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DNA extraction from chicken blood using a modified Salting-out method | ||
| علوم و فنون دامپروری | ||
| Volume 9, Issue 34, June 2020, Pages 3-10 PDF (1.63 M) | ||
| DOI: 10.22092/aasrj.2019.128093.1188 | ||
| Authors | ||
| Ali javanrouh* 1; Saber Jelokhani-niaraki2 | ||
| 1Animal Science Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| 2faculty member at the Biotechnology department in the Animal Science Research Institute of Iran | ||
| Abstract | ||
| Extraction of high-quality genomic DNA is of great importance in various genetic and breeding studies. Although the principles of DNA extraction from different plant, animal, and bacterial cells are relatively similar, but in order to make it more efficient, save the time and cost, there is a need to make some changes in the DNA extraction method based on the purpose of research, the species and cell kind. One of the DNA extraction methods is salting-out method, which is optimized to the livestock species by making some changes. The objective of this study was to present an optimized Salting-Out method for DNA extraction from chicken blood. For this purpose, some changes were made in the blood volume, the amount of consumed materials and buffers, and also the method of condensed DNA collection. To evaluate the optimized method, 400 chicken blood samples were used for genomic DNA extraction. Then the quality and quantity of extracted DNA were determined by electrophoresis on 1% agarose gel and spectrophotometry methods. The results of electrophoresis showed that sharp DNA bands visualized in gel with the lowest fracture rate, indicating the desired quality of extracted DNA samples. Also, the results of quality assessment of DNA samples using spectrophotometry showed that the absorption ratio of 260 to 280 nanometer in most samples range from 1.7 to 1.9, indicating that they are appropriate for use in next steps including polymerase chain reaction. | ||
| Keywords | ||
| chicken; DNA extraction; Salting-Out method; DNA Qualitative evaluation | ||
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