اخبار و اعلانات

 آمار

تعداد نشریات282
تعداد نشریات سازمان80
تعداد شماره‌ها3,188
تعداد مقالات35,336
تعداد مشاهده مقاله51,472,298
تعداد دریافت فایل اصل مقاله91,335,255
Azimi, Maryam, Ahmadi Golsefidi, Mazyar, Varasteh Moradi, Ali, Ebadi, Mehdi, Zafar Mehrabian, Ramin. (1400). Separation and Purification of Galegine using Column Chromatography followed by Molecularly Imprinted Polymer Technique. سامانه مدیریت نشریات علمی, 10(2), 149-160. doi: 10.22092/jmpb.2020.341244.1168
Maryam Azimi; Mazyar Ahmadi Golsefidi; Ali Varasteh Moradi; Mehdi Ebadi; Ramin Zafar Mehrabian. "Separation and Purification of Galegine using Column Chromatography followed by Molecularly Imprinted Polymer Technique". سامانه مدیریت نشریات علمی, 10, 2, 1400, 149-160. doi: 10.22092/jmpb.2020.341244.1168
Azimi, Maryam, Ahmadi Golsefidi, Mazyar, Varasteh Moradi, Ali, Ebadi, Mehdi, Zafar Mehrabian, Ramin. (1400). 'Separation and Purification of Galegine using Column Chromatography followed by Molecularly Imprinted Polymer Technique', سامانه مدیریت نشریات علمی, 10(2), pp. 149-160. doi: 10.22092/jmpb.2020.341244.1168
Azimi, Maryam, Ahmadi Golsefidi, Mazyar, Varasteh Moradi, Ali, Ebadi, Mehdi, Zafar Mehrabian, Ramin. Separation and Purification of Galegine using Column Chromatography followed by Molecularly Imprinted Polymer Technique. سامانه مدیریت نشریات علمی, 1400; 10(2): 149-160. doi: 10.22092/jmpb.2020.341244.1168

Separation and Purification of Galegine using Column Chromatography followed by Molecularly Imprinted Polymer Technique

