Abbaszadeh, P., Rahmani, H., Saleh-Rastin, N., Khavazi, K., Soltani, A. (2009). Evaluation of Auxin Production From Fluorescent Pseudomonads and Their Effects on Growth of Canola (Brassica napus L.). , 22(2), 203-215. doi: 10.22092/ijsr.2009.126999
P. Abbaszadeh; H. A. Rahmani; N. Saleh-Rastin; K. Khavazi; A. Soltani. "Evaluation of Auxin Production From Fluorescent Pseudomonads and Their Effects on Growth of Canola (Brassica napus L.)". , 22, 2, 2009, 203-215. doi: 10.22092/ijsr.2009.126999
Abbaszadeh, P., Rahmani, H., Saleh-Rastin, N., Khavazi, K., Soltani, A. (2009). 'Evaluation of Auxin Production From Fluorescent Pseudomonads and Their Effects on Growth of Canola (Brassica napus L.)', , 22(2), pp. 203-215. doi: 10.22092/ijsr.2009.126999
Abbaszadeh, P., Rahmani, H., Saleh-Rastin, N., Khavazi, K., Soltani, A. Evaluation of Auxin Production From Fluorescent Pseudomonads and Their Effects on Growth of Canola (Brassica napus L.). , 2009; 22(2): 203-215. doi: 10.22092/ijsr.2009.126999

Evaluation of Auxin Production From Fluorescent Pseudomonads and Their Effects on Growth of Canola (Brassica napus L.)

پژوهش های خاک
Volume 22, Issue 2, February 2009, Pages 203-215    PDF (281.87 K)
Document Type: Research Paper
DOI: 10.22092/ijsr.2009.126999
Authors
P. Abbaszadeh* 1; H. A. Rahmani2; N. Saleh-Rastin3; K. Khavazi2; A. Soltani4
1Graduate Student, Tehran University
2Assistant Professor, Soil and Water Research Institute
3Associate Professor, Tehran University
4Former Graduate Student, Tehran University
Abstract
For assessment of auxin production from fluorescent Pseudomonads and effects of their inoculation on growth of canola seedlings, a study was conducted using 40 strains of Pseudomonads. Fifteen strains had been originally isolated from rhizosphere of wheat as well as two other strains GRP3 and MPFM were obtained from microbial culture collection of Soil and Water Research Institute (SWRI). Twenty – three strains were isolated from rhizosphere soil samples of canola fields from Karaj region. Bacterial strains were isolated by soil serial dilation culture technique on king B agar plates and were identified using morphological and biochemical tests. Two media, enriched TBS and DF minimal salt supplemented with 0, 50, 100 and 200 mg.l-1 of L-TRP were employed to evaluate the efficiency of strains for production of auxin. The strains were further tested for their effects on canola growth in a sand-based pot experiment. Results revealed that auxin biosynthesis was enhanced as L-TRP concentration was increased in culture medium. Strain P23 was the most effective in auxin production in DF medium supplemented with 200 mg.l-1 of L-TRP. Bacterial strains significantly increased growth of canola.The highest correlation was observed between auxin production in DF minimal salt medium with 50 mg.l-1 of L-TRP and shoot dry weight of plants. These findings imply that the inoculation with auxin producing fluorescent Pseudomonads could promote growth of canola. 
Keywords
Plant Growth Promoting Rhizobacteria (PGPR); Fluorescent Pseudomonas; Auxin; Canola
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