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Biabani, A. (2008). The Study of Nitrogen-Fixing Activity on Wheat Roots with Different Treatment Condition (Inoculation with Nodulation Agents and Diazotrophic Bacteria). , 22(1), 97-102. doi: 10.22092/ijsr.2008.126989
A. Biabani. "The Study of Nitrogen-Fixing Activity on Wheat Roots with Different Treatment Condition (Inoculation with Nodulation Agents and Diazotrophic Bacteria)". , 22, 1, 2008, 97-102. doi: 10.22092/ijsr.2008.126989
Biabani, A. (2008). 'The Study of Nitrogen-Fixing Activity on Wheat Roots with Different Treatment Condition (Inoculation with Nodulation Agents and Diazotrophic Bacteria)', , 22(1), pp. 97-102. doi: 10.22092/ijsr.2008.126989
Biabani, A. The Study of Nitrogen-Fixing Activity on Wheat Roots with Different Treatment Condition (Inoculation with Nodulation Agents and Diazotrophic Bacteria). , 2008; 22(1): 97-102. doi: 10.22092/ijsr.2008.126989

The Study of Nitrogen-Fixing Activity on Wheat Roots with Different Treatment Condition (Inoculation with Nodulation Agents and Diazotrophic Bacteria)

پژوهش های خاک
Volume 22, Issue 1, June 2008, Pages 97-102    PDF (827.57 K)
Document Type: Research Paper
DOI: 10.22092/ijsr.2008.126989
Author
A. Biabani*
Academic Member, Gorgan University of Agricultural and Natural Resources;
Abstract
In this study, isolated strains of nitrogen fixing bacteria and pectynolytic bacteria which can able to create paranodule on wheat seedling were selected from soils. Then the best nitrogen fixing bacteria and high capable peptonize enzyme production bacteria were chosen. 2.4-D was used as Abiological agent (Aa) and the best pectolitic bacteria as Biological agent (Ba) (Bacillus polymyxa Strain 43) for study formation Para - nodule on wheat seeding and also used diazotroph bacteria for inoculation Wheat Seeding. Nitrogen-fixation Activity on wheat roots was measured in dynamics on the 8th, 10th, 14th and 22nd days of plant growth. The experiment was carried out in 9 variants as following. 1) Wheat without any treatment (control) 2) Wheat + Aa 3) Wheat + Ba 4) Wheat inoculated + mixed cultures of diazotrophic bacteria (Arthrobacter sp + Xanthomonas sp).  5) Wheat + Bacillus polymyxa Strain 42 6) Treatment 4 +Aa 7) Treatment 5 + Aa 8) Treatment 4 + Ba 9) Treatment 5 + Ba. The formation of P- nodules on wheat roots was observed in the 4-5th day of plant growth. Their diameters were about 0.5-1 mm. It was shown that the significant differences in the number of P- nodules and also the rate of nitrogen fixing activity on wheat roots depending on the treatments. The maximum nitrogen-fixing level was recorded after wheat plant inoculation with mixed culture (Arthrobacter sp + Xanthomona sp) on the 14th day of plant growth.
Keywords
P-Nodules; Inoculation; Wheat; Nodulation Agent; Pectynolytic Bacteria; Nitrogen-Fixing
References
  1. Aceeba, E.B., N.P. Babeva and B.A. Bizov. 1991. Methods of Soil Biochemistry and Microbiology. Moscow University.
  2. Al-Mallah, M.K. and E.C. Davey Cocking. 1989. Formation of nodular structures on rice seedlings by rhizobia . J. Exper. Bot. 40: 473-478.
  3. Boiko, L.I. 1989.The influence of associative N2-fixers on cereals on Ukrainia. Microorganisms-stimulators and inhibitors of plant and animal growths. Abst. conf., 3-5 October, Tashkent, P.46. (In Russian).
  4. Chebotar, V.K. 1987. The associative diazotrophs in rhizosphere of sorghum and their influence on yield. The Ph.D thesis. Leningrad. (In Russian).
  5. Chebotar, V.K. 1995. Some aspects of interactions of associative diazotrophs with non-legume plants. Abst. IX Confer. On Nitrogen Fixation.Moscow.P.72. (In Russian).
  6. Christiansen-Weniger C. 1993. Establishment and nitrogen fixation of ammonium-excreting Azospirillum in auxine induced para-nodules of maize .Abst. 6th Int. Symposium on Nitrogen Fixation with Non-legumes. Ismailia (Egypt).
  7. Glagoleva, O.B., N.U. Kovalskaya, I.I. Kireev, E. S. Lobakova, and M.M Umarov. 1997. Para nodulation of rape inoculated with nitrogen-fixing rhizosphere bacteria, Microbiology, 66(4): 455-460.
  8. Glagoleva, O.B., N.U. Kovalskaya and M.M. Umarov. 1996. Endosymbiosis formation between nitrogen-fixing bacteria Pseudomonas caryophylli and rape root cells. Endocytobiosis and Cell Res. 11 (2/3): 147-158.
  9. Glagoleva, O.B, N.U. Kovalskaya, and M.M Umarov. 1998. The Formation of endosymbiosis between the rape roots and nitrogen-fixing rhizosphere bacteria, Dokl. Akad. Nauk, 362: 283-285.
  10. Katupitiya, S. J. Millet, M. Vesk, L.Zeman, L. Zhao, C.Elmerich and R.Kennedy . 1995. A mutant of Azospirillum brasilense sp7 impared in flocculation with modified colonization and superior nitrogen fixation in association with wheat. Appl. Environ. Microbiol. 61: 1987-1995
  11. Kennedy, Iv. R., L. Pereg-Grek, C. Wood, R. Deaker K. Gilechrist and S.Katupitiya. 1997. Biological nitrogen fixation in non-leguminous field crops: Facilitating the evolution of an effective association between Azospirillum and Wheat. Plant and soil 194: 65-79.
  12. Khammas, К. M., and P. Kaiser. 1998. Diazotrophic mixed cultures of Azospirillum and Bacillus species using pectin or rice plantlets as substrates .Proc. 11th Int. Congress on Nitrogen Fixation. Paris, P. 420.
  13. Khammas, К. М., V.V. Tran and P. Kaiser. 1993. Nitrogen fixation in rice plant inoculated with pure or mixed cultures of Azospirillum species and Bacillus polymyxa .  VI Int. Symp. On Nitrogen Fixation with Non-legumes. -  Ismailia (Egypt), P. 43.
  14. Kovalskaya, .N.U., E .S. Lobakova, and M.M. Umarov. 2001. Formation of artificial nitrogen-fixing symbiosis in rape plants (Brassica napus ) in nonsterile soil. Microbiology 70: 701-708.
  15. Patika, V.P. 1995. The mode of interactions of associative nitrogen-fixing bacteria with cereals. Abst. X Confer. On Nitrogen Fixation. Moscow. P.71. (In Russian).
  16. Peter, H., A. Sneath, B. Williams and W. Baltand. 1984. Bergey's Manual of Systematic Bacteriology: Vol. 1 and 2. P. 1120-1127.
  17. Smith D.S. and A.E. Douglas. 1987. The Biology of Symbiosis. Edward Arnold Publishers London.
  18. Zlotnikov, A.K., O.B. Glagoleva and M.M Umarov. 1998. Barley yield increase after inoculation with rhizosphere nitrogen fixing consortium Klebsiella terrigena + Bacillus firmus 11th Int. Congress on Nitrogen Fixation. Netherland, P. 413.
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