Journal of Medicinal plants and By-products
دوره 10، شماره 2، آذر 2021، صفحه 149-160    اصل مقاله (733.84 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22092/jmpb.2020.341244.1168
نویسندگان
Maryam Azimi؛ Mazyar Ahmadi Golsefidi؛ Ali Varasteh Moradi* ؛ Mehdi Ebadi؛ Ramin Zafar Mehrabian
Department of Chemistry, Faculty of Sciences, Gorgan Branch, Islamic Azad University, Gorgan, Iran
چکیده
Galega officinalis L. (Papilionaceae) is a native to southeastern Europe that is used in the treatment of various diseases including diabetes. The extract of this plant contains many effective compounds such as Galegine that is a guanidine alkaloid. In this research, column chromatography with a molecularly imprinted polymer (MIP) technique was used to achieve this valuable material. The extracted components were separated by column chromatography using silica gel as stationary phase. For eluting of components from the Galegine extract, once mobile phase was completely non-polar hexane solvent, and then, the polarity of the solvent was increased until all the remaining components were removed. Finally, 11 fractions were achieved. Thin-layer chromatography (TLC) method was used to identify the isolated components. Then, the Galegine in the extraction fraction was purified using MIP technique. The results showed that the chromatographic separation method led to removing significantly the interfacial compounds. Interestingly, it was resulted that while the column chromatography was priority used from MIP adsorption, the purification and isolation efficiency of Galegine was enhanced up to 17 times compared to using MIP alone.
کلیدواژه‌ها
Extracted Galegine؛ Column Chromatography؛ Molecularly Imprinted Polymer؛ Isolation
مراجع
  1. Yaghmeai P, Gheibi S, Varzandi T, Ghasemi A. Effects of Oral Sodium Nitrate Administration on Cell Blood Count in Obese Type 2 Diabetic Male Rats. Iranian J Endocrinology Metabolism. 2018; 19:340-348.
  2. Genuth S, Eastman R, Kahn R, Klein R, Lachin J, Lebovitz H, Nathan D, Vinicor F. Implications of the United Kingdom prospective diabetes study. Diabetes care. 2003; 26: S28.
  3. Han Y, Bearse MA, Schneck ME, Barez S, Jacobsen CH, Adams AJ. Multifocal electroretinogram delays predict sites of subsequent diabetic retinopathy. Investigative Ophthalmol Visual Sci. 2004; 45:948-954.
  4. Esmaillzadeh A, Tahbaz F, Gaieni I, Alavi-Majd H, Azadbakht L. Cholesterol-lowering effect of concentrated pomegranate juice consumption in type II diabetic patients with hyperlipidemia. International J Vitamin Nutrition Res. 2006; 76:147-151.
  5. Hashem Dabaghian F, Kamalinejad M, Shojaii A, Abdollahi Fard M, Ghushegir SA. Review of antidiabetic plants in Iranian traditional medicine and their efficacy. J Med Plants. 2012; 1:1-1.
  6. Eidi A, Eidi M, Darzi R. Antidiabetic effect of Olea europaea L. in normal and diabetic rats. Phytotherapy Research: An International J Devoted to Pharmacol Toxicol Evaluation Natural Product Derivatives. 2009; 23:347-350.
  7. Shokri M, Safaian N. The study of medicinal plants in Mazandaran (northern Iran). In WOCMAP I-Medicinal and Aromatic Plants Conference: part 1 of 4 333. 1992;165-174.
  8. Coman C, Rugina OD, Socaciu C. Plants and natural compounds with antidiabetic action. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2012; 40:314-325.
  9. Atanasov AT. Effect of the water extract of G. officinalis L. on human platelet aggregation in vitro. Phytotherapy Res. 1994; 8:314-316.
  10. Mooney MH, Fogarty S, Stevenson C, Gallagher AM, Palit P, Hawley SA, Hardie DG, Coxon GD, Waigh RD, Tate RJ, Harvey AL. Mechanisms underlying the metabolic actions of galegine that contribute to weight loss in mice. British J Pharma. 2008; 153:1669-1677.
  11. KARAKAŞ FP, CİNGÖZ G, TÜRKER A. Enhancement of direct shoot regeneration and determination of bioactive secondary metabolites in leaves of G. officinalis L. Turkish J Biology. 2016; 40:1311-1319.
  12. Oubre AY, Carlson TJ, King SR, Reaven GM. From plant to patient: an ethnomedical approach to the identification of new drugs for the treatment of NIDDM. Diabetologia. 1997; 40:614-617.
  13. Palit P, Furman BL, Gray AI. Novel weight‐reducing activity of G. officinalis in mice. J Pharmacy Pharma. 1999; 51:1313-1319.
  14. Vuksan V, Sievenpiper JL. Herbal remedies in the management of diabetes: lessons learned from the study of ginseng. Nutrition Meta Cardiovascular Diseases. 2005; 15:149-160.
  15. Mandal V, Mohan Y, Hemalatha S. Microwave assisted extraction—an innovative and promising extraction tool for medicinal plant research. Pharmacog Reviews. 2007; 1:7-18.
  1. Larson EB, Wang L, Bowen JD, McCormick WC, Teri L, Crane P, Kukull W. Exercise is associated with reduced risk for incident dementia among persons 65 years of age and older. Annals Internal Med. 2006; 144:73-81.
  2. Wagner H, Bladt S. Plant drug analysis: a thin layer chromatography atlas. Springer Sci Business Media. 1996.
  3. Nethravathi C, Rajamathi M. Chemically modified graphene sheets produced by the solvothermal reduction of colloidal dispersions of graphite oxide. Carbon. 2008; 46:1994-1998.
  4. Benn MH, Shustov G, Shustova L, Majak W, Bai Y, Fairey NA. Isolation and characterization of two guanidines from galega orientalis lam. cv. gale (fodder galega). J Agric Food Chem. 1996; 44:2779-2781.
  5. Atanasov AT, Tchorbanov B. Antiplatelet aggregation activity of a fraction isolated from Galega officinalis L. J Herbs Spices Med Plants. 2003; 10:63-71.
  6. Hande PE, Samui AB, Kulkarni PS. A molecularly imprinted polymer with flash column chromatography for the selective and continuous extraction of diphenyl amine. RSC Advances. 2015; 5:73434-7343.
  7. Davoodi P, Ghoreishi SM, Hedayati A. Optimization of supercritical extraction of galegine from G. officinalis L.: Neural network modeling and experimental optimization via response surface methodology. Korean J Chem Engin. 2017; 34:854-865.
آمار
تعداد مشاهده مقاله: 1,177
تعداد دریافت فایل اصل مقاله: 1,537


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب