M)
Document Type: Research Paper
DOI: 10.22092/ijrfpbgr.2022.359072.1423
Authors
F. Abbaszadeh1; M. H. Daneshvar2; M. Salehi Salmi* 3; A. Lotfi Jalal Abadi4
1M.Sc. Graduated, Dept. Horticultural Science, Agricultural Sciences and Natural Resources University of Khuzestan, Iran
2Prof. Dept. Horticultural Science, Agricultural Sciences and Natural Resources University of Khuzestan, Iran.
3Assoc. Prof. Dept. Horticultural Science, Agricultural Sciences and Natural Resources University of Khuzestan, I.R. Iran.
4Assist. Prof. Dept. Plant Production and Genetics, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, I.R. Iran.
Abstract
Lavender (Lavandula officinalis L.) is one of the rangeland species in the many parts of the world, which is cultivated as a medicinal and ornamental plant. Plant tissue culture techniques are an alternative method for the mass production of lavender colons and can overcome the problems caused by reproductive propagation. To determine the effect of different combinations and concentrations of growth regulators, and also explant type in the process of callogenesis, organogenesis, and rooting of lavender, a factorial experiment was conducted based on a completely randomized design with three replications. In this research, different responses to growth regulatory compounds were assessed at different stages. The best treatment for callogenesis was cotyledon explants in culture medium containing 0.5 mg/l 2.4-D along with 0.25 mg/l BAP. In indirect organogenesis, the highest number of branches and the rate of organogenesis were obtained in the medium containing 2 mg/l of KIN. Also, the highest number of branches was obtained from using 0.5 mg/l BAP treatment with 0.5 mg/l of KIN along with 0.1 mg/l IBA. The results showed that the best culture medium for rooting was the medium containing 1 mg/l IBA. The highest explants survival rate was related to using perlite and coco peat as a bed of cultivation. The finding of this research introduced an efficient regeneration system for this plant that can be used in rapid vegetative propagation, germplasm maintenance, and molecular modification programs through genetic engineering.
 
Keywords
Subshrub; in vitro; micropropagation; cytokinin; heterogeneity
References
  • Abbasi, B., Saxena, P. K., Murch, S. J. and Liu, C. Z., 2007. Echinacea biotechnology: Challenges and opportunities. In Vitro Cellular and Development Biology- Plant, 43: 481-492.
  • Afzalifar, M., 2012. Evaluation of different propagation and androgenesis in Satureja khuzistanica and rechingeri. M.Sc. thesis. Medicinal Plants and Drug Research Institute. Shahid Beheshti University, Iran. 46-73. (In Persian).
  • Asghari, F., Hasani, A., Hosseini, B. and Farokhi, J., 2013. Effect of different hormonal combination on in vitro direct shoot regeneration of basil (Ocimum basilicum). Journal of Horticultural Science 26(4): 434-439. (In Persian).
  • Bazrafkan, M., Daneshvar, M. H. and Salehi Salmi, M. R., 2019. Effects of explant type, culture media and growth regulators on callus induction and plant regeneration of sweet pea (Lathyrus odoratus). Iranian Journal of Rangelands and Forests Pant Breeding and Genetics Research, 27: 98-107. (In Persian).
  • Chan, L. K., Koay, S. S., Low, P. H. and Boey, P. L., 2008. Effect of plant growth regulators and subculture frequency on callus culture and the establishment of Melastomamala bathricum cell suspension cultures for the production of pigments. Biotechnology, 7 (4): 678-685.
  • Chawla, H. S., 2009. Introduction to plant biotechnology. 3rd ed. Enfield: Science Publishers 710p.
  • Dias, M. C., Almeida, R. and Romano, A., 2002. Rapid clonal multiplication of Lavandula viridis L'Her through in vitro axillary shoot proliferation. Plant Cell, Tissue and Organ Culture, 68: 99-102.
