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Al Talebi, Z, Karhib, M. M, Taki, M. M, Salaam Abood, E, Mubarak, H. A, Sahar Ahmed Ibrahim, C. (1402). Chemical Analysis, Antioxidant, and Antibacterial Potential of Aqueous Extract of Broccoli (Brassica oleracea) Using GC-ms. سامانه مدیریت نشریات علمی, 78(2), 593-599. doi: 10.22092/ari.2022.359860.2488
Z Al Talebi; M. M Karhib; M. M Taki; E Salaam Abood; H. A Mubarak; C Sahar Ahmed Ibrahim. "Chemical Analysis, Antioxidant, and Antibacterial Potential of Aqueous Extract of Broccoli (Brassica oleracea) Using GC-ms". سامانه مدیریت نشریات علمی, 78, 2, 1402, 593-599. doi: 10.22092/ari.2022.359860.2488
Al Talebi, Z, Karhib, M. M, Taki, M. M, Salaam Abood, E, Mubarak, H. A, Sahar Ahmed Ibrahim, C. (1402). 'Chemical Analysis, Antioxidant, and Antibacterial Potential of Aqueous Extract of Broccoli (Brassica oleracea) Using GC-ms', سامانه مدیریت نشریات علمی, 78(2), pp. 593-599. doi: 10.22092/ari.2022.359860.2488
Al Talebi, Z, Karhib, M. M, Taki, M. M, Salaam Abood, E, Mubarak, H. A, Sahar Ahmed Ibrahim, C. Chemical Analysis, Antioxidant, and Antibacterial Potential of Aqueous Extract of Broccoli (Brassica oleracea) Using GC-ms. سامانه مدیریت نشریات علمی, 1402; 78(2): 593-599. doi: 10.22092/ari.2022.359860.2488

Chemical Analysis, Antioxidant, and Antibacterial Potential of Aqueous Extract of Broccoli (Brassica oleracea) Using GC-ms

Archives of Razi Institute
مقاله 12، دوره 78، شماره 2، خرداد و تیر 2023، صفحه 593-599    اصل مقاله (625.93 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359860.2488
نویسندگان
Z Al Talebi* 1؛ M. M Karhib2؛ M. M Taki3؛ E Salaam Abood4؛ H. A Mubarak5؛ C Sahar Ahmed Ibrahim6
1Chemistry Department, College of Science, University of Babylon, Babylon, Iraq
2Department of Medical Laboratory Techniques, Al-Mustaqbal University College, 51001 Hillah, Babylon, Iraq
3Nanotechnology and Advanced Materials Research Centre, University of Technology, Baghdad, Iraq
4Medical Physics Department, Hilla University College, Babylon, Iraq
5Department of Chemical Engineering, College of Engineering, University of Babylon, Babylon, Iraq
6Babil Teaching Hospital For Maternity and Children, Babylon, Iraq
چکیده
Antioxidant and antibacterial chemicals are key sources in medicinal plants. Alkaloids, phenolics, steroids, terpenes, flavonoids, terpenes, and volatile oils are a few of these plants' secondary metabolites. Phytochemicals, particularly the secondary metabolites produced by plants, are important for human nutrition, well-being, illness prevention, and antibacterial properties. This study aimed to ascertain the aqueous broccoli extract's chemical makeup. The phytochemical molecule that the GC-MS technique identified. The DPPH assay, which is appropriate for regular plant material screening, was performed to assess the antioxidant capacities of broccoli extract (in vitro). Subsequently looks at how well they perform against different Gram-positive and Gram-negative harmful microorganisms. GC-MS analysis of Broccoli extract revealed the existence of the9-Octadecenamide, [C18H35O], Hexadecane [c16h34] and 2, 2, 2-trifluoroethyl 2-methyltetrahydro-5-oxo-3- furancarboxylate [C23H33NO6]. There were significant changes in the extract's ascorbic acid-free radical scavenging activity at 200, 100, and 25 g/ml (P 0.05), and the activity was dose-dependent. The effectiveness of aqueous broccoli extract as a powerful, broad-spectrum antibacterial agent against tested bacteria is demonstrated by an increase in the diameter of the inhibition zone, which increases in direct proportion to the concentration of the extract and even surpasses the activity of some antibiotics. An appropriate concentration of aqueous broccoli extract strongly inhibits microbial and antioxidant growth, especially when treating external infections without any danger against resistant bacterial isolates; it is strongly advised to use aqueous broccoli extract as a cost-effective alternative antibacterial and antioxidant agent.
