اخبار و اعلانات

 آمار

تعداد نشریات284
تعداد نشریات سازمان82
تعداد شماره‌ها3,081
تعداد مقالات34,371
تعداد مشاهده مقاله47,880,085
تعداد دریافت فایل اصل مقاله78,877,774
Shakir, Z. M, Alhatami, A. O, Ismail Khudhair, Y, Muhsen Abdulwahab, H. (1400). Antibiotic Resistance Profile and Multiple Antibiotic Resistance Index of Campylobacter Species Isolated from Poultry. سامانه مدیریت نشریات علمی, 76(6), 1677-1686. doi: 10.22092/ari.2021.356400.1837
Z. M Shakir; A. O Alhatami; Y Ismail Khudhair; H Muhsen Abdulwahab. "Antibiotic Resistance Profile and Multiple Antibiotic Resistance Index of Campylobacter Species Isolated from Poultry". سامانه مدیریت نشریات علمی, 76, 6, 1400, 1677-1686. doi: 10.22092/ari.2021.356400.1837
Shakir, Z. M, Alhatami, A. O, Ismail Khudhair, Y, Muhsen Abdulwahab, H. (1400). 'Antibiotic Resistance Profile and Multiple Antibiotic Resistance Index of Campylobacter Species Isolated from Poultry', سامانه مدیریت نشریات علمی, 76(6), pp. 1677-1686. doi: 10.22092/ari.2021.356400.1837
Shakir, Z. M, Alhatami, A. O, Ismail Khudhair, Y, Muhsen Abdulwahab, H. Antibiotic Resistance Profile and Multiple Antibiotic Resistance Index of Campylobacter Species Isolated from Poultry. سامانه مدیریت نشریات علمی, 1400; 76(6): 1677-1686. doi: 10.22092/ari.2021.356400.1837

Antibiotic Resistance Profile and Multiple Antibiotic Resistance Index of Campylobacter Species Isolated from Poultry

