| تعداد نشریات | 286 |
| تعداد نشریات سازمان | 84 |
| تعداد شماره‌ها | 2,975 |
| تعداد مقالات | 33,408 |
| تعداد مشاهده مقاله | 43,902,696 |
| تعداد دریافت فایل اصل مقاله | 20,405,028 |
Macrophomina vaccinii a new species for funga of Iran | ||
| Mycologia Iranica | ||
| مقاله 5، دوره 9، شماره 1، شهریور 2022، صفحه 51-57 اصل مقاله (1.2 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22092/MI.2022.360409.1238 | ||
| نویسنده | ||
| Fariba Ghaderi* | ||
| Assistant Professor, Department of Plant Protection, College of Agriculture, Yasouj University, Iran | ||
| چکیده | ||
| In summer 2020, bean plants (Phaseolus vulgaris L.) with symptoms of wilting, chlorosis, drying and the formation of numerous microsclerotia in the stem were detected from Ghachsaran fields, Kohgiluyeh & Boyer-Ahmad provinces of Iran. Infected samples of stem were taken to the laboratory, cut into small sections, sterilized, washed in sterile water, dried on sterile paper towels, and cultured on Potato Dextrose Agar (PDA) amended with 50 µg of kanamycin to prevent bacterial contamination. Isolates were purified by hyphal tip technique. Four isolates from infected stems tissue were recovered. Fungal isolates were identified based on morphological characteristics and molecular data of tef1-α gene. According to the morphological and phylogenetic analysis, the isolates were identified as Macrophomina vaccinii. This is the first report of M. vaccinii, in Iran | ||
| کلیدواژه‌ها | ||
| Common bean؛ Iran؛ morphology؛ phylogeny؛ tef1-α | ||
| مراجع | ||
|
Carbone I, Kohn LM. 1999. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycology 91: 553–556.
Cohen R, Elkabetz M, Edelstein M. 2016. Variation in the responses of melon and watermelon to Macrophomina phaseolina. Crop Protection 85: 46–51.
Crous PW, Slippers B, Wingfield MJ, Rheeder J, Marasas WFO, et al. 2006. Phylogenetic lineages in the Botryosphaeriaceae. Studies in Mycology 55: 235–253.
Dasgupta MK. Principles of Plant Pathology. Allied Publishers Pvt. Ltd., Banglore, 1988, 1140.
Edraki V, Banihashemi Z. 2010. Phenotypic diversity among isolates of Macrophomina phaseolina and its relation to pathogenicity. Iranian Journal of Plant Pathology 46(4): 93-100.
Ershad D. 2022. Fungi anf fungal analogues of Iran, Iranian Research Institution of Plant Protection, Tehran, Iran.
Farr DF, Rossman AY .2020. Fungal databases, systematic mycology and microbiology laboratory. ARS, USDA, Washington, DC.
Gupta GK, Sharma SK, Ramteke R. 2012. Biology, epidemiology and management of the pathogenic fungus Macrophomina phaseolina (Tassi) Goid with special reference to charcoal rot of soybean (Glycine max (L.) Merrill). Journal of Phytopathology 160: 167-180.
Hall T. 2012. BioEdit v7.0.9: Biological sequence alignment editor for Win95/98/2K/XP/7. http://www.mbio.ncsu. edu/bioedit/bioedit.html.
Jacobs K, Bergdahl DR, Wingfield MJ, Halik S, Seifert KA, et al. 2004. Leptographium wingfieldii introduced into North America and found associated with exotic Tomicus piniperda and native bark beetles. Mycological Research 108: 411–418.
Katoh K, Standley DM. 2013. MAFFT Multiple Sequence Alignment Software Version 7: improvements in performance and usability. Molecular Biology and Evolution 30: 772–780.
Machado AR, Pinho DB, Soares DJ, Gomes AAM Pereira OL. 2018. Bayesian analyses of five gene regions reveal a new phylogenetic species of Macrophomina associated with charcoal rot on oilseed crops in Brazil. European Journal of Plant Pathology 153: 89–100.
Mayek-PÉrez N, GarcÍa-Espinosa R, López-Castañeda C. 2002. Water relations, histopathology and growth of common bean (Phaseolus vulgaris L.) during pathogenesis of Macrophomina phaseolina under drought stress. Physiological and Molecular Plant Pathology 60(4):185-195.
Melo JF, Brito ACQ, Vieira JCB, Câmara MPS, Michereff SJ, Motta CS, Machado AR. 2021. Identification and pathogenicity of Botryosphaeriaceae species associated with root and stem rot of sweet potato in Brazil. Plant Pathology 70(7): 1–15.
Murray M, Thompson, WF. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8: 4321–4325.
Osdaghi E and Lak MR. 2015. A source of resistance to bacterial wilt in the common bean (Phaseolus vulgaris) in Iran. Crop Protection 74: 37-41.
Phillips AJL, Alves A, Abdollahzadeh J, Slippers B, Wingfield MJ, Groenewald JZ, Crous, PW. 2013. The Botryosphaeriaceae: genera and species known from culture. Studies in Mycology 76: 51–167.
Pickel B, Dai N, Maymon M, Elazar M, Tanami Z, Frenkel O, Toamy MA, Mor N, Freeman S. 2020. Development of a reliable screening technique for determining tolerance to 700 Macrophomina phaseolina in strawberry. European Journal of Plant Pathology 157: 707-718.
Poudel B, Shivas RG, Adorada DL, Barbetti MJ, Bithell SL, Kelly LA, Moore N, et al (2021). Hidden diversity of Macrophomina associated with broadacre and horticultural crops in Australia. European Journal of Plant Pathology 161: 1–23.
Sarr MP, Ndiaye M, Groenewald JZ, Crous PW. 2014. Genetic diversity in, Macrophomina phaseolina, the causal agent of charcoal rot. Phytopathologia Mediterranea 53(2): 250– 268.
Zhao L, Cai J, He W, Zhang Y. 2019. Macrophomina vaccinii sp. nov. causing blueberry stem blight in China. MycoKeys 55: 1–14. | ||
|
آمار تعداد مشاهده مقاله: 384 تعداد دریافت فایل اصل مقاله: 408 |
||