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Alimolaei, Mojtaba, Shamsaddini Bafti, M. (1402). Isolation and Molecular Characterization of Clostridium perfringens Toxinotypes F & G in Diarrhoeic Sheep (Ovis aries) Flocks in Southeast of Iran. سامانه مدیریت نشریات علمی, 78(3), 1159-1168. doi: 10.22092/ari.2023.360450.2582
Mojtaba Alimolaei; M Shamsaddini Bafti. "Isolation and Molecular Characterization of Clostridium perfringens Toxinotypes F & G in Diarrhoeic Sheep (Ovis aries) Flocks in Southeast of Iran". سامانه مدیریت نشریات علمی, 78, 3, 1402, 1159-1168. doi: 10.22092/ari.2023.360450.2582
Alimolaei, Mojtaba, Shamsaddini Bafti, M. (1402). 'Isolation and Molecular Characterization of Clostridium perfringens Toxinotypes F & G in Diarrhoeic Sheep (Ovis aries) Flocks in Southeast of Iran', سامانه مدیریت نشریات علمی, 78(3), pp. 1159-1168. doi: 10.22092/ari.2023.360450.2582
Alimolaei, Mojtaba, Shamsaddini Bafti, M. Isolation and Molecular Characterization of Clostridium perfringens Toxinotypes F & G in Diarrhoeic Sheep (Ovis aries) Flocks in Southeast of Iran. سامانه مدیریت نشریات علمی, 1402; 78(3): 1159-1168. doi: 10.22092/ari.2023.360450.2582

Isolation and Molecular Characterization of Clostridium perfringens Toxinotypes F & G in Diarrhoeic Sheep (Ovis aries) Flocks in Southeast of Iran

Archives of Razi Institute
مقاله 47، دوره 78، شماره 3، مرداد و شهریور 2023، صفحه 1159-1168    اصل مقاله (674.49 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2023.360450.2582
نویسندگان
Mojtaba Alimolaei* 1؛ M Shamsaddini Bafti2
1Department of Molecular Microbiology, Kerman branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Kerman, Iran
2Department of Anaerobic Bacterial Vaccine Research and Production, Kerman Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Kerman, Iran
چکیده
Clostridial enteric diseases, called enterotoxemia, are caused by Clostridium perfringens toxinotypes in sheep and other ruminants. This study aimed to describe the molecular characterization of C. perfringens isolates in diarrhoeic sheep (Ovis aries) flocks in the southeast of Iran. Fecal/intestinal samples were collected from diarrhoeic (n=116), dead (n= 13), and healthy (n=63) sheep over four years (2016-2020) and subjected to bacteriological and molecular examinations. The C. perfringens isolates were typed by polymerase chain reaction targeting genes, namely 16SrRNA, CPA, CPB, ETX, IAP, CPE, and NetB. The overall prevalence of C. perfringens was 28.6% among the studied sheep, and there was a significant relationship between its isolation rate and diarrhea (P<0.001). The C. perfringens isolation rate also decreased with animal age (P=0.012) and was significantly higher in late winter and spring (P=0.000). The most prevalent toxinotypes were types A (52.4%), D (22.2%), and F (18.5%), in that order. Moreover, C, G, and B types were found in 4.2%, 1.6%, and 1.1% of the isolates, respectively, and no type E was detected. The CPE gene was detected in 32.3% of all isolates, and the diarrhoeic sheep were most likely to yield CPE+ strains of C. perfringens (93.1%). These findings highlight the importance of CPE+ strains of C. perfringens in sheep enteritis and suggest that the high presence of type F needs to be considered in new clostridial vaccines containing this toxinotype. It is noteworthy that the present study reported the isolation of C. perfringens type F, type G, and the CPE+ strains of type B from diarrhoeic sheep for the first time.
کلیدواژه‌ها
Clostridium perfringens؛ Diarrhoea؛ Iran؛ Sheep (Ovis aries)؛ Toxinotype
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مراجع
  1. Rood JI, Adams V, Lacey J, Lyras D, McClane BA, Melville SB, et al. Expansion of the Clostridium perfringens toxin-based typing scheme. Anaerobe. 2018;53:5-10.
  2. Goekce HI, Genç O, Soezmen M, Gökçe G. Determination of Clostridium perfringens toxin-types in sheep with suspected enterotoxemia in Kars Province, Turkey. Turk J Vet Anim Sci. 2007;31(5):355-60.
  3. Hayati M, Tahamtan Y. Toxin typing of Clostridium perfringens Associated with Enterotoxaemia in Sheep in Fars Province. Arch Razi Inst. 2021;76(3):691.
  4. Hosseinzadeh S, Bahadori M, Poormontaseri M, Dehghani M, Fazeli M, Nazifi S. Molecular characterization of Clostridium perfringens isolated from cattle and sheep carcasses and its antibiotic resistance patterns in Shiraz slaughterhouse, southern Iran. Veterinarski Arhiv. 2018;88(5):581-91.