کلیدواژه‌ها
Aqueous Broccoli Extract؛ GC-MS؛ Antioxidant؛ Antibacterial
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مراجع
  1. Hu SH, Wang JC, Kung HF, Wang JT, Lee WL, Yang YH. Antimicrobial effect of extracts of cruciferous vegetables. Kaohsiung J Med Sci. 2004;20(12):591-9.
  2. Mahn A, Reyes A. An overview of health-promoting compounds of broccoli (Brassica oleracea var. italica) and the effect of processing. Food Sci Technol Int. 2012;18(6):503-14.
  3. Owis AI. Broccoli: The Green Beauty: a review. J Pharm Sci Res. 2015;7(9):696-703.
  4. Pacheco-Cano RD, Salcedo-Hernandez R, Lopez-Meza JE, Bideshi DK, Barboza-Corona JE. Antimicrobial activity of broccoli (Brassica oleracea var. italica) cultivar Avenger against pathogenic bacteria, phytopathogenic filamentous fungi and yeast. J Appl Microbiol. 2018;124(1):126-35.
  5. Park SK, Ha JS, Kim JM, Kang JY, Lee du S, Guo TJ, et al. Antiamnesic Effect of Broccoli (Brassica oleracea var. italica) Leaves on Amyloid Beta (Abeta)1-42-Induced Learning and Memory Impairment. J Agric Food Chem. 2016;64(17):3353-61.
  6. Vinha AF, Alves RC, Barreira SV, Costa AS, Oliveira MB. Impact of boiling on phytochemicals and antioxidant activity of green vegetables consumed in the Mediterranean diet. Food Funct. 2015;6(4):1157-63.
  7. Yang Y, Zhang X. In vitro antitumor activity of broccolini seeds extracts. Scanning. 2011;33(6):402-4.
  8. Vinodh K, Natarajan A, Devi K, Senthilkumar B. Chemical composition of aqueous leaf extract of Murraya Koenigii. Int J Pharm Biol Archiv. 2013;4:493-7.
  9. Gani A. Chemical constituents and uses, medicinal plants of Bangladesh. Asiat Soc Bangladesh. 2003;434.
  10. Fernie AR, Trethewey RN, Krotzky AJ, Willmitzer L. Metabolite profiling: from diagnostics to systems biology. Nat Rev Mol Cell Biol. 2004;5(9):763-9.
  11. Kinnula VL, Crapo JD. Superoxide dismutases in malignant cells and human tumors. Free Radic Biol Med. 2004;36(6):718-44.
  12. Sas K, Robotka H, Toldi J, Vecsei L. Mitochondria, metabolic disturbances, oxidative stress and the kynurenine system, with focus on neurodegenerative disorders. J Neurol Sci. 2007;257(1-2):221-39.
  13. Singh U, Jialal I. Oxidative stress and atherosclerosis. Pathophysiology. 2006;13(3):129-42.
  14. Arteel GE. Oxidants and antioxidants in alcohol-induced liver disease. Gastroenterology. 2003;124(3):778-90.
  15. Bolton JL, Trush MA, Penning TM, Dryhurst G, Monks TJ. Role of quinones in toxicology. Chem Res Toxicol. 2000;13(3):135-60.
  16. Guidi I, Galimberti D, Lonati S, Novembrino C, Bamonti F, Tiriticco M, et al. Oxidative imbalance in patients with mild cognitive impairment and Alzheimer's disease. Neurobiol Aging. 2006;27(2):262-9.
  17. Hyun DH, Hernandez JO, Mattson MP, de Cabo R. The plasma membrane redox system in aging. Ageing Res Rev. 2006;5(2):209-20.
  18. Martysiak-Żurowska D, Wenta W. A comparison of ABTS and DPPH methods for assessing the total antioxidant capacity of human milk. Acta Sci Pol Technol Aliment. 2012;11(1):83-9.
  19. Ramakrishna BS, Varghese R, Jayakumar S, Mathan M, Balasubramanian KA. Circulating antioxidants in ulcerative colitis and their relationship to disease severity and activity. J Gastroenterol Hepatol. 1997;12(7):490-4.
  20. Rimm EB, Ascherio A, Giovannucci E, Spiegelman D, Stampfer MJ, Willett WC. Vegetable, fruit, and cereal fiber intake and risk of coronary heart disease among men. JAMA. 1996;275(6):447-51.
  21. Rimm EB, Katan MB, Ascherio A, Stampfer MJ, Willett WC. Relation between intake of flavonoids and risk for coronary heart disease in male health professionals. Ann Intern Med. 1996;125(5):384-9.
