- همه نشریات
- نشریات علمی سازمان تحقیقات، آموزش و ترویچ کشاورزی
- مجلات ترویجی سازمان تحقیقات، آموزش و ترویج کشاورزی
- سایر نشریات سازمان تحقیقات، آموزش و ترویج کشاورزی
- نشریات علمی انجمن ها
- علوم آب و خاک
- علوم اقتصاد و ترویج کشاورزی
- علوم جنگل, مرتع و آبخیزداری
- علوم دامی و شیلات
- علوم زراعی و باغی
- علوم گیاهپزشکی
- علوم مهندسی و مکانیزاسیون کشاورزی
- نشریات با زبان انگلیسی
- نشریات با زبان فارسی
- نشریات دانشگاه آزاد اسلامی
- نشریات علمی
پیوندهای مفید
اخبار و اعلانات
آمار
| تعداد نشریات | 281 |
| تعداد نشریات سازمان | 80 |
| تعداد شماره‌ها | 2,458 |
| تعداد مقالات | 28,410 |
| تعداد مشاهده مقاله | 16,616,447 |
| تعداد دریافت فایل اصل مقاله | 12,861,167 |
A new technique for human sperm cryopreservation using emptied sheep’s ovarian follicles | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 16 شهریور 1401 | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2022.359719.2460 | ||
| نویسندگان | ||
Ahmed Hussein Zwamel  1؛ Muhammad-Baqir Muhammad-Rashad Fakhrildin2؛ Haifa Hadi Hassani3
| ||
| 1Radiology Techniques Department, College of Medical Technology, The Islamic University, Najaf, Iraq. | ||
| 2Department of Medical Physiology, Faculty of Medicine, Jabir Ibn Hayyan Medical University, Najaf, Iraq. | ||
| 3Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq. | ||
| چکیده | ||
| Human sperm cryopreservation is a technique used routinely in Assisted reproductive technologies (ART) in order to preserve male fertility in cases where the patient is undergoing a procedure that may affect fertility, such as treatment with chemo- or radiotherapy, vasectomy, and in case of sperm donor to prevent the transition of infectious diseases. The aim of this study was to investigate the possible effects of emptied sheep’s ovarian follicles as a container for cryopreservation of human spermatozoa. Thirty semen samples from oligozoospermic patients and 10 samples from normozoospermic men were used. They were diagnosed according to the standard criteria of WHO 2010.Semen samples classified into four groups (G1-G4) according to sperm concentration: (3-5 million/mL); (6-10 million/mL); (11-15 million/mL) and (16-20 million/mL) respectively. Each sample is divided into two equal parts. One part was cryopreserved without cryoprotectant, while the other was diluted 1:1 with 10% glycerol-based cryosolution. Sheep’s ovarian follicles were obtained from a local slaughterhouse and prepared by slicing the ovaries and evacuating the follicular fluid and oocyte. The emptied follicles were injected with the prepared semen samples. After cryopreservation and thawing, the semen mixture aspired outside the follicles, and sperm parameters: (concentration, progressive motility, total motility, and normal morphology) were measured. Sperm concentration, progressive, and total sperm motility significantly (P<0.01) decreased post-thawing compared with pre-freezing in all groups. In samples cryopreserved without cryoprotectant, sperm concentration was significantly (P<0.01) higher than in those cryopreserved with glycerol, while progressive and total motility were significantly (P<0.01) higher in samples cryopreserved with glycerol than in those cryopreserved without cryoprotectant in all groups—no significant difference found in normal morphology between pre-freezing and post-thawing. Emptied ovarian follicles are an appropriate carrier for cryopreservation of human sperms, especially for patients with oligozoospermia. This technique's best sperm survival rate was observed when using glycerol-based cryosolution. | ||
| کلیدواژه‌ها | ||
| ART؛ Cryopreservation؛ Human spermatozoa؛ Ovarian follicles؛ Sperm cryopreservation | ||
|
آمار تعداد مشاهده مقاله: 83 |
||
سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب