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تأثیر قارچکش بنومیل و سیتوکینین بر میزان آلودگی و رشد ریزنمونههای گرهای Vaccinium arctostaphylos .L. در شرایط درون شیشهای | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 10، دوره 38، شماره 1 - شماره پیاپی 111، فروردین 1401، صفحه 150-165 اصل مقاله (843.07 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2022.356225.3070 | ||
| نویسندگان | ||
| مهران نوروزپور* 1؛ ناصر زارع2؛ رسول اصغری زکریا3؛ پریسا شیخ زاده4 | ||
| 1دانشجوی دکترای بیوتکنولوژی، گروه زراعت و اصلاح نباتات، دانشگاه محقق اردبیلی، اردبیل، ایران | ||
| 2دانشیار، گروه زراعت و اصلاح نباتات، دانشگاه محقق اردبیلی، اردبیل، ایران | ||
| 3استاد، گروه زراعت و اصلاح نباتات، دانشگاه محقق اردبیلی، اردبیل، ایران | ||
| 4استادیار، گروه زراعت و اصلاح نباتات، دانشگاه محقق اردبیلی، اردبیل، ایران | ||
| چکیده | ||
| آلودگی میکروبی یکی از چالشهای اصلی و از عوامل تأثیرگذار در پاسخ ریزنمونهها در کشتهای درون شیشهای است، بهویژه در گیاهان چوبی که ریزنمونهها از رویشگاه طبیعی تهیه میگردد. در تحقیق حاضر، جهت بررسی تأثیر بنومیل بر میزان آلودگی و رشد ریزنمونههای گره قرهقاط (Vaccinium arctostaphylos L.)، از محیط کشت MS حاوی غلظتهای مختلف بنومیل (0 تا2000 میلیگرم بر لیتر) استفاده شد. همچنین، جهت بررسی برهمکنش بنومیل، محیط کشت پایه و ترکیب هورمونی بر پاسخ رشدی ریزنمونهها، محیط کشتهای پایه مختلف (MS، AN و WPM) حاوی IBA (0.1 میلیگرم بر لیتر)، سیتوکینین (زآتین، BAP و TDZ در غلظتهای 0، 0.5، 1 و 2 میلیگرم بر لیتر) و بنومیل (صفر و 150 میلیگرم بر لیتر) بررسی شدند. نتایج نشان داد که استفاده از غلظتهای مختلف بنومیل در محیط کشت پایه MS تأثیر معنیداری بر میزان آلودگی ریزنمونهها نداشت، ولی استفاده از بنومیل در غلظتهای پایینتر (400 میلیگرم بر لیتر یا کمتر)، شرایط رشدی مناسبی را فراهم کرد. اثرمتقابل تیمارها تأثیر معنیداری بر درصد زندهمانی و برگدهی ریزنمونهها داشت. در محیط MS، درصد زندهمانی و برگدهی در حضور بنومیل بیشتر از محیط فاقد بنومیل بوده، ولی در محیطهای AN و WPM درصد زندهمانی و برگدهی بهطور معنیداری در حضور بنومیل کاهش پیدا کرد. علاوهبراین، در محیطهای AN و WPM حاوی سیتوکینین، حضور بنومیل باعث کاهش معنیدار درصد برگدهی شد، ولی در محیط MS بسته به نوع و غلظت سیتوکینین تأثیر بنومیل متفاوت بود. | ||
| کلیدواژهها | ||
| بنومیل؛ زآتین؛ کشت بافت گیاهی؛ BAP؛ TDZ | ||
| عنوان مقاله [English] | ||
| Effects of benomyl and cytokinin on in vitro contamination and growth of Vaccinium arctostaphylos L. node explants | ||
| نویسندگان [English] | ||
| m. Noruzpuor1؛ n. Zare2؛ R. Asghari Zakaria3؛ P. Sheikhzade4 | ||
| 1Ph.D. student, of Agricultural Biotechnology, Department of Agronomy and Plant Breeding, University of Mohaghegh Ardabili, Ardabil, Iran | ||
| 2Department of Agronomy and Plant Breeding, University of Mohaghegh Ardabili, Ardabil, Iran | ||
| 3Department of Agronomy and Plant Breeding, University of Mohaghegh Ardabili, Ardabil, Iran | ||
| 4Department of Agronomy and Plant Breeding, University of Mohaghegh Ardabili, Ardabil, Iran | ||
| چکیده [English] | ||
| Microbial contamination is one of the main challenges and influential factors in the response of explants under in vitro cultures, especially in woody plants where the explants are prepared from natural habitats. In the present study, to investigate the effects of benomyl on microbial contamination and growth of Vaccinium arctostaphylos L. node explants, MS containing different concentrations of benomyl (0-2000 mg.L-1) was used. Furthermore, to investigate the interaction of benomyl, basal medium, and hormonal composition on the growth response of explants, different basal media (MS, AN, and WPM) containing IBA (0.1 mg.L-1), cytokinin (Zeatin, BAP, and TDZ, each at concentrations of 0, 0.5, 1, and 2 mg.L-1), and benomyl (0 and 150 mg.L-1) were studied. The results showed that benomyl in MS medium had no significant effect on the explants contamination, but