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بررسی چندشکلی ژنهای DNMTs در گاو سیستانی با استفاده از روش PCR-SSCP | ||
| علوم دامی | ||
| دوره 33، شماره 126، خرداد 1399، صفحه 73-82 اصل مقاله (1.69 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/asj.2019.124714.1847 | ||
| نویسندگان | ||
| سجاد شهدادی ساردو1؛ مهدی وفای واله* 2؛ غلامرضا داشاب3؛ محمد رکوعی3 | ||
| 1دانش آموخته کارشناسی ارشد علوم دامی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران | ||
| 2دانشیار گروه علوم دامی، دانشکده کشاوزی، دانشگاه زابل، زابل، ایران | ||
| 3دانشیار گروه علوم دامی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران | ||
| چکیده | ||
| نشان داده شده است که بروز هر گونه تغییر در عملکرد ژنهای DNMTs اثرات شگرفی بر فرآیند تکوین جنین و نیز وزن تولد در پستانداران دارد، لذا هدف از انجام این تحقیق بررسی چندشکلی ژنهای خانواده DNA متیل ترانسفراز و ارتباط آنها با وزن تولد در گاو سیستانی بود. خونگیری به طور تصادفی از 60 رأس گاو سیستانی به عمل آمد. استخراج DNA با استفاده از روش فنولکلروفرم انجام شد. نواحی کاندیدا برای وجود جهش شامل قطعههای 114 جفت بازی واقع در اگزون 33 ژن DNMT1، قطعه 176 جفت بازی واقع در اینترون 4 ژن DNMT3a و قطعه 207 جفت بازی واقع در اینترون 3 ژن DNMT3b توسط واکنش زنجیرهای پلیمراز تکثیر شدند. به منظور بررسی وجود چندشکلی در ژنهای هدف از روش PCR-SSCP بهوسیلهی ژل پلی آکریل آمید و رنگآمیزی نیترات نقره استفاده گردید. ارزیابی نتایج دلالت بر عدم وجود تنوع الگوهای باندی در تمام نمونههای مورد بررسی داشت. بر اساس نتایج این مطالعه به نظر میرسد که عدم مشاهده تنوع ژنتیکی در نواحی مورد بررسی احتمالاً دلالت بر انتخاب بر علیه وقوع جهش های خاص در جمعیت مورد نظر دارد. در مجموع براساس نتایج این مطالعه ارزیابی تنوع ژنتیکی در نواحی مورد بررسی، جهت شناسائی مارکرهای موثر در برنامههای اصلاحی صفات تولیدی در گاو سیستانی فاقد کارائی می باشد | ||
| کلیدواژهها | ||
| ژنهای DNA متیل ترانسفراز؛ چند شکلی؛ گاو سیستانی؛ PCR-SSCP | ||
| عنوان مقاله [English] | ||
| Analysis of polymorphism in DNMTS genes in Sistani cattle Using PCR-SSCP Method | ||
| نویسندگان [English] | ||
| Sajjad Shahdadi Sardo1؛ Mehdi Vafaye Valleh2؛ Gholam Reza Dashab3؛ Mohammad Rokouei3 | ||
| 1M.Sc, Department of Animal Science, Faculty of Agriculture, University of Zabol, Zabol, Iran | ||
| 2Department of Animal Science, Faculty of Agriculture, University of Zabol, Zabol, Iran | ||
| 3Associate Professor of University of Zabol | ||
| چکیده [English] | ||
| It has been shown that the occurrence of any changes in the function of DNMTs genes has a significant effect on both the embryo development and birth weight in mammals; therefore, the aim of the present study was to investigate the presence of DNA polymorphisms in the members of DNA methyl transferase superfamily and its relationship with birth weight in Sistani cattle’s. Blood sampling was done randomly from 60 Sistani cows. DNA extraction was performed using phenol chloroform method. Candidate region for the presence of functional polymorphism within the DNMTs gene including the 114-bp fragment of the exon 33 of the DNMT1 gene, the 176-bp fragment of the intron 4 of the DNMT3a gene, and the 207-bp fragment in intron 3 of the DNMT3b gene were amplified by polymerase chain reaction. PCR-SSCP followed by polyacrylamide gels silver stain analysis was done to evaluate the presence of candidate mutations in target gens. The result analysis of all analyzed region did not show any mutations in the investigated DNMTs genes. Based on the results of this study, it seems that the lack of observation of genetic diversity in the studied regions could be related to the evolutionary selection against occurrence of specific mutations in the analyzed population. In general, based on the results of this study, analysis of genetic diversity in the studied genomic region may not be effective in identifying effective markers for breeding programs in Sistani cows. | ||
| کلیدواژهها [English] | ||
| DNA Methyltransferases Genes, polymorphism, Sistani cattle, PCR-SSCP | ||
| مراجع | ||
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