  5. Fahimeh Y, Peyman N, Gholamreza H, Gholamali K, Mohammad R, Jamshid R. Major and minor toxins of Clostridium perfringens isolated from healthy and diseased sheep. Small Rumin Res. 2018;168:1-5.
  6. Jabbari A, Afshari FS, Esmaelizad M, Pilehchian LR, Moosawi SM, Abdolmohammadi KL. Molecular typing of toxigenic Clostridum perfringens isolated from sheep in Iran. 2011.
  7. Jabbari A, Tekyei F, Esmaelizad M, PILEHCHIAN LR. Occurrence of Beta2 toxigenic Clostridium perfringens isolates with different toxin types in Iran. 2012.
  8. Ezatkhah M, Alimolaei M, Amini M, Shamsaddini Bafti M. Typing Toxigenic Clostridium perfringens Strains from the Ruminants of Yazd Province by Multiplex Polymerase Chain Reaction. Int J Enteric Pathog. 2016;4(3):1-4.
  9. Ahsani M, Bafti MS, Esmailizadeh A, Mohammadabadi M. Genotyping of isolates of Clostridium perfringens from vaccinated and unvaccinated sheep. Small Rumin Res. 2011;95(1):65-9.
  10. Ahsani M, Mohammadabadi M, Shamsaddini M. Clostridium perfringens isolate typing by multiplex PCR. J Venom Anim Toxins Incl Trop Dis. 2010;16(4):573-8.
  11. Uzal FA, Songer JG, Prescott JF, Popoff MR. Clostridial diseases of animals: John Wiley & Sons; 2016.
  12. Shrestha A, Uzal FA, McClane BA. Enterotoxic clostridia: Clostridium perfringens enteric diseases. Microbiol Spectr. 2018;6(5):6.5. 07.
  13. Greco G, Madio A, Buonavoglia D, Totaro M, Corrente M, Martella V, et al. Clostridium perfringens toxin-types in lambs and kids affected with gastroenteric pathologies in Italy. Vet J. 2005;170(3):346-50.
  14. Alimolaei M, Ezatkhah M. Prevalence and genotypic characterization of Clostridium perfringens associated with goat (Capra hircus) enterotoxemia in Southeast Iran. Small Rumin Res. 2022:106805.
  15. Wang R-F, Cao W-W, Campbell WL, Hairston L, Franklin W, Cerniglia CE. The use of PCR to monitor the population abundance of six human intestinal bacterial species in an in vitro semicontinuous culture system. FEMS Microbiol Lett. 1994;124(2):229-37.
  16. Feldman SH, Songer JG, Bueschel D, Weisbroth SP, Weisbroth SH. Multifocal necrotizing enteritis with hepatic and splenic infarction associated with Clostridium perfringens type A in a guinea pig raised in a conventional environment. Comp Med. 1997;47(5):540-4.
  17. Miwa N, Nishina T, Kubo S, Fujikura K. Nested polymerase chain reaction for detection of low levels of enterotoxigenic Clostridium perfringens in animal feces and meat. J Vet Med Sci. 1996;58(3):197-203.
  18. Baums CG, Schotte U, Amtsberg G, Goethe R. Diagnostic multiplex PCR for toxin genotyping of Clostridium perfringens isolates. Vet Microbiol. 2004;100(1):11-6.
  19. Keyburn AL, Boyce JD, Vaz P, Bannam TL, Ford ME, Parker D, et al. NetB, a new toxin that is associated with avian necrotic enteritis caused by Clostridium perfringens. PLoS Pathog. 2008;4(2):e26.
  1. El Idrissi AH, Ward GE. Evaluation of enzyme-linked immunosorbent assay for diagnosis of clostridium perfringens enterotoxemias. Vet Microbiol. 1992;31(4):389-96.
  2. Anju K, Karthik K, Divya V, Priyadharshini MLM, Sharma RK, Manoharan S. Toxinotyping and molecular characterization of antimicrobial resistance in Clostridium perfringens isolated from different sources of livestock and poultry. Anaerobe. 2021;67:102298.
  3. Mohiuddin M, Iqbal Z, Siddique A, Liao S, Salamat MKF, Qi N, et al. Prevalence, genotypic and phenotypic characterization and antibiotic resistance profile of Clostridium perfringens type A and D isolated from feces of sheep (Ovis aries) and goats (Capra hircus) in Punjab, Pakistan. Toxins. 2020;12(10):657.
  4. Forti K, Ferroni L, Pellegrini M, Cruciani D, De Giuseppe A, Crotti S, et al. Molecular characterization of Clostridium perfringens strains isolated in Italy. Toxins. 2020;12(10):650.
  5. Uzal F. Diagnosis of Clostridium perfringens intestinal infections in sheep and goats. Anaerobe. 2004;10(2):135-43.
  6. Harrison B, Raju D, Garmory HS, Brett MM, Titball RW, Sarker MR. Molecular characterization of Clostridium perfringens isolates from humans with sporadic diarrhea: evidence for transcriptional regulation of the beta2-toxin-encoding gene. Appl Environ Microbiol. 2005;71(12):8362-70.
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