use of lower benomyl concentrations (400 mg.L-1 or less) could provide better growth conditions. Interaction of treatments significantly affected the percentage of survival and explants foliation. MS contained benomyl showed higher percentage of survival and foliation than MS without benomyl, but in AN and WPM media, the percentage of survival and foliation were significantly decreased in the presence of benomyl. Furthermore, in AN and WPM media contained cytokinin, the presence of benomyl significantly reduced the foliation percentage, while in MS medium, the effect of benomyl was different depending on the concentration and cytokinin type. | ||
| کلیدواژهها [English] | ||
| Benomyl, zeatin, plant tissue culture, BAP, TDZ | ||
| مراجع | ||
|
- Eed, A.M., Reddy, S.A., Reddy, K.M., da Silva, J.A.T., Reddy, P.V., Beghum, H. and Venkatsubbaiah, P.Y., 2010. Effect of antibiotics and fungicides on the in vitro production Citrus limonia osbeck nodal segment and shoot tip explants. The Asian and Australasian Journal of Plant Science and Biotechnology, 4(1): 66-70. - Akhondzadeh, Sh., 2000. Encyclopedia of Medicinal Plants Iran. Arjmandi Publishing, 222p. - Altan, F., Bürün, B. and Shahin, N., 2010. Fungal contaminants observed during micropropagation of Lilium candidum L. and the effect of chemotherapeutic substances applied after sterilization. African Journal of Biotechnology, - Anderson, W.C., 1980. Tissue culture propagation of red and black raspberries, Rubus idaeus and R. occidentalis. Acta Horticulturae, 112: 124-132. - Bhawana, J.M., Stubblefield, A.L. and Newsome, A.B., 2015. Surface decontamination of plant tissue explants with chlorine dioxide gas. In Vitro Cellular & Developmental Biology Plant, 51: 214-219. - Brown, D.M., Groom, C.I., Cvitanik, M., Brown, B., Cooper, J. and Arditti, J., 1982. Effects of fungicides and bactericides on orchid seed germination and shoot tip cultures in vitro. Plant Cell, Tissue and Organ Culture, 1: 165-180. - Cuce, M., Bektas, E. and Sokmen, A., 2013. Micropropagation of Vaccinium arctostaphylos L. Via Lateral-bud culture. Turkish Journal of Agriculture and Forestry, 37: 40-44. - Curvetto, N., Marinangeli, P. and Mockel, G., 2006. Hydrogen peroxide in micropropagation of Lilium. A comparison with a traditional methodology. Biocell, 30(3): 497-500. - Daryani, P., Zare, N., Chamani, E., Sheikhzadeh-Mossadeg, P. and Javadi-Mojaddad, D., 2016. Eevaluation of the effects of different basal medium and plant growth regulators on in vitro growth of hazelnut. Journal of Horticultural Science, 30: - Darroudi, M., Vaezi Kakhki, M.R. and Safipour Afshar, A., 2016. Effect of type of hormone and base concentration on germination and infusion of Rosa Sanaz (Rosa chinensis var. Elvis). Iranian Journal of Plant Production Research, 23: 59-74. - Duan, Y., Zhao, F., Li, H., Zhou, Y., Zhu, X., Li, F., Chen, W. and Xue, J., 2016. Evaluation of aqueous chlorine dioxide for disinfecting plant explants. In Vitro Cellular & Developmental Biology Plant, 52: 38-44. - Firoozi, B., Nasser, Z., Sofalian, O. and Sheikhzadeh-Mosadegh, P., 2019. In vitro indirect somatic embryogenesis and secondary metabolites production in the saffron: Emphasis on ultrasound and plant growth regulators. Journal of Agricultural Sciences, 25(1): 1-10. - George, E.F., Micheal, A.H. and Greet-Jan, D.K., 2008. The components of plant tissue culture media I, macro and micro nutrients: 65-113. In: George, E.F., Micheal, A.H. and Greet-Jan, D.K., (Eds.). Plant Propagation by Tissue Culture (Vol. 1) The Background. Springer, Dordrecht, 504p. - Gajdosova, A., Ostrolucka, M.G., Libiakova, G., Ondruskova, E. and Simala, D., 2006. Microconal propagation of Vaccinium sp and Rubus sp and detection of genetic