Archives of Razi Institute
مقاله 13، دوره 76، شماره 6، بهمن و اسفند 2021، صفحه 1677-1686    اصل مقاله (420.82 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.356400.1837
نویسندگان
Z. M Shakir* 1؛ A. O Alhatami2؛ Y Ismail Khudhair3؛ H Muhsen Abdulwahab4
1Heath Department, National Center for Occupational Health and Safety, AL-Najaf Governorate, Iraq
2Department of Public Health, Faculty of Veterinary Medicine, University of Kufa, Kufa, Iraq
3Department of Internal and Preventive Medicine, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah, Iraq
4Department of Pathology and Poultry Diseases, Faculty of Veterinary Medicine, University of Kufa, Kufa, Iraq
چکیده
Campylobacter is a major public health problem, leading to foodborne diarrhea in the world. The current study aimed to isolate Campylobacter in different sources of poultry and determine antimicrobial susceptibility. A total of 150 fecal and 29 cloacal swabs were obtained from poultry farms (84 cloacal swabs) and live bird markets (LBMs), respectively, and 37 cecal swabs were also acquired from a local slaughterhouse located in the middle Euphrates region. Campylobacter Species (spp.) was first isolated and characterized by conventional bacteriological methods. Secondly, the antimicrobial susceptibility of isolates was investigated by disc diffusion method. The overall prevalence of Campylobacter spp. isolated from fecal cloacal and cecal poultry samples was 24% (36 out of 150). All strains were resistant to Nalidixic acid and Ciprofloxacin (100%), with high resistance to Tetracycline (88.8%), Ampicillin (83.3%), Sulpha/Trimethoprim (80.5%), Erythromycin (50%), and Ceftriaxone (50%), but less resistant to Gentamicin (30.5%), Amoxi-Clav (27.7%), and Chloramphenicol (22.2%). The majority of isolates (97.2%) scored a multiple antibiotic resistance (MAR) index of 0.3 or more, and 35 (97.2%) isolates were resistant to three or more antibiotic classes. Particularly, 61.1% of the isolates were multidrug resistance (MDR), 36.1% of the isolates were extensively drug resistant, and 2.8% of the isolates were Pan drug resistant. Moreover, the current study detected 24 multiple resistance patterns from 36 isolates of Campylobacter spp., and most of the isolates (27 out of 36) displayed an important route of resistance to Nalidixic acid, Ciprofloxacin, and Tetracycline. Based on the results, increased resistance rates to commonly used antibiotics in Campylobacter were recovered from poultry farms, LBMs, and local slaughterhouses. The majority of strains were MDR to commonly used antimicrobials with elevated MAR indices, requiring implementation of a national strategy to improve husbandry practice and the effective use of antibacterial agents, alternatives, and vaccines.
کلیدواژه‌ها
Antimicrobial resistance؛ Campylobacter؛ LBMs؛ MAR index؛ Poultry farms؛ Slaughterhouse
سایر فایل های مرتبط با مقاله
مراجع
  1. Masila NM, Ross KE, Gardner MG, Whiley H. Zoonotic and Public Health Implications of Campylobacter Species and Squamates (Lizards, Snakes and Amphisbaenians). Pathogens. 2020;9(10):799.
  2. El-Wadawe NR, Omran EA, Hazzah WA, Bakr WM. Detection of Campylobacter in Chicken Parts by Conventional Methods and Polymerase Chain Reaction with Identification of Antibiotic Resistance Profile. J High Inst Public Health. 2019;49(2):73-81.
  3. Ventola CL. The antibiotic resistance crisis: part 1: causes and threats. Pharm Ther. 2015;40(4):277.
  4. Igwaran A, Okoh AI. Human campylobacteriosis: A public health concern of global importance. Heliyon. 2019;5(11):02814.
  5. WHO. Campylobacter. 2020 Available from: https://www.who.int/news-room/fact-sheets/detail/campylobacter.
  6. Markey B, Leonard F, Archambault M, Cullinane A, Maguire D. Clinical veterinary microbiology e-book: Elsevier Health Sciences; 2013.
  7. Baali M, Lounis M, Al Amir HL, Ayachi A, Hakem A, Kassah-Laouar A. Prevalence, seasonality, and antimicrobial resistance of thermotolerant Campylobacter isolated from broiler farms and slaughterhouses in East Algeria. Vet World. 2020;13(6):1221.
  8. Gblossi Bernadette G, Eric Essoh A, Solange E, Natalie G, Souleymane B, Lamine Sébastien N, et al. Prevalence and antimicrobial resistance of thermophilic Campylobacter isolated from chicken in Côte d’Ivoire. Int J Microbiol. 2012;2012.
  9. Kanaan MHG, Abdulwahid MT. Prevalence Rate, Antibiotic Resistance and Biotyping of Thermotolerant Campylobacter Isolated from Poultry Products Vended in Wasit Markets. Curr Res Nutr Food Sci. 2019;7(3):905-17.
  10. Magiorakos A-P, Srinivasan A, Carey R, Carmeli Y, Falagas M, Giske C, et al. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect. 2012;18(3):268-81.
  11. Karikari AB, Obiri-Danso K, Frimpong EH, Krogfelt KA. Multidrug resistant Campylobacter in faecal and carcasses of commercially produced poultry. Afr J Microbiol Res. 2017;11(7):271-7.
  12. Thomas KM, de Glanville WA, Barker GC, Benschop J, Buza JJ, Cleaveland S, et al. Prevalence of Campylobacter and Salmonella in African food animals and meat: A systematic review and meta-analysis. Int J Food Microbiol. 2020;315:108382.
  13. Hazards EPoB. Scientific opinion on quantification of the risk posed by broiler meat to human campylobacteriosis in the EU. EFSA J. 2010;8(1):1437.
  14. Ibrahim JN, Eghnatios E, El Roz A, Fardoun T, Ghssein G. Prevalence, antimicrobial resistance and risk factors for campylobacteriosis in Lebanon. J Infect Dev Ctries. 2019;13(01):11-20.
  15. Wieczorek K, Osek J. Antimicrobial resistance mechanisms among Campylobacter. BioMed Res Int. 2013;2013.
  16. WHO I. Global priority list of antibiotic-resistant bacteria to guide research, discovery, and development of new antibiotics. WHO. 2017;7.
