| تعداد نشریات | 287 |
| تعداد نشریات سازمان | 85 |
| تعداد شمارهها | 3,159 |
| تعداد مقالات | 35,108 |
| تعداد مشاهده مقاله | 50,531,839 |
| تعداد دریافت فایل اصل مقاله | 90,480,600 |
Effects of Metformin on Experimental Varicocele in Rats | ||
| Archives of Razi Institute | ||
| مقاله 20، دوره 76، شماره 2، شهریور 2021، صفحه 371-384 اصل مقاله (1.49 M) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2020.128136.1406 | ||
| نویسندگان | ||
| H Karimi1؛ A Asghari* 1؛ A Jahandideh1؛ Gh Akbari1؛ P Mortazavi2 | ||
| 1Department of Clinical Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 2Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| چکیده | ||
| The current study aimed to determine the effect of metformin (MET) on histopathologic evaluation and antioxidant enzyme activity in experimental varicocele-induced rats. A total of 60 rats were randomly divided into six experimental groups. Group 1 (control) received no medication and underwent no surgery. In group 2 (sham), the rats received no medication and the abdominal cavity was opened; however, there was no varicocele induction. In group 3 (varicocele), the abdominal cavity was opened and the rats underwent varicocele induction and received no medication. In group 4, the abdominal cavity was opened and the animals received 25 mg/kg of MET for 42 days and were varicocele-induced. Groups 5 and 6 were similar to group 4 except that the animals received 50 and 100 mg/kg of MET, respectively. At the end of the 21st and 42nd days, the rats were euthanized and the left testis was removed for histological analysis and measurement of superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GPx), and total antioxidant status levels. According to the results, a dose-dependent difference was observed in testis damage grade in the MET treated groups, compared to that reported for the varicocele group (p <0.05). No difference was observed between 25 and 50 mg/kg of MET (P>0.05). Tissue MDA levels significantly increased in varicocele rats (p <0.05); however, MET (25, 50, and 100 mg/kg) in a dose-dependent manner decreased varicocele-induced MDA (p <0.05). Experimental varicocele significantly decreased SOD activity, compared to that reported for the control group (p <0.05). The administration of MET (25, 50, and 100 mg/kg) significantly increased tissue SOD activity in varicocele rats (p <0.05). The MET (25, 50, and 100 mg/kg) in a dose-dependent manner increased GPx activity in varicocele rats (p <0.05). There was no difference in MDA, SOD, and GPx levels between 25 and 50 mg/kg MET groups (P>0.05). The aforementioned findings suggested that MET treatment had beneficial effects on varicocele. | ||
| کلیدواژهها | ||
| Antioxidant؛ Histologic evaluation؛ Metformin؛ Varicocele؛ Rat | ||
| عنوان مقاله [English] | ||
| اثرات متفورمین بر واریکوسل تجربی در موش | ||
| چکیده [English] | ||
| هدف از مطالعه حاضر بررسی اثرات متفورمین بر ارزیابی هیستوپاتولوژی و فعالیت آنزیمهای آنتی اکسیدان در واریکوسل تجربی موش بود. شصت موش صحرایی نر بطور تصادفی به 6 گروه آزمایشی تقسیم شدند. گروه 1 بعنوان کنترل (بدون تزریق، جراحی و درمان) بودند. گروه دوم محوطه شکمی باز اما بدون ایجاد واریکوسل بخیه زده شد وتحت درمان قرار نگرفتند. گروه 3، واریکوسل تجربی در آنها انجام گرفت اما تحت درمان قرار نگرفتند. گروه 4، موشها پس از واریکوسل تجربی، به مدت 42 روز تحت درمان 25 میلیگرم بر کیلوگرم متفورمین قرار گرفتند. گروه 5 و 6 مشابه گروه 4 بود اما حیوانات سطوح 50 و 100 میلیگرم بر کیلوگرم متفورمین را دریافت کردند. در انتهای روز 21 و 42، حیوانات توسط آسانکشی بیضه چپ برای ارزیابی پاتولوژی و فعالیت آنزیمها مالون دی آلدهید، گلوتاتیون پراکسیداز، سوپرااکسیددسموتاز و فعالیت آنتیاکسیدان کل برداشت شد. براساس نتایج، متفورمین اثرات وابسته به دوز در آسیب بافتی متعاقب واریکوسل تجربی نشان داد (p < 0.05). همچنین تفاوت معنیداری در مقادیر 25 و 50 میلیگرم متفورمین دیده نشد (P>0.05). مقادیر بافتی مالون دی آلدهید بطور معنیداری در موشهای دچار واریکوسل افزایش داشت (p < 0.05) درحالیکه متفورمین (25، 50 و 100 میلیگرم بر کیلوگرم) بصورت وابسته به دوز موجب کاهش مقادیر مالون دی