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اثر سطوح مختلف نانوذرات نقره، اسیدهیومیک و تنش خشکی بر جوانهزنی بذر و برخی ویژگیهای مورفوفیزیولوژیک دانهرست کینوا رقم Titicaca | ||
| علوم و فناوری بذر ایران | ||
| مقاله 5، دوره 11، شماره 4 - شماره پیاپی 28، آذر 1401، صفحه 65-84 اصل مقاله (1.86 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijsst.2022.358267.1428 | ||
| نویسندگان | ||
| معصومه عامریان* 1؛ محمود خرمی وفا2؛ برکت الله برانی3 | ||
| 1اســتادیار، گــروه مهندســی تولیــد و ژنتیــک گیاهــی، دانشــکده علــوم و مهندســی کشــاورزی، دانشــگاه رازی، کرمانشــاه، ایــران | ||
| 2گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی، دانشگاه رازی، کرمانشاه، ایران | ||
| 3دانشجوی دکتری، گروه تولید و ژنتیک گیاهی، دانشکده علوم و مهندسی کشاورزی، دانشگاه رازی، کرمانشاه، ایران | ||
| چکیده | ||
| در این پژوهش اثر نانوذرات نقره و اسیدهیومیک بر برخی ویژگیهای مرفولوژیک دانهرست کینوا تحت تنش خشکی مورد بررسی قرار گرفت. آزمایش بهصورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار اجرا گردید. آزمایش شامل سه سطح نانوذره نقره (صفر، 10 و 20 میلیگرم بر لیتر نانونقره)، سه سطح اسیدهیومیک (صفر، 100 و 300 میلیگرم بر لیتر) و سه سطح تنش خشکی (صفر، 6- و 12- بار) بود. بیشترین میزان بنیهی بذر و سرعت جوانهزنی بذر در بالاترین سطح نانوذره نقره و اسیدهیومیک مشاهده گردید که نشاندهندهی تأثیر مثبت نانوذره نقره و اسیدهیومیک بر درصد جوانهزنی، سرعت جوانهزنی و بنیهی بذر کینوا است. با توجه به نتایج بهدست آمده برخلاف نانوذرات نقره و اسیدهیومیک تنش خشکی منجربه کاهش درصد جوانهزنی و خصوصیات رشدی دانهرست کینوا شد.کاهش جذب آب توسط بذر در شرایط تنش خشکی سبب کاهش فرایندهای فیزیولوژیکی و متابولیکی بذر میشود. نانوذرات میتوانند به دیواره سلولی نفوذ کنند و منافذ جدیدی را برای نفوذ آب در پوسته بذر ایجاد کنند و به این ترتیب سرعت جوانهزنی را افزایش دهند. با افزایش غلظت نانوذرات نقره و اسیدهیومیک میزان پرولین، قندهای محلول، فنل کل، فلاونوئید و فعالیت آنزیم کاتالاز تحت شرایط تنش خشکی افزایش نشان داد که بیانگر نقش مثبت نانوذرات نقره و اسیدهیومیک برای افزایش تحمل به خشکی در گیاه کینوا است. در نتیجه کاربرد اسیدهیومیک در غلظت 300 میلیگرم بر لیتر همراه با 20 میلیگرم بر لیتر نانوذرات نقره برای بهبود جوانهرنی و رشد دانهرست کینوا تحت شرایط تنش خشکی توصیه میشود. | ||
| کلیدواژهها | ||
| درصد جوانهزنی؛ پلیاتیلن گلایکول؛ پرولین؛ آنزیم کاتالاز؛ میکروسکوپ الکترونی | ||
| عنوان مقاله [English] | ||
| Effect of Different Levels of Silver Nanoparticles, Humic Acid and Drought Stress on Seed Germination and Some Morphophysiological Characteristics of Quinoa Seedlings of Titicaca Cultivar | ||
| نویسندگان [English] | ||
| Masoomeh Amerian1؛ Mahmoud Khoramivafa2؛ Barakat Alah Rabani3 | ||
| 1Assistant Professor, Department of Production Engineering and Plant Genetics, Faculty of Science and Agriculture Engineering, Razi University, Kermanshah, Iran | ||
| 2Department of agronomy and plant breeding, campus of agriculture and natural resources, Razi university, Kermansha, Iran | ||
| 3Ph.D student, Department of Production Engineering and Plant Genetics, Faculty of Science and Agriculture Engineering, Razi University, Kermanshah, Iran | ||
| چکیده [English] | ||
| in this study, the effect of silver nanoparticles and humic acid on some morphological characteristics of quinoa seedlings under drought stress was investigated. The experiment was performed as a factorial experiment in a completely randomized design with three replications. The experiment consisted of three levels of silver nanoparticles (0, 10 and 20 mg L-1 AgNPs), three levels of humic acid (0, 100 and 300 mg L-1) and three levels of drought stress (0, -6 and -12 bar). In this study, Titicaca cultivar was used. The highest amount of seed vigor and seed germination rate were observed in the highest level of AgNPs and humic acid, which indicates the positive effect of AgNPs and humic acid on germination percentage, germination rate and quinoa seed vigor. According to the obtained results, drought stress led to a decrease in germination percentage and growth characteristics of quinoa seeds. AgNPs and humic acid had a positive effect on germination and growth characteristics of quinoa seedlings under drought stress conditions compared to control treatment. With increasing the concentration of AgNPs and humic acid, the amount of proline, soluble sugars, total phenol, flavonoids and catalase activity under drought stress increased, indicating the positive role of AgNPs and humic acid to increase drought tolerance in quinoa. Therefore, the use of humic acid at a concentration of 300 mg L-1 along with 20 mg L-1 AgNPs is recommended to improve germination and seed growth of quinoa under drought stress conditions | ||
| کلیدواژهها [English] | ||
| : Proline, Enzyme catalase, Germination percentage, Polyethylene glycol, Electron microscope | ||
| مراجع | ||
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