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برآورد هم خونی ژنومی و ارزیابی هموزیگوسیتی ایجاد شده در گاوهای سرابی و نجدی | ||
| علوم دامی | ||
| دوره 33، شماره 127، شهریور 1399، صفحه 13-30 اصل مقاله (1.74 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/asj.2019.124197.1819 | ||
| نویسندگان | ||
| احد خصالی اقطاعی1؛ مهدی وفای واله* 2؛ حسین مرادی شهر بابک3؛ غلامرضا داشاب2 | ||
| 1دانش آموخته دکتری ژنتیک و اصلاح نزاد دام، طیور، گروه علوم دامی، دانشکده کشاورزی، دانشگاه زابل، زابل | ||
| 2دانشیار، گروه علوم دامی، دانشکده کشاورزی، دانشگاه زابل، زابل | ||
| 3استادیار، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران | ||
| چکیده | ||
| مطالعه حاضر به منظور برآورد سطح همخونی ژنومی با استفاده از تجزیه (FROH) ROH در دو توده گاو بومی ایران شامل سرابی و نجدی و همچنین مقایسه برآوردهای FROH با دیگر برآوردهای ضریب همخونی بر اساس ماتریس رابطه ژنوم (FGRM)، درصد SNPs هموزیگوت (FHOM) و اطلاعات شجرهنامه (FPed) انجام شد. برای انجام این کار، به ترتیب تعداد 213 و 211 نمونه به طور تصادفی از جمعیت سرابی و نجدی انتخاب شدند. نمونهها با استفاده از ریز تراشه Illumina BeadChip 40K v2 تعیین ژنوتیپ شدند. علاوه بر این، تعداد 30 نمونه از گاوهای شیری هلشتاین ایران از مرکز اصلاح نژاد ایران، مورد بررسی قرارگرفتند. آنالیز ژنومی با استفاده از برنامههای CFC ، Excel، Plink، SNeP انجام شد. در مجموع 2030 بلوک هاپلوتیپی در جمعیت شناسایی شدند. بیشترین و کمترین تعداد قطعات (ROHs) ROH در بین جمعیت نجدی و سرابی مشاهده شد. علاوه بر این، طول ROH در بین نژادهای مختلف متفاوت بود (۰۵/۰p <). جمعیت نجدی بیشترین تعداد ROH را در دستههای با طول متفاوت Mb ) ROH8-4، Mb16-8 وMb 16<) داشتند. حداکثر ضریب همبستگی بین ضرایب همخونی برآورد شده بین FPed و FROH> 4Mb (592/0) در جمعیت سرابی بدست آمد (۰۰۱/۰p <). نتایج این مطالعه وجود همخونی، حداقل در پنج نسل اخیر جمعیت بومی مورد مطالعه را نشان میدهد و برنامههای حفاظتی باید به منظور مدیریت سطوح همخونی در هر دو جمعیت صورت گیرد. | ||
| کلیدواژهها | ||
| توده های گاو بومی؛ هم خونی ژنومی؛ هموزیگوسیتی ایجاد شده | ||
| عنوان مقاله [English] | ||
| Genomic inbreeding estimation and evaluation of runs of homozygosity in Sarabi and Najdi cattle populations. | ||
| نویسندگان [English] | ||
| Ahad Khasali aghtaei1؛ Mehdi Vafa Valleh2؛ Hossein Moradi Shahrebabak3؛ Golamreza Dashab2 | ||
| 1Agricultural Jahad Organization of Kerman Province, Kerman, Iran | ||
| 2Assistant Professor, Animal Breeding and Genetics, University of Zabol | ||
| 3assistant professor/Department of Animal Sciences, University College of Agriculture and Natural Resources, University of Tehran | ||
| چکیده [English] | ||
| The present study was carried out to estimate levels of genomic inbreeding based on ROH (FROH) analysis in the two Iranian native cattle populations including Sarabi and Najdi, as well as comparing the FROH estimates with other inbreeding estimates obtained based on the genomic relationship matrix (FGRM), percentage of homozygous SNPs (FHOM) and pedigree information (FPed). To do this, 213 and 211 samples were randomly selected from Sarabi and Najdi populations, respectively. The samples were genotyped using the Illumina BeadChip40K v2 microchip. In addition, 30 samples of Holstein dairy cattle of Iran provided by Animal Breeding Center of Iran, were included in the analysis . Genomic analysis was performed using CFC, Excel, Plink, SNeP programs. A total of 2030 haplo-blocks were identified in the populations. The highest and lowest number of ROH segments (ROHs) was observed within Najdi and Sarabi Population, respectively. Furthermore, ROH length were found to be significantly different among breeds (p < 0.05). Najdi population had the highest number of ROH across different categories of ROH length (4-8Mb, 8-16Mb and >16Mb). Maximum correlation coefficient among the estimated inbreeding coefficients was obtained between FPed and FROH>4Mb (0.592) in the Sarabi population (p < 0.001). The results of this study indicate that presence of inbreeding at least in the five early generations of the studied native populations and the conservation programs must be taken to manage the inbreeding levels in both populations. | ||
| کلیدواژهها [English] | ||
| Genomic inbreeding, Local cattle breeds, Runs of homozygosity | ||
| مراجع | ||
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آمار تعداد مشاهده مقاله: 925 تعداد دریافت فایل اصل مقاله: 593 |
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