Combined effect of lactoferrin and nanobody loaded on chitosan against Staphylococcus aureus and Pseudomonas aeruginosa | ||
| علوم و فنون دامپروری | ||
| Volume 11, Issue 44, January 0, Pages 7-16 PDF (1.58 M) | ||
| DOI: 10.22092/aasrj.2022.128151 | ||
| Authors | ||
| Sh. Soleimani; S. Zibaei* | ||
| Abstract | ||
| Lactoferrin has a molecular weight of about 80 kDa and a globular glycoprotein. After isolation from camel milk, lactoferrin was purified using CM Sephadex C-50 ion exchange chromatography. By injecting two bacteria, Staphylococcus aureus and Pseudomonas aeruginosa, with chitosan and alum adjuvants to camel. Nanobodies were prepared and the production of specific antibodies was confirmed using ELISA. Lactoferrin and nanobody were loaded on chitosan using thiamine pyrophosphate method. The combined effect of lactoferrin and nanobody loaded on chitosan against bacteria was investigated using a bacterial growth test in the presence or absence (control) of the sample. For statistical analysis, first using Prism graph pad software 9 one-way analysis of variance was performed and if there was a significant difference of P <0.05, Tukey test was used to separate the groups. Based on the results, depending on the concentration of lactoferrin and nanobody dilution, the growth rate of Staphylococcus aureus and Pseudomonas aeruginosa decreased by 57-77.5% and 51-53.5%, respectively, compared to the control group. It was stated that lactoferrin and nanobody loaded on chitosan are more effective on gram-positive bacteria than gram-negative bacteria. Based on the results, it can be stated that lactoferrin and nanobody loaded on chitosan as an anti-Bacteria can be useful in a variety of commercial programs to control the growth and survival of pathogens. | ||
| Keywords | ||
| lactoferrin; aeruginosa; Staphylococcus aureus; Pseudomonas aeruginosa | ||
| References | ||
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