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بررسی اثر افزودن آنتیاکسیدان هایپوتائورین بر کاهش میزان پراکسیداسیون لیپید و بهبود فراسنجههای حرکتی اسپرم خروس طی انجماد و یخ گشایی | ||
| علوم دامی | ||
| دوره 34، شماره 131، شهریور 1400، صفحه 79-88 اصل مقاله (1.88 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/asj.2020.351239.2086 | ||
| نویسندگان | ||
| جمیله امامی؛ حسین دقیق کیا* ؛ غلامعلی مقدم؛ بابک قاسمی پناهی | ||
| گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز | ||
| چکیده | ||
| هدف از مطالعه حاضر بررسی اثر آنتیاکسیدان هایپوتائورین در سطوح 25/0، 5/0، 75/0 و 1 میلیمولار در رقیقکننده لیک بر پایه لسیتین طی فرایند انجماد و یخگشایی بر روی فراسنجههای حرکتی، درصد زندهمانی، یکپارچگی غشای پلاسمایی، میزان مالوندیآلدهید موجود در مایعمنی و مورفولوژی اسپرمها بود. بدین منظور نمونههای منی از 15 خروس به روش مالش پشتی-شکمی بافاصلهی دو بار در هفته جمعآوری شدند. پس از رقیقسازی و افزودن سطوح مختلفی از آنتیاکسیدانها، نمونهها در بالای بخار ازت منجمد شدند. پس از یخگشایی، نمونهها ازنظر پارامترهای عملکردی اسپرم مورد ارزیابی قرار گرفتند. نتایج آزمایش نشان داد که افزودن سطح 75/0 میلیمولار آنتیاکسیدان هایپوتائورین باعث افزایش معنی دار میزان تحرک پیشرونده، درصد تحرک کل، فراسنجه VCL، افزایش درصد زندهمانی و افزایش یکپارچگی غشای پلاسمایی نمونهها نسبت به گروه کنترل شد و همچنین سطح 75/0 میلیمولارسبب کاهش معنیدار میزان مالوندیالدهید موجود در مایع منی شد و بالاترین میزان ازنظر این پارامترها در این سطح به دست آمد (P<0.05). نتایج حاصل نشان داد که افزودن سطح بهینه از هایپوتائورین میتواند نقش محافظتی در برابر پراکسیداسیون لیپید داشته باشد و سبب بهبود فراسنجههای اسپرم خروس هنگام انجماد و یخگشایی شود و آسیبهای ناشی از تنش اکسیداتیو را کاهش دهد | ||
| کلیدواژهها | ||
| کلمات کلیدی: تنشاکسیداتیو؛ هایپوتائورین؛ گونههای فعال اکسیژن | ||
| عنوان مقاله [English] | ||
| The effect of adding hypotaurine antioxidant on reduction of lipid peroxidation and improving motility of Rooster sperm during freezing and thawing | ||
| نویسندگان [English] | ||
| jamileh emami؛ Hossein Daghigh Kia؛ Gholamali Moghaddam؛ babak gasemi panahi | ||
| university of tabriz faculty of agriculture department of animal science | ||
| چکیده [English] | ||
| The effect of adding hypotaurine antioxidant on reduction of lipid peroxidation and improving motility of Rooster sperm during freezing and thawing The aim of the present study was to investigate the antioxidant effect of hypotaurine at levels of 0.25, 0.5, 0.75 and 1 mM in lecithin-based Lake extender during the freezing and thawing process on motility, survival, plasma membrane integrity, the amount of MDA in the semen and sperm morphology parameters. For this purpose, semen samples were collected from 15 roosters by by abdominal massage method twice a week. After diluting and addation of different levels of antioxidants, the samples were frozen on liquid nitrogen vapor. After thawing, the samples were evaluated for sperm performance parameters. Results indicated an increase in sperm velocity parameters, total motility, progressive motility, viability, membrane integrity by adding 0.75 mM/L hypotaurine antioxidant compared with the other groups (P<0.05). Also, the level of 0.75 mM was significantly reduced (P <0.05) in the amount of MDA in the semen and the highest level was obtained in terms of these parameters at this level. The results showed that adding the optimal level of hypotaurine could play a protective role against lipid peroxidation and improve the appearance of rooster sperm during freezing and thawing and reduce the damage caused by oxidative stress. | ||
