Motamed Rezaei, O., Mirzaei, M., Jahani, M., Ramezani, A. (2021). Isolation and molecular identification of fungal contamination of corn and soybean diets of poultry in Birjand area. , 34(4), 147-157. doi: 10.22092/vj.2020.343043.1723
O Motamed Rezaei; M. R Mirzaei; M Jahani; A. A Ramezani. "Isolation and molecular identification of fungal contamination of corn and soybean diets of poultry in Birjand area". , 34, 4, 2021, 147-157. doi: 10.22092/vj.2020.343043.1723
Motamed Rezaei, O., Mirzaei, M., Jahani, M., Ramezani, A. (2021). 'Isolation and molecular identification of fungal contamination of corn and soybean diets of poultry in Birjand area', , 34(4), pp. 147-157. doi: 10.22092/vj.2020.343043.1723
Motamed Rezaei, O., Mirzaei, M., Jahani, M., Ramezani, A. Isolation and molecular identification of fungal contamination of corn and soybean diets of poultry in Birjand area. , 2021; 34(4): 147-157. doi: 10.22092/vj.2020.343043.1723

Isolation and molecular identification of fungal contamination of corn and soybean diets of poultry in Birjand area

تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
Article 17, Volume 34, Issue 4, January 2022, Pages 147-157    PDF (777.02 K)
Document Type: Full Research Paper
DOI: 10.22092/vj.2020.343043.1723
Authors
O Motamed Rezaei1; M. R Mirzaei* 2; M Jahani3; A. A Ramezani4
1Department of Occupational Health , Birjand university of medical sciences , Birjand, Iran
2Plant Protection Research Department, South Khorasan Agricultural and Natural Resources Research and Education Center, AREEO, Birjand, Iran
3Department of Plant Protection, College of Agriculture, University of Birjand, Birjand, Iran
4Social Determinant of Health Research center ,Department of Epidemiology and Biostatistics , Birjand university of medical sciences , Birjand, Iran .
Abstract
Fungal growth on poultry feed decreases their nutritional value and constitutes health hazard. To produce healthy food for consumers, recognizing and controlling the contamination of poultry feed is necessary. A total of 212 poultry feed samples including two different feeds were collected and by using dilution plating technique, successive dilutions were prepared and each of dilutions was cultured on solid fungal media cultures. Different fungal species such as Penicillium sp., P.aurantiogriseum, P.chrysogenum, Aspergillus flavus and Fusarium proliferatum were isolated from poultry feed of Birjand area of south Khorasan province and then purified and identified based on morphological and molecular characteristics. DNA extraction were performed by modified Chelex 100 method and Polymerase chain reaction (PCR) method using amplification of  internal transcribed spacer (ITS) region used for molecular identification of fungal taxa. Rhizopus isolates identified based on morphological features. The highest and lowest fungal colonies were Penicillium(0.617±1.16) and Fusarium (0.075±0.417) receptively. Based on this study, controlling the contamination of poultry feed is recommended.
Keywords
Corn and soybean diets; fungul contamination; molecular identification
References