  • Dronne, S., Colson, M., Moja, S. and Faure, O., 1999. Plant regeneration and transient GUS expression in a range of lavender (Lavandula intermedia Emeric ex Loiseleur) cultivars. Plant Cell Tissue and Organ Culture, 55: 193-198.
  • George, E. F., Hall, M. A. and. Klerk, G. J. D., 2008. Plant propagation by tissue culture, Volume 1. The Background. Springer, 502 p.
  • Hadipour, A., Hoseini Mazinani, M. and Mehrafarin, A., 2013. Changes in essential oil content/composition and shoot aerial yield of lavender (Lavandula officinalis) affected by different treatments of nitrogen. Journal of Medicinal Plants, 12: 156-169. (In Persian).
  • Panizza, M. and Tognoni, F., 1988. Clonal propagation, callus formation and plant regeneration of lavender. Scientia horticulturae, 37: 157-163.
  • Han, Y., Jin, X., Wu, F. and Zhang, G., 2011. Genotypic differences in callus induction and plant regeneration from mature embryos of barley (Hordeum vulgare), Journal of Zhejiang University Science, 12 (5), PP: 399-407.
  • Hassandokht, M. R. and Ebrahimi, R., 2006. Principles of plant tissue culture. Marz Danesh, 328 p.
  • Huy, N. and Xuan-Mai, T., 2014. Investigation of effective in vitro propagation media for Stevia rebaudiana Asia-Pacific Journal Science Technology, 19: 172-180.
  • Jafari, S., Daneshvar, M. H., Salehi Salmi, M. R. and Lotfi, A., 2017. Influence of putrescine and thidiazuron on in vitro organogenesis in Salvia officinalis Iranian Journal of Rangelands and Forests Pant Breeding and Genetics Research, 25: 201-211. (In Persian).
  • Jordan, A. M., Calvo, M. C. and Segura, J., 1998. Micropropagation of adult Lavandula dentata Journal of Horticulture Science and Biotechnology, 73:93-96.
  • Kafi, M., Zand, A., Kamkar, B., Sharifi, H. and Goldani, M., 2006. Plant Physiology (Taiz and Zeiger). University Jihad Publications. Sixth edition. Vol 2. 379 Pp. (In Persian).
  • Kazem, S., 2012. Optimization of tissue culture and gene transformation in (Arctium lappa), Biotechnology, Master of Science Thesis, Dept., of Agronomy and Plant Breeding, Razi University, Kermanshah. Iran. (In Persian).
  • Kharrazi, M., Sharifi, A., Keykha Akhar, F., Bagheri, A. and Moradian, M., 2018. Effect of hormonal compositions on micropropagation of fifteen cultivars of gerbera (Gerbera Jamesonii bolus ex hooker f.). Plant Productions, 40: 91-102. (In Persian).
  • Khorasaninejad, S., Soltanloo, H., Ramezanpour, S.S., Hadian, J. and Atashi, S., 2016. The effect of drought stress on the growth, essential oil yield and chemical composition of Lavender. Journal of Crops Improvement, 17: 979-988. (In Persian).
  • Mohamadi, Z., 2010. Study of callus, regeneration and suspension cultures of different genotypes of chamomile (Matricaria chamomilla). Master's Thesis in Plant Breeding, Agronomy and Plant Breeding Dept, Faculty of Agriculture, Ilam University. (In Persian).
  • Mok, M. C., Martin, R. C. and Mok, D. W. C., 2000. Cytokinins: biosynthesis, metabolism and perception. In Vitro Cellular & Developmental Biology, 36: 102-107.
  • Motamedi, J., Zebarjadi, A. R., Kahrizi, D., Salmanian, A. H. and Soheilikhah, Z. H., 2010. Study of callus induction and shoot regeneration of Safflower (Carthamus tinctorius,) using hypocotyl and cotyledon explants culture, Journal of Agricultural Biotechnology, 1: 99-111.