  22. Smith MA, Rottkamp CA, Nunomura A, Raina AK, Perry G. Oxidative stress in Alzheimer’s disease. Biochim Biophys Acta Mol Basis Dis Bba-Mol Basis Dis. 2000;1502(1):139-44.
  23. Upston JM, Kritharides L, Stocker R. The role of vitamin E in atherosclerosis. Prog Lipid Res. 2003;42(5):405-22.
  24. Eberhardt MV, Lee CY, Liu RH. Antioxidant activity of fresh apples. Nature. 2000;405(6789):903-4.
  25. Ganesan K, Kumar KS, Rao PVS. Comparative assessment of antioxidant activity in three edible species of green seaweed, Enteromorpha from Okha, Northwest coast of India. Innov Food Sci Emerg Technol. 2011;12(1):73-8.
  26. Michaud DS, Spiegelman D, Clinton SK, Rimm EB, Willett WC, Giovannucci EL. Fruit and vegetable intake and incidence of bladder cancer in a male prospective cohort. J Natl Cancer Inst. 1999;91(7):605-13.
  27. Rose P, Huang Q, Ong CN, Whiteman M. Broccoli and watercress suppress matrix metalloproteinase-9 activity and invasiveness of human MDA-MB-231 breast cancer cells. Toxicol Appl Pharmacol. 2005;209(2):105-13.
  28. Singh AV, Xiao D, Lew KL, Dhir R, Singh SV. Sulforaphane induces caspase-mediated apoptosis in cultured PC-3 human prostate cancer cells and retards growth of PC-3 xenografts in vivo. Carcinogenesis. 2004;25(1):83-90.
  29. Sivakumar S. Therapeutic potential of chitosan nanoparticles as antibiotic delivery system: challenges to treat multiple drug resistance. Asian J Pharm. 2016;10(2).
  30. Dhandevi P, Jeewon R. Fruit and vegetable intake: Benefits and progress of nutrition education interventions-narrative review article. Iran J Public Health. 2015;44(10):1309.
  31. Liu RH. Dietary bioactive compounds and their health implications. J Food Sci. 2013;78(1):18-25.
  32. Murphy MM, Barraj LM, Spungen JH, Herman DR, Randolph RK. Global assessment of select phytonutrient intakes by level of fruit and vegetable consumption. Br J Nutr. 2014;112(6):1004-18.
  33. Chaudhary A, Rampal G, Sharma U, Thind TS, Singh B, Vig AP, et al. Anticancer, antioxidant activities and GC-MS analysis of glucosinolates in two cultivars of broccoli. Med Chem Drug Disc. 2012;2(2):30-7.
  34. Abdulazeem L, AL-Amiedi BH, Alrubaei HA, AL-Mawlah YH. Titanium dioxide nanoparticles as antibacterial agents against some pathogenic bacteria. Drug Invent Today. 2019;12(5):963-7.
  1. CLSI. Performance standards for Antimicrobial Susceptibility Testing Texas: Clinical and Laboratory Standards Institute: CLSI Document M02-A10 andM07-A8; 2012.
  2. CLSI. Performance Standards for Antimicrobial SusceptibilityTesting Wayne, PA: Clinical and Laboratory Standards Institute: CLSI Supplement M100S; 2020.
  3. Abdulazeem L, Al-Jassani M, Al-Sheakh M. Free Radical Scavenging and Antioxidant Activity of Silver Nanoparticles Synthesized from Cuminum cyminum (Cumin) seed Extract. Res J Pharm Technol. 2021:4349-54.
  4. Wang W, Chen Q, Jiang C, Yang D, Liu X, Xu S. One-step synthesis of biocompatible gold nanoparticles using gallic acid in the presence of poly-(N-vinyl-2-pyrrolidone). Colloids Surf A: Physicochem Eng Asp. 2007;301(1):73-9.
  5. Awika JM, Rooney LW, Wu X, Prior RL, Cisneros-Zevallos L. Screening methods to measure antioxidant activity of sorghum (sorghum bicolor) and sorghum products. J Agric Food Chem. 2003;51(23):6657-62.
  6. Chang ST, Wu JH, Wang SY, Kang PL, Yang NS, Shyur LF. Antioxidant activity of extracts from Acacia confusa bark and heartwood. J Agric Food Chem. 2001;49(7):3420-4.
  7. Kim S-C, Lee D-K. Preparation of TiO2-coated hollow glass beads and their application to the control of algal growth in eutrophic water. Microchem J. 2005;80(2):227-32.
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