Zokaei, L, Akbari, E, Babapour, V, Zendehdel, M. (1402). The Modulatory Role of Orexin 1 Receptor in Nucleus Accumbens (NAc) on Spatial Memory in Rats. سامانه مدیریت نشریات علمی, 78(4), 1285-1294. doi: 10.32592/ARI.2023.78.4.1285
L Zokaei; E Akbari; V Babapour; M Zendehdel. "The Modulatory Role of Orexin 1 Receptor in Nucleus Accumbens (NAc) on Spatial Memory in Rats". سامانه مدیریت نشریات علمی, 78, 4, 1402, 1285-1294. doi: 10.32592/ARI.2023.78.4.1285
Zokaei, L, Akbari, E, Babapour, V, Zendehdel, M. (1402). 'The Modulatory Role of Orexin 1 Receptor in Nucleus Accumbens (NAc) on Spatial Memory in Rats', سامانه مدیریت نشریات علمی, 78(4), pp. 1285-1294. doi: 10.32592/ARI.2023.78.4.1285
Zokaei, L, Akbari, E, Babapour, V, Zendehdel, M. The Modulatory Role of Orexin 1 Receptor in Nucleus Accumbens (NAc) on Spatial Memory in Rats. سامانه مدیریت نشریات علمی, 1402; 78(4): 1285-1294. doi: 10.32592/ARI.2023.78.4.1285
The Modulatory Role of Orexin 1 Receptor in Nucleus Accumbens (NAc) on Spatial Memory in Rats
1Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
2Department of Physiology and Pharmacology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
3Immunogenetics Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
4Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, 14155-6453, Tehran, Iran
چکیده
Neuropeptide orexin mainly exists in neurons within and around the lateral hypothalamus and exhibits high affinity to orexin 1 and 2 receptors (OX1R and OX2R, respectively). Orexinergic neurons send their axons to the nucleus accumbens (NAc), which expresses OX1Rs. Previous studies have shown the involvement of orexins and their role in learning and memory processes in the dorsal raphe nucleus and hippocampus. However, no study has examined the effects of orexins in the NAc on memory. The present study examined the effect of the post-training and pre-probe trial intra-NAc administration of SB-33486-A (OX1R antagonist, 12 μg/0.5μl) and TCS-OX2-29 (OX2R antagonist, 10 μg/0.5 μl) on the consolidation and retrieval of memory in the Morris Water Maze (MWM) task. In experiment 1, rats were trained in the MWM and, immediately after every training, received bilateral injections of dimethyl sulfoxide (DMSO) (control group), SB-334867-A (SB), and TCS-OX2-29 (TCS) into the NAc. Experiment 2 was similar to experiment 1, except that the rats received DMSO, SB, and TCS 15 min before the probe test. Probe and visible tests were performed after the last training, and the distance moved, escape latency, and velocity were recorded. In experiment 3,rats trained in experiments 1 and 2, immediately after the probe test, were given the trials for visuomotor coordination assessment on the visible platform. According to the results, the injection of SB increased the distance moved and escape latency in the treated groups, compared to the control group, in the consolidation phase of spatial memory (P<0.05) but not in its retrieval phase (P>0.05). However, TCS-OX2-29 had no effect. These results suggest that the inactivation of the NAc OX1Rs, but not OX2Rs, impairs the consolidation but not the retrieval of spatial memory in rats.
Alijanpour S, Tirgar F, Zarrindast M-R. Role of dorsal hippocampal orexin-1 receptors in memory restoration induced by morphine sensitization phenomenon. Neuroscience. 2016;312:215-26.
Han D, Han F, Shi Y, Zheng S, Wen L. Mechanisms of memory impairment induced by orexin-A via orexin 1 and orexin 2 receptors in post-traumatic stress disorder rats. Neuroscience. 2020;432:126-36.
Rahmani B, Ghashghayi E, Zendehdel M, Khodadadi M, Hamidi B. The crosstalk between brain mediators regulating food intake behavior in birds: a review. Int J Pept Res Ther 2021;27(4):2349-70.
Resae A, Yousefi M, Naeimi S, Mahdavi A. Effects of Occupational Formaldehyde Exposure on Passive Avoidance Conditioning and Anxiety Levels in Wistar rats. Iran J Vet Med. 2022.
Akbari E, Naghdi N, Motamedi F. Functional inactivation of orexin 1 receptors in CA1 region impairs acquisition, consolidation and retrieval in Morris water maze task. Behav Brain Res. 2006;173(1):47-52.
Akbari E, Motamedi F, Naghdi N, Noorbakhshnia M. The effect of antagonization of orexin 1 receptors in CA1 and dentate gyrus regions on memory processing in passive avoidance task. Behav Brain Res. 2008;187(1):172-7.
Zhao X, xue Zhang R, Tang S, yan Ren Y, xia Yang W, min Liu X, et al. Orexin-A-induced ERK1/2 activation reverses impaired spatial learning and memory in pentylenetetrazol-kindled rats via OX1R-mediated hippocampal neurogenesis. Peptides. 2014;54:140-7.
García-Brito S, Aldavert-Vera L, Huguet G, Álvarez A, Kádár E, Segura-Torres P. Increased training compensates for OX1R blockage-impairment of spatial memory and c-Fos expression in different cortical and subcortical areas. Behav Brain Res. 2018;353:21-31.
Akbari E, Motamedi F, Davoodi FG, Noorbakhshnia M, Ghanbarian E. Orexin-1 receptor mediates long-term potentiation in the dentate gyrus area of freely moving rats. Behav Brain Res. 2011;216(1):375-80.
Dang R, Chen Q, Song J, He C, Zhang J, Xia J, et al. Orexin knockout mice exhibit impaired spatial working memory. Neurosci Lett. 2018;668:92-7.
Thorpe A, Kotz C. Orexin A in the nucleus accumbens stimulates feeding and locomotor activity. Brain research. 2005;1050(1-2):156-62.
Li B, Chang L, Peng X. Orexin 2 receptor in the nucleus accumbens is critical for the modulation of acute stress-induced anxiety. Psychoneuroendocrinology. 2021;131:105317.
Paxinos G. Paxinos & Watson the Rat Brain in Stereotaxic Coordinates: The New Coronal Set: Elsevier; 2005.
Akbari E, Naghdi N, Motamedi F. The selective orexin 1 receptor antagonist SB-334867-A impairs acquisition and consolidation but not retrieval of spatial memory in Morris water maze. Peptides. 2007;28(3):650-6.
Khodabande F, Akbari E, Ardeshiri MR. The modulation of the spatial reference memory by the orexinergic system of the dorsal raphe nucleus. Life Sci. 2021;265:118777.
Mavanji V, Butterick TA, Duffy CM, Nixon JP, Billington CJ, Kotz CM. Orexin/hypocretin treatment restores hippocampal-dependent memory in orexin-deficient mice. Neurobiol Learn Mem. 2017;146:21-30.
Yang L, Zou B, Xiong X, Pascual C, Xie J, Malik A, et al. Hypocretin/orexin neurons contribute to hippocampus-dependent social memory and synaptic plasticity in mice. J Neurosci. 2013;33(12):5275-84.
Selbach O, Bohla C, Barbara A, Doreulee N, Eriksson K, Sergeeva O, et al. Orexins/hypocretins control bistability of hippocampal long‐term synaptic plasticity through co‐activation of multiple kinases. Acta physiologica. 2010;198(3):277-85.
19. Guo Y, Feng P. OX2R activation induces PKC-mediated ERK and CREB phosphorylation. Exp Cell Res. 2012;318(16):2004-13.