  • Movafeghi, A., Habibi, G. H. and Aliasgarpoor, M., 2008. Plant regeneration of Capparis spinosa using hypocotyl explant, Iranian Journal of Plant Biology, 21 (2): 289-297. (In Persian).
  • Murashige, T. and Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum, 15: 473-497.
  • Narimani, R., moghadam, M. and Mojarab, S., 2017. Evaluation of the micropropagation of hairless catmint (Nepeta nuda), an endangered medicinal plant. Journal of Cell & Tissue, 7(4): 387-397.
  • Parsamanesh, Z., Bayat, F. and Hedaya, M., 2018. Optimizing in vitro regeneration of Hypericum (Hypericum erforatum). Plant Productions, 40: 103-115. (In Persian).
  • Pandey, A. and Sushma, T., 2012. Influence of kinetin on in vitro rooting and survival of banj oak (Quercus leucotrichophora). African Journal of Biotechnology, 62: 12538-12545.
  • Reichling, J. and Beiderbeck, R., 2009. Chamomilla recutita, Rauschert (Camomile): In vitro culture and the production of secondary metabolites, Medicinal and Aromatic Plants III. Springer, Berlin Heidelberg New York, Pp: 156-175.
  • Sayadi, V., Mehrabi, A. A., Saidi, M. and Nourollahi, K. H., 2014. In vitro culture and callus induction of chamomile (Matricaria chamomilla) explants under different concentrations of plant growth regulators, International Journal of Biosciences, 4 (10): 206-211.
  • Shinoyama, H., Nomura, Y., Tsuchiya, T. and Kazuma, T., 2004. A simple and efficient method for somatic embryogenesis and plant regeneration from leaves of Chrysanthemum (dendranthema ×grandiflorum (Ramat.) Kitamura), Journal Plant Biotechnology, 21 (1): 25-33.
  • Shobi, T. M. and Viswanathan, M. B. G., 2017. Micropropagation of an important medicinal plant, Begonia fallax (Begoniaceae). International Journal of Current Research in Biosciences and Plant Biology, 12: 94-99.
  • Shriram, V., Kumar, V. and Shitole, M.G., 2008. Indirect organogenesis and plant regeneration in Helicteres isora, an important medicinal plant. In vitro Cellular and Developmental Biology-Plant, 44: 186-193.
  • Taghizade, M. and Solgi, M., 2015. Introduction of commercial protocol for in vitro proliferation of Brassica oleracea acephala. Iranian Journal of Horticaltural Sciences 45(4): 475-484. (In Persian).
  • Takano, T., 1992. Germination characteristics of herbs in Labiatae. In WOCMAP I-Medicinal and Aromatic Plants Conference, 275-286.
  • Tonutti, I. and Liddle, P., 2010. Aromatic plants in alcoholic beverages. Flavour and Fragrance Journal, 25: 341-350.
  • Wang, X., Jin, L., Li, M., Zhao, M. and Zhao, H., 2007. Bioreactor culture and plant regeneration from cell clusters of the aromatic plant, Lavandula angustifolia ‘Munstead’. Journal Horticultural Science Biotechnology, 82: 781-785.
  • Yan, M. M., Xu, C., Kim, C. H. and Um, Y. C., 2009. Effects of explant type, culture media and growth regulators on callus induction and plant regeneration of Chines jiaotou (Allium chinense), Scientia Horticulturae, 123: 124-128.
  • Zebarjadi, A. R., Motamedi, M. J., Taravat, E. and Ismaili, A., 2013. Micropropagation of medicinal purple coneflower (Echinacea purpurea) using cotyledon and hypocotyl segments, Journal of Plant Researches (Iranian Journal of Biology), 3(26): 311-319.
  • Zuzarte, M. R., Dinis, A. M., Cavaleiro, C., Salgueiro L. R. and Canhoto, J. M., 2010. Trichomes essential oils and in vitro propagation of Lavandula pedunculata (Lamiaceae). Industrial Crops and products, 32: 580-587.
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