Fermentation and formulation of Trichoderma asperellum for biological control of sugar beet damping–off disease | ||
| مهار زیستی در گیاهپزشکی | ||
| Volume 9, Issue 2 - Serial Number 18, March 2022, Pages 59-66 PDF (361.22 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/bcpp.2022.128528 | ||
| Authors | ||
| Asghar Heydari* ; Shahram Naeemi; Laleh Naraghi; Rasoul Marzban; Kobra Golkar | ||
| Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension (AREEO), Tehran, Iran. | ||
| Abstract | ||
| Biological control of plant diseases using bacterial and fungal antagonists has been accepted as a suitable alternative for the chemical control which is harmful for human health and it is also a contaminant of the agricultural environment. One of the important limitations of biocontrol agents is the ineffectiveness in the field conditions which is related to the lack of stability and duration of biocontrol agents that can mostly be due to the lack of proper formulations. In this research study, four new bioformulations were developed and prepared using two strains of a fungal antagonist (Trichoderma asperellum), rice bran as the substrate, Kaolin as the carrier and carboxy methyl cellulose and arabic gum as the stickers. For the development of bioformulations, rice bran substrate was mixed with spore suspension of two most effective strains of T. asperellum . Plastic bags containing rice bran and fungal antagonists spore suspension were incubated for two months at 250C until the fungus covered the whole surface of the substrate in the bags (Fermentation phase). The containing of the bags was then evacuated and the powdery colonized substrate was used for the development of final bioformulations. The bioformulations were developed and produced by mixing Kaolin powder as the carrier by 20% of the substrate weight and two stickers including Carboxy Methyl Cellulose (CMC) and Arabic Gum (AG) each by 1% of substrate weight. Four bioformulations were produced with the identification codes of Ka–CMC–Ta–4, Ka–AG–Ta–4, Ka–CMC–Ta–5 and Ka–AG–Ta–5 respectively. In addition, the shelf life and some important physical and chemical properties of the bioformulations including percent humidity, pH and their suspensibility were also determined. | ||
| Keywords | ||
| Sugar beet; Seedling damping–off; Rhizoctonia solani; Bioformulations; Trichoderma asperellum | ||
| References | ||
|
Ardakani, S., Heydari, A., Khorasani, N. & Arjmandi, R. 2010. Development of new bioformulations of Pseudomonas fluorescens and evaluation of these products against damping–off of cotton seedlings. Journal of Plant Pathology, 92(1): 83–88. Ershad, J. & Shirazi, G. 1997. Sugar beet damping–off disease Iranian Journal of Plant Disease. 35: 243–249. Chet, I., Harman, G.E. & Baker, R. 2007. Trichoderma hamatum: Its Hyphal Interactions with Rhizoctonia solani and Pythium spp Microbial Ecology, 7(1): 29–38. Draycott, A.P. 2006. Sugar beet. Blackwell publishing, 474pp. Herr, L.J. 1996. Sugar beet diseases incited by Rhizoctonia species. In: Rhizoctonia species: Taxonomy, Molecular Biology, Ecology, Pathology and Disease Control, eds. Sneh, B., Jabaji–Hare, S., Neate, S. and Dijst, G. Kluwer Academic Publishers, 341–350. Heydari, A. & Misaghi, I.J. 2003. The role of rhizosphere bacteria in herbicide–mediated increase in Rhizoctonia solani–induced cotton seedling damping–off. Plant & Soil, 257: 391–396. Heydari, A. & Pessarakli, M. 2010. A review on biological control of fungal plant pathogens using microbial antagonists. Journal of Biological Sciences, 10(4): 272–290. Huang, J.W. & Huang, H.C. 2000. A formulated container medium suppressive to Rhizoctonia damping– off of cabbage. Botany Bulletin Academic Scince, 41: 49–56. Jorjani, M., Heydari, A., Zamanizadeh, H.R., Rezaee, S. & Naraghi, L. 2013. Controlling sugar beet mortality disease by application of new bioformulations. Journal of Plant Protection Research, 52(3): 303–307. Kakvan, N., Heydari, A., Zamanizadeh, H., Rezaee, S. & Naraghi, L. 2013. Development of new bioformulations using Trichoderma and Talaromyces fungal antagonists for biological control of sugar beet damping–off disease. Crop Protection, 53: 80–84. Marois, J.J., Fravel, D.R. & Papavizas, G.C. 1984. Ability of Talaromyces flavus to occupy the rhizosphere. Soil Biol. Biochem, 16(4): 387–390. Naeimi, S., Khosravi, V., Nouri, M.Z., Hoda, H., Vágvölgyi, C. & Kredics, L. 2019. Biological control of rice sheath blight disease with formulation of indigenous Trichoderma strains under paddy field condition. Acta Biologica Szegediensis, 63(1): 37–43. Naeimi, S., Khosravi, V., Varga, A., Vágvölgyi, C. & Kredics, L. 2020. Screening of organic substrates for solid–state fermentation, viability and bioefficacy of Trichoderma harzianum AS12–2, a biocontrol strain against rice sheath blight disease. Agronomy, 10(9): 1258. Naraghi, L., Heydari, A., Karimi Roozbehani, A. & Ershad, J. 2003. Isolation of Talaromyces flavus from Gorgan cotton fields and investigation of its antagonistic effects against Verticillium dahlia causal agent of cotton wilt disease. Iranian Journal of Plant Pathology, 39: 109–121. Nikan, J., Heydari, A., Naraghi, L., Arjmandian, A. & Mahdizadeh Naraghi, R. 2018. Application of some fungal bioformulations for controlling garlic white rot disease in the field conditions. Biocontrol in Plant Protection, 5(2): 75–83. Prathap, M. & Kumari, B.D. 2017. Bioformulation in Biological Control for Plant Diseases–A Review. International Journal of Biotech Trends and Technology, 7(3): 1–8. Ramesh, N., Rezaee, S., Naeimi, S. & Fotouhifar, K. 2020. Evaluation of rice fungal endophytes for biological control of blast disease. BioControl in Plant Protection, 8(2): 1–17. Rehn, V.N.C., Rehn, K.G., Mendonça–Hagle, L., Smalla, K. & Berg, K. 2007. Analysis of antagonistic interaction between Trichoderma isolates from Brazilian weeds and the soil–borne pathogen Rhizoctonia solani. Journal of Plant Disease and Protection, 14(4): 167–175. Rouhani, H. Karimi Roozbehani, A. & Khoaparast, F. 1990. The role of Iranian isolates of Trichoderma in biological control of Rhizoctonia solani. Iranian Journal of Plant Pests and Diseases, 58(1–3): 17–35. Yobo, K.S., Laing M.D., Hunter, C.H. & Morris, M.J. 2004. Biological control of Rhizoctonia solani by two Trichoderma species isolated from South African composted soil. South African Journal of Plant Soil, 21(3): 139–145.
| ||
|
Statistics Article View: 647 PDF Download: 462 |
||
Journal management system. designed by sinaweb