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مطالعه تأثیر بازدارندگی عصاره آبی – الکلی و نانوامولسیون اسانس گیاه مرزه خوزستانی در القای لکه موضعی ویروس موزائیک خیار (CMV) روی لوبیا چشم بلبلی | ||
| مهار زیستی در گیاه‌پزشکی | ||
| دوره 9، شماره 1 - شماره پیاپی 17، شهریور 1400، صفحه 13-23 اصل مقاله (584.88 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/bcpp.2021.126358 | ||
| نویسندگان | ||
| سمیه مصطفایی1؛ فرشاد رخشنده رو* 1؛ حسن رضادوست2؛ محسن فرزانه3 | ||
| 1گروه گیاه‌پزشکی، دانشکده علوم کشاورزی و صنایع غذایی، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران | ||
| 2گروه فیتوشیمی، پژوهشکده گیاهان و مواد اولیه دارویی، دانشگاه شهید بهشتی، اوین، تهران، ایران | ||
| 3گروه کشاورزی، پژوهشکده گیاهان و مواد اولیه دارویی، دانشگاه شهید بهشتی، اوین، تهران، ایران | ||
| چکیده | ||
| ویروس‌های گیاهی از عوامل محدودکننده در کشاورزی به‌شمار می‌روند. برای کنترل ویروسها ترکیبات طبیعی و به ویژه متابولیتهای گیاهی مورد توجه قرار گرفته است. در این راستا، اثر عصاره آبی- اتانولی (50 درصد آب) و نانوامولسیون اسانس اندام هوایی گیاه مرزه خوزستانی (Satureja khuzistanica) در چهار غلظت 5/0، 1، 2 و 4 درصد (گرم در 100 میلی لیتر) و طی سه زمان مختلف شامل همزمان و دو ساعت قبل و بعد از مایه زنی برگ بوتههای گیاه با آموده ویروس (غلظت 2 میلی گرم در میلی لیتر)، جهت کاهش آلودگی ویروس موزاییک خیار در شرایط گلخانه روی میزبان لکه موضعی لوبیا چشم بلبلی (Vigna unguiculata) مورد بررسی قرار گرفت. کمترین تعداد لکهها با کاربرد عصاره آبی -اتانولی با غلظتهای 2 و 4 درصد (78 -66% کاهش بیماری) و کاربرد نانوامولسیون اسانس با غلظت 2 درصد (59% کاهش بیماری) و دو ساعت بعد از مایهزنی با آموده ویروس حاصل شد. اندازه ذره‌ای نانوامولسیون اسانس پس از ساخت برابر با 4/119 نانومتر بود که پس از 60 روز نگهداری به 8/232 نانومتر رسید که نشان از پایداری فیزیکی مناسب نانوامولسیون میباشد. براساس آنالیز کروماتوگرافی گازی همراه با طیف سنجی جرمی (GC–MS)، ترکیبات اصلی اسانس شامل کارواکرول (5/91 %)، پارا-سیمن (5/1 درصد)، بتا-کاریوفیلن (1/1درصد) و بتا-پینن (1/1 درصد) بود. ناموامولسیون کردن اسانس باعث بهبود کارایی آن در مهار ویروس موزاییک خیار روی میزبان لوبیا چشم بلبلی شد. این تحقیق اولین گزارش از اثر ضد ویروسی ترکیبات فیتوشیمیایی گیاه مرزه خوزستانی در مهار یک ویروس گیاهی میباشد. | ||
| کلیدواژه‌ها | ||
| مرزه خوزستانی؛ عصاره؛ نانوامولسیون اسانس؛ مهار ویروس | ||
| عنوان مقاله [English] | ||
| Study of the inhibitory effects of hydro–alcoholic extract and nanoemulsion essential oil of Khuzistani savory on local lesion induction by cucumber mosaic virus (CMV) on cowpea | ||
| نویسندگان [English] | ||
| Somayeh Mostafaee1؛ Farshad Rakhshandehroo1؛ Hassan Rezadoost2؛ Mohsen Farzaneh3 | ||
| 1Department of Plant Protection, College of Agriculture Sciences and Food Industries, Science and Research Branch, Islamic Azad university, Tehran, Iran | ||
| 2Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C. Evin, Tehran, Iran | ||
| 3Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C. Evin, Tehran, Iran | ||
| چکیده [English] | ||
| Plant viruses are a limiting factor in agriculture. Natural compounds, especially herbal plant metabolites, have been considered to control viruses. In this regard, the effect of aqueous–ethanolic extract (50% water) and essential oil nanoemulsion of Satureja khuzistanica were investigated to reduce the infection of cucumber mosaic virus on the local lesion test plant of cowpea (Vigna unguiculata) in greenhouse conditions. The extract and essential oil nanoemulsion were applied in four concentrations of 0.5, 1, 2 and 4 percent (g/100 mL) and three different times including simultaneously, two hours before, and after inoculation of plant leaves with virus inoculum (concentration 2 mg/ml). The lowest number of local lesions was obtained by using 2 and 4% of hydro–ethanolic extract (by 66–78% disease reduction) and nanoemulsion of essential oil with a concentration of 2% (by 59% disease reduction), and two hours after inoculation with virus inoculum. The particle size of the nanoemulsion after preparation was 119.4 nm, which reached 232.8 nm after 60 days of storage, which indicates the good physical stability of the nanoemulsion. According to GC–MS analysis, the main constituents of essential oil were carvacrol (91.5%), para–cymene (1.5%), beta–caryophyllene (1.1%) and beta–pinene (1.1%). Nanoemulsion of this essential oil improved its efficiency to inhibit the cucumber mosaic virus on cowpea. This study is the first report of the antiviral effect of phytochemical compounds of Khuzistani savory to inhibit a plant virus. | ||
| کلیدواژه‌ها [English] | ||
| Satureja khuzistanica, extract, nanoemulsion of essential oil, plant virus inhibition | ||
| مراجع | ||
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