| کلیدواژهها [English] | ||
| Key word: Oxidative Stress, Hypotaurine, Reactive Oxygen Species | ||
| مراجع | ||
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Aksoy, N., Dogan, Y., Iriadam, M., Bitiren, M., Uzer, E., Ozgonul, A., Aksoy, S. (2012). Protective and therapeutic effects of licorice in rats with acute tubular necrosis. Journal of Renal Nutrition. 22 (3): 336-343. Alvarez, J.G., Storey, B.T. (1983). Taurine, hypotaurine, epinephrine and albumin inhibit lipid peroxidation in rabbit spermatozoa and protect against loss of motility. Biology of Reproduction. 29 (3): 548-555. Aruoma, O., Halliwell, B., Hoey, B.M., Butler, J. (1988). The antioxidant action of taurine, hypotaurine and their metabolic precursors. Biochemical Journal. 256 (1): 251-255. Badr, M., Azab, A., Rawash, Z. (2014). Effect of trehalose, cysteine and hypotaurine on buffalo bull sperm freezability, ultrastructure changes and fertilizing potentials. Assiut Vet Med J. 60 (142): 38-45. Baumber, J., Ball, B.A., GRAVANCE, C.G., Medina, V., DAVIES‐MOREL, M.C. (2000). The effect of reactive oxygen species on equine sperm motility, viability, acrosomal integrity, mitochondrial membrane potential, and membrane lipid peroxidation. Journal of andrology. 21 (6): 895-902. Bréque, C., Surai, P., Brillard, J.P. (2003). Roles of antioxidants on prolonged storage of avian spermatozoa in vivo and in vitro. Molecular Reproduction and Development: Incorporating Gamete Research. 66 (3): 314-323. Brugnon, F., Ouchchane, L., Pons-Rejraji, H., Artonne, C., Farigoule, M., Janny, L. (2013). Density gradient centrifugation prior to cryopreservation and hypotaurine supplementation improve post-thaw quality of sperm from infertile men with oligoasthenoteratozoospermia. Human reproduction. 28 (8): 2045-2057. Chatterjee, S., Gagnon, C. (2001). Production of reactive oxygen species by spermatozoa undergoing cooling, freezing, and thawing. Molecular Reproduction and Development: Incorporating Gamete Research. 59 (4): 451-458. Donnelly, E.T., McClure, N., Lewis, S.E. (2000). Glutathione and hypotaurine in vitro: effects on human sperm motility, DNA integrity and production of reactive oxygen species. Mutagenesis. 15 (1): 61-68. Fontana, M., Pecci, L., Duprè, S., Cavallini, D. (2004). Antioxidant properties of sulfinates: protective effect of hypotaurine on peroxynitrite-dependent damage. Neurochemical research. 29 (1): 111-116. Fontana, M., Amendola, D., Orsini, E., Boffi, A., Pecci, L. (2005). Oxidation of hypotaurine and cysteine sulphinic acid by peroxynitrite. Biochemical Journal. 389 (1): 233-240. Gutteridge, J.M., Halliwell, B. (2000). Free radicals and antioxidants in the year 2000: a historical look to the future. Annals of the New York Academy of Sciences. 899 (1): 136-147. HOLMES, R.P., GOODMAN, H.O., SHIHABI, Z.K., JAROW, J.P. (1992). The taurine and hypotaurine content of human semen. Journal of Andrology. 