  17. Bonomo RA, Hooper DC, Kaye KS, Johnson JR, Clancy CJ, Thaden JT, et al. Gram-negative bacterial infections: research priorities, accomplishments, and future directions of the Antibacterial Resistance Leadership Group. Clin Infect Dis. 2017;64(1):30-5.
  18. Kanaan M, Khashan HT. Prevalence of multidrug resistant thermotolerant species of Campylobacter in Retail Frozen Chicken meat in Baghdad Province. Curr Res Microbiol Biotechnol. 2018;6(1):1431-40.
  19. Messad S, Hamdi T-M, Bouhamed R, Ramdani-Bouguessa N, Tazir M. Frequency of contamination and antimicrobial resistance of thermotolerant Campylobacter isolated from some broiler farms and slaughterhouses in the region of Algiers. Food Control. 2014;40:324-8.
  20. Aksomaitiene J, Ramonaite S, Tamuleviciene E, Novoslavskij A, Alter T, Malakauskas M. Overlap of antibiotic resistant Campylobacter jejuni MLST genotypes isolated from humans, broiler products, dairy cattle and wild birds in Lithuania. Front Microbiol. 2019;10:1377.
  21. Varga C, Guerin MT, Brash ML, Slavic D, Boerlin P, Susta L. Antimicrobial resistance in Campylobacter jejuni and Campylobacter coli isolated from small poultry flocks in Ontario, Canada: A two-year surveillance study. PLoS One. 2019;14(8):e0221429.
  22. Gharbi M, Béjaoui A, Ben Hamda C, Jouini A, Ghedira K, Zrelli C, et al. Prevalence and antibiotic resistance patterns of Campylobacter spp. isolated from broiler chickens in the North of Tunisia. BioMed Res Int. 2018;2018.
  23. Organization WH. Tackling antibiotic resistance from a food safety perspective in Europe: World Health Organization. REU; 2011.
  24. Kouglenou SD, Agbankpe AJ, Dougnon V, Djeuda AD, Deguenon E, Hidjo M, et al. Prevalence and susceptibility to antibiotics from Campylobacter jejuni and Campylobacter coli isolated from chicken meat in southern Benin, West Africa. BMC Res Notes. 2020;13(1):1-6.
  25. Proietti PC, Guelfi G, Bellucci S, De Luca S, Di Gregorio S, Pieramati C, et al. Beta-lactam resistance in Campylobacter coli and Campylobacter jejuni chicken isolates and the association between blaOXA-61 gene expression and the action of β-lactamase inhibitors. Vet Microbiol. 2020;241:108553.
  26. Aksomaitiene J, Novoslavskij A, Kudirkiene E, Gabinaitiene A, Malakauskas M. Whole Genome Sequence-Based Prediction of Resistance Determinants in High-Level Multidrug-Resistant Campylobacter jejuni Isolates in Lithuania. Microorganisms. 2021;9(1):66.
  27. Ghoneim NH, Sabry MA, Ahmed ZS, Elshafiee EA. Campylobacter Species Isolated from Chickens in Egypt: Molecular Epidemiology and Antimicrobial Resistance. Pak J Zool. 2020;52(3):917.
  28. Gharbi M, Béjaoui A, Hamda CB, Ghedira K, Ghram A, Maaroufi A. Distribution of virulence and antibiotic resistance genes in Campylobacter jejuni and Campylobacter coli isolated from broiler chickens in Tunisia. J Microbiol Immunol Infect. 2021.
  29. Rashid S, Azeem M, Khan SA, Shah MM, Ahmad R. Characterization and synergistic antibacterial potential of green synthesized silver nanoparticles using aqueous root extracts of important medicinal plants of Pakistan. Colloids Surf B. 2019;179:317-25.
  30. Mattheus W, Botteldoorn N, Heylen K, Pochet B, Dierick K. Trend analysis of antimicrobial resistance in Campylobacter jejuni and Campylobacter coli isolated from Belgian pork and poultry meat products using surveillance data of 2004–2009. Foodborne Pathog Dis. 2012;9(5):465-72.
  31. Hull DM, Harrell E, van Vliet AH, Correa M, Thakur S. Antimicrobial resistance and interspecies gene transfer in Campylobacter coli and Campylobacter jejuni isolated from food animals, poultry processing, and retail meat in North Carolina, 2018–2019. PloS One. 2021;16(2):e0246571.
  32. Lazo-Láscarez S, Zumbado Gutiérrez L, Duarte-Martínez F, Romero Zúñiga JJ, Arias Echandi ML, Muñoz-Vargas L. Antimicrobial resistance and genetic diversity of Campylobacter spp. isolated from broiler chicken at three levels of the poultry production chain in Costa Rica. Antimicrobial resistance of Campylobacter isolated from poultry chain. J Food Prot. 2021.
  33. Tripathi P, Banerjee G, Saxena S, Gupta MK, Ramteke P. Antibiotic resistance pattern of Pseudomonas aeruginosa isolated from patients of lower respiratory tract infection. Afr J Microbiol Res. 2011;5(19):2955-9.
  34. Paravisi M, Laviniki V, Bassani J, Kunert H, Carvalho D, Wilsmann DE, et al. Antimicrobial Resistance in Campylobacter jejuni Isolated from Brazilian Poultry Slaughterhouses. Braz J Poult Sci. 2020;22.
  35. Zhang X, Tang M, Zhou Q, Zhang J, Yang X, Gao Y. Prevalence and characteristics of Campylobacter throughout the slaughter process of different broiler batches. Front Microbiol. 2018;9:2092.
  36. Suman Kumar M, Ramees T, Dhanze H, Gupta S, Dubal Z, Kumar A. Occurrence and antimicrobial resistance of Campylobacter isolates from broiler chicken and slaughter house environment in India. Anim Biotechnol. 2021:1-9.
  1. Wei B, Cha S-Y, Yoon R-H, Kang M, Roh J-H, Seo H-S, et al. Prevalence and antimicrobial resistance of Campylobacter spp. isolated from retail chicken and duck meat in South Korea. Food Control. 2016;62:63-8.
  2. El-Naenaeey E-s, Abd El-Hamid M, Khalifa E. Prevalence and Antibiotic Resistanc Patterns of Campylobacter Species Isolated from Different Sources in Eygpt. J Microbiol Biotechnol 2021;10(6):3723.
  3. Mansour AO, Al-Jbouri SA, Mansour H. Antimicrobial resistance pattern and RAPD profile of Salmonella Ohio isolated from broiler farms in Al-Najaf and Al-Muthana provinces. Kufa J Vet Med Scien. 2013;4(2):18-30.
آمار
تعداد مشاهده مقاله: 15,667
تعداد دریافت فایل اصل مقاله: 56,307


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب