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Radha Abbas Hasoon, M, Jawad Kadhim, N. (1400). Improvement of the Selectivity Index (SI) and Cytotoxicity Activity of Doxorubicin Drug by Panax ginseng Plant Extract. سامانه مدیریت نشریات علمی, 76(3), 659-666. doi: 10.22092/ari.2021.355413.1681
M Radha Abbas Hasoon; N Jawad Kadhim. "Improvement of the Selectivity Index (SI) and Cytotoxicity Activity of Doxorubicin Drug by Panax ginseng Plant Extract". سامانه مدیریت نشریات علمی, 76, 3, 1400, 659-666. doi: 10.22092/ari.2021.355413.1681
Radha Abbas Hasoon, M, Jawad Kadhim, N. (1400). 'Improvement of the Selectivity Index (SI) and Cytotoxicity Activity of Doxorubicin Drug by Panax ginseng Plant Extract', سامانه مدیریت نشریات علمی, 76(3), pp. 659-666. doi: 10.22092/ari.2021.355413.1681
Radha Abbas Hasoon, M, Jawad Kadhim, N. Improvement of the Selectivity Index (SI) and Cytotoxicity Activity of Doxorubicin Drug by Panax ginseng Plant Extract. سامانه مدیریت نشریات علمی, 1400; 76(3): 659-666. doi: 10.22092/ari.2021.355413.1681

Improvement of the Selectivity Index (SI) and Cytotoxicity Activity of Doxorubicin Drug by Panax ginseng Plant Extract

Archives of Razi Institute
مقاله 28، دوره 76، شماره 3، آذر 2021، صفحه 659-666    اصل مقاله (390.26 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.355413.1681
نویسندگان
M Radha Abbas Hasoon؛ N Jawad Kadhim*
University of Kufa, Faculty of Science, Department of Biology, Iraq
چکیده
In China, Japan, and Korea, Panax ginseng has been used in traditional medicine for thousands of years. Panax is a plant used as a general tonic or adaptogen for chronically ill patients. The current study evaluated the cytotoxicity of Panax ginseng extract (PGE). Different cell lines (HCT-116, LNCaP, and normal cell line VERO) were treated with different inhibitory agentsat different concentrations (1000, 500, 250, 125, 62.5, and 31.25 µg/ml) as follows: G1 (Methanol Panax ginseng extract, PGE), G2 (Doxorubicin, DOX), and G3 (Methanol Panax ginseng extract +DOX, PDD). Each inhibitory agent group was used to treat the cancerous cell lines HCT-116, LNCaP, and normal cell line (VERO) to obtain IC50% by MTT assay. The inhibitory ability of the 1000 μg/ml PGE was significantly increased in all the three-cell lines compared with other concentrations. The recorded data revealed that the inhibition ability of PGE and Doxorubicin towards the HCT-116 cell line significantly increased compared with the other cell lines. The interaction between different PGE concentrations and cell lines showed that the 1000 μg/ml PEG had the highest inhibitory effects on HCT-116 compared with other combinations. The interaction between different DOX concentrations and different types of cell lines showed that the 1000 μg/ml DOX had the highest inhibitory effects on LNCap compared with other combinations. The PGD inhibition ability reflected a significantly higher difference toward the HCT-116 cell line as compared with other cell lines. IC50% is the concentrations (µg/ml) to kill 50% of cell line. It was calculated by MTT assay for three cell lines: HCT-116, LNCaP, and VERO. The rate of effectiveness of the inhibitory factors (PGE, DOX, and PGD) showed highly significant differences toward the cell line HCT-116 compared to the other cell lines. This indicates the safety of the PGE compound and its low toxicity toward normal cells, quite the opposite of cancer cells as compared to the common drug DOX and combined PGD (PGE+DOX). PGD combined with DOX (PGE + DOX) showed antagonistic results toward the HCT116, LNCaP, and VERO cell lines, while UDE combined with DOX (UDE+DOX) showed synergistic activity.
کلیدواژه‌ها
Panax ginseng؛ HCT-116؛ LNCap؛ VERO؛ doxorubicin؛ interaction index؛ selectivity index
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