variability in culture in vitro. Juornal Fruit Ornam Plant Research, 14: 103-119. - Haldeman, J.H., Thomas, R.L. and McKamy, D.L., 1987. Use of benomyl and rifampicin for in vitro shoot tip culture of Camellia sinesis and C. japonica. HortScience, 22: 306-307. - Kazeroonian, R., Kalatejari, S., Mousavi, A. and Tohidfar, M., 2017. Reaction of various explants of a Chrysanthemum morifolium cultivar to plant growth regulators in vitro. Iranian Journal of Horticultural Science, 48(3): 527-534. - Leifert, C., Waites, W.M. and Nicholas, J.R., 1989. Bacterial contaminants of micropropagated plant cultures. Juornal of Applied Microbiology, 67: 353-361. - Linley, E., Denyer, S.P., McDonnell, G., Simons, C. and Maillard, J.Y., 2012. Use of hydrogen peroxide as a biocide, new consideration of its mechanisms of biocidal action. Journal of Antimicrobial Chemotherapy, 67: 1589-1596. - Lloyd, G. and McCown, B., 1980. Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia by use of shoot-tip culture. Combined Proceedings. Society USA, International Plant Propagators, 30: 421-427. - Meiners, J., Schwab, M. and Szankowski, I., 2007. Efficient in vitro regeneration systems for Vaccinium species. Plant Cell Tissue and Organ Culture, 89: 169-176. - Mitchell, S.A., Asemota, H.N. and Ahmad, M.H., 1995. Effects of explant source, culture medium strength, and growth regulators on the in vitro propagation of three Jamaican yams (Dioscorea cayenensis, D. trifida and D. rotundata). Journal of the Science of Food and Agriculture, 67(2): 173-180. - Murashige, T. and Skoog, F., 1977. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497. - Noruzpour, M., Zare, N., Asghari-Zakaria, R. and Sheikhzade-Mosadegh, P., 2019a. Effect of culture media and plant growth regulators on in vitro growth and production of secondary metabolites in Vaccinium arctostaphylos L. Iranian Journal of Horticultural Science, 50: 451-464. - Noruzpour, M., Zare, N., Asghari-Zakaria, R. and Sheikhzade-Mosadegh, P., 2019b. The effect of auxin and signaling compounds on growth and production of secondary metabolites in in vitro cultures of Whortleberry (Vaccinium arctostaphylos L.). Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 27: 45-58. - Ostrolucka, M.G., Libiakova, G., Ondruskova, E. and Gajdosova, A., 2004. In vitro propagation of Vaccinium species. Acta Universitias Latviensis, 607: 207-212. - Paul, A.L., Ferl, R.J., Semer, C. and Kucharek, T., 2001. The fungicidal and phytotoxic properties of benomyl and PPM in supplemented agar media supporting transgenic arabidopsis plants for a Space Shuttle flight experiment. Applied Microbiology and Biotechnology, 55: 480-485. - Reed, B.M. and Abdelnour-Esquivel, A., 1991. The use of zeatin to initiate in vitro cultures of Vaccinium species and cultivars. Horticultural Science, 26: 1320-1322. - Santos, E.P., de Souza, R.A., Medeiros, M., Alves, G.D. and Houllou, L.M., 2016. Effects of the bendazol fungicide on in vitro development of the neem (Azadirachta indica A. JUSS). Research in Biotechnology, 7: 79-85. - Sherif, F., El-Sharabasy, Z. and Zayed, E., 2018. Silver nanoparticles, antibiotics and fungicide to control microbial activity during establishment of date palm explants in vitro. Scientia Agriculturae, 21(2): 57-63. - Zare, N., Farjaminezhad, R., Asghari-Zakaria, R. and Farjaminezhad, M., 2014. Enhanced thebaine production in Papaver bracteatum cell suspension culture by combination of elicitation and precursor feeding. Natural Product Research, 28: 711-717. - Zhao, Z., Andersen, S., Ljung, K., Dolezal, K., Miotk, A., Schultheiss, S.J. and Lohmann, J.U., 2010. Hormonal control of the shoot stem-cell niche. Nature, 465: 1089-1092. | ||
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