1. Abbas H.K., R.M. Zablotowicz, M.A. Wearver, B.W. Horn, W. Xie and W.T. Shier .2004. Comparison of cultural and analytical methods for determination of production by Mississipi Delta Aspergillus isolates. Canadian Journal of Microbiology 50: 193-199.
2. Cotty PJ. 1994. Influence of field application of an aflatoxigenic strain of Aspergillus flavus on the populations of A. flavus infecting cotton bolls and on the aflatoxin content of cottonseed. Phytopathology 84: 1270-1277.
3. Darvishnia R., M. Darvishnia and M.H. Gharouni. 2019. Investigating the toxicity of fungi isolated from livestock feed and poultry in Khorramabad county. Biological Journal of Microorganism 31: 51-69. (in Persian).
4. Deepthi B.V., A. P. Gnanaprakash and M.Y. Sreenivasa. Effect of c-irradiation on fumonisin producing Fusarium associated with animal and poultry feed mixtures. Biotech 7: 2-8. 
5. Ezekwueche S.N., C.U. Umedum, C.C. Uba and I.S. Anagor. 2018. Fungi Isolated from Poultry Droppings Express Antagonism against Clinical Bacteria Isolates. Microbiology Research Journal International 26:1-8. 
6. Fadaeifard F., M. Raiisy, H. Koorangi, E. Rahimi and R. Pirzadeh. 2013. Determination of Ochratoxin A in the rainbow trout (Oncorhynchus mykiss) feed in Chaharmahal Va Bakhtiary province by ELISA assay. Food Hygiene 2: 71-79. (in Persian).
7. Fakruddin M., A. Chowdhury, M.N. Hossain and M.M. Ahmed. 2015. Characterization of aflatoxin producing Aspergillus flavus from food and feed samples. Springerplus 4:159.
8. Frisvad J.C. and R.A. Samson. 2005. Polyphasic taxonomy of Penicillium subgenus Penicillium, a guide to identification of food and air-borne terverticillate Penicillia and their mycotoxins, Studies in Mycology. 49: 1–173.
9. Ghaemmaghami S.S., M. Modirsaneii, A.R. Khosravi and M. Razzaghi-Abyaneh M. 2016. Study on mycoflora of poultry feed ingredients and finished feed in Iran. Iranian Journal of Microbiology 8:47-54. 
10. Greco M.V., M.L. Franchi, S.L. Rico Golba, A.G. Pardo and G.N.Pose. 2014. Mycotoxins and mycotoxigenic fungi in poultry feed for food-producing animals. Scientific World Journal 2014: 1-9.
11. Greeff-Laubscher M.R., I. Beukes, G. J. Marais and K. Jacobs. 2020. Mycotoxin production by three different toxigenic fungi genera on formulated abalone feed and the effect of an aquatic environment on fumonisins. Mycology 11: 105-11.
12. Hajnal E.J., J. Kos, J. Krulj, S. Krstović, I. Jajić and L. Pezo. 2017. Aflatoxins contamination of maize in Serbia: The impact of weather conditions in 2015. Food Additives and Contaminatios, Part A, 1999-2010.  
13. Halt M. 1994. Aspergillus flavus and aflatoxin B1 in flour production. European Journal of Epidemiology 11: 555–558.
14. Hirata T. and S. Takamatsu. 1996. Nucleotide sequence diversity of rDNA internal transcribed spacers extracted from conidia and cleistothecia of several powdery mildew fungi. Mycoscience 37: 265– 270.
15. Ibrahim A.d., A. Mahmuda, A.A. Farouq, M.J. Muazu, R.M. Aliyu, I.M.  Saadat and A.A. Aliero. 2018. Effects of Replacing Maize with Rhizopus Oryzae Fermented Mangifera Indica Seed Kernels on Broilers Chicken Growth Performance. Nutrition and Food Science International Journal. 4: 103-109.  
16. Iram S., S.K. Fareed, M. Chaudhary, M.N. Igbal, R. Ghani, T.A. Khan and T. Abbas. 2019. Identification of Aspergillus flavus and aflatoxin in home mix layer poultry feed in relation to seasons in Karachi, Pakistan. Tropical Animal Health and Production 51: 1321–1327. 
17. Labuda R., D. Tancinova and K. Hudec. 2003. Identification and enumeration of Fusarium species in poultry feed mixtures from Slovakia. Annals of Agricultural and Environmental Medicine 10: 61-66. 
18. Labuda R and D. Tancinova. 2006. Fungi recovered from Slovakian poultry feed mixtures and their toxinogenity. Annals of Agricultural and Environmental Medicine 13: 193-200.
19. Leslie J.F. and B.A. Summerell. 2006. The Fusarium laboratory manual, Third Edition, Blackwell Publishing Professional, Ames.
20. Magnoli C.E. and C.A. da Rocha Rosa. 2013. Fungal contamination and determination of fumonisins and aflatoxins in commercial feeds intended for ornamental birds in Rio de Janeiro, Brazil. Letters in Applied Microbiology 57:405-411.
21. Munkvold G.P., A. Silvina, I. Taschl and  C. Gruber-Dorninger. 2019. Mycotoxins in Corn: Occurrence, Impacts, and Management In: Corn: Chemistry and Technology, Third Edition, Pages 235-287.
22. Piecková E. and Z. Jesenská. 2001. Fusarium moniliforme, Fusarium subglutinans and Aspergillus flavus in maize products in Slovakia. Czech Mycology 53: 229-235. 
23. Salehan Z., S. Eidi, M. Mohsenzadeh and M. Azizzadeh. 2018. Determination of fungal contamination of poultry feed and its ingredients in broiler farms in Torbat-Heydarieh, Khorasan Razavi province, Iran. Journal of Veterinary Research 72: 447-456.
24. Sugiharto S. 2019. A review of filamentous fungi in broiler production. Annals of Agricultural Sciences. 64: 1-8.
25. Tamura K., G. Stecher, D. Peterson, A. Filipski and  Kumar, S. 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular biology and evolution 30: 2725-2729.
26. Variane A.C.F., F.C. Santos, F.F. Castro, I.P. Barbosa-Tessmann, G.T. Santos and M.S. Santos Pozza. 2018. The occurrence of aflatoxigenic Aspergillus spp. in dairy cattle feed in Southern Brazil. Brazilian Journal of Microbiology 49 919–928.
27. Walsh P.S., DA. Metzger and R. Higuchi. 1991. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques 10: 506–13.
28. White T.J., T. Bruns, S. Lee and J.W. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editor. PCR Protocols: A Guide to Methods and Applications. New York: Academic Press Inc.
29. Wu W. 1997. Counteracting Fusarium proliferatum toxicity in broiler chicks by supplementing drinking water with Poultry Aid Plus®. Poultry Science 76: 463–468.

 

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