13 (3): 289-292. Kelso, K., Cerolini, S., Noble, R., Sparks, N.C., Speake, B. (1996). Lipid and antioxidant changes in semen of broiler fowl from 25 to 60 weeks of age. Reproduction. 106 (2): 201-206. Lambert, I.H., Jensen, J.V., Pedersen, P.A. (2014). mTOR ensures increased release and reduced uptake of the organic osmolyte taurine under hypoosmotic conditions in mouse fibroblasts. American Journal of Physiology-Cell Physiology. 306 (11): C1028-C1040. Martínez-Páramo, S., Diogo, P., Dinis, M., Soares, F., Sarasquete, C., Cabrita, E. (2013). Effect of two sulfur-containing amino acids, taurine and hypotaurine in European sea bass (Dicentrarchus labrax) sperm cryopreservation. Cryobiology. 66 (3): 333-338. Mehdipour, M., Daghigh Kia, H., Moghaddam, G., Hamishehkar, H. (2018). Effect of egg yolk plasma and soybean lecithin on rooster frozen-thawed sperm quality and fertility. Theriogenology. 116 89-94. Mocé, E., Vicente, J.S. (2009). Rabbit sperm cryopreservation: a review. Animal reproduction science. 110 (1-2): 1-24. Mrsny, R.J., Waxman, L., Meizel, S. (1979). Taurine maintains and stimulates motility of hamster sperm during capacitation in vitro. Journal of Experimental Zoology. 210 (1): 123-128. Pasantes-Morales, H., Cruz, C. (1985). Taurine and hypotaurine inhibit light-induced lipid peroxidation and protect rod outer segment structure. Brain research. 330 (1): 154-157. S, S., M, G., J, R., D, H., J, H., N, Z. (2017). Evaluation the effects of different levels of vitamin E and Nano Selenium on sperm quality parameters of Leghorn rooster during chilled storage on 4◦C. Journal of Animal Science Researches. 26 (4): 59-70. Safa, S., Moghaddam, G., Jozani, R.J., Daghigh Kia, H., Janmohammadi, H. (2016). Effect of vitamin E and selenium nanoparticles on post-thaw variables and oxidative status of rooster semen. Animal reproduction science. Seifi-Jamadi, A., Zhandi, M., Ansari, M. (2019). The effect of Chrysin inclusion to Beltsville extender on cooling storage of rooster sperm. Journal of Animal Research (Iranian Journal of Biology). 32 (1): 36-48. Shaikh, K., SUTHAR, H.N.B., SUTARIA, P., SHARMA, V. (2016). 13. COMPARISON OF FRESH SEMEN PARAMETERS WITH FROZEN THAWED SEMEN FOLLOWING INCORPORATION OF TREHALOSE by KQ SHAIKH, HC NAKHASHI1, BN SUTHAR2, PT SUTARIA3 AND VK SHARMA4. Life Sciences Leaflets. 76 107 to 115-107 to 115. Sinet, P.-M., Garber, P. (1981). Inactivation of the human CuZn superoxide dismutase during exposure to O2− and H2O2. Archives of Biochemistry and Biophysics. 212 (2): 411-416. Singh, V., Atreja, S., Kumar, R., Chhillar, S., Singh, A. (2012). Assessment of intracellular Ca2+, cAMP and 1, 2‐Diacylglycerol in cryopreserved buffalo (Bubalus bubalis) spermatozoa on supplementation of taurine and trehalose in the extender. Reproduction in domestic animals. 47 (4): 584-590. Tarvis, K.M., 2013. New methods for cryopreserving rooster spermatozoa, Colorado State University. Libraries. Wang, Y., Sharma, R., Agarwal, A. (1997). Effect of cryopreservation and sperm concentration on lipid peroxidation in human semen. Urology. 50 (3): 409-413. Zini, A., Garrels, K., Phang, D. (2000). Antioxidant activity in the semen of fertile and infertile men. Urology. 55 (6): 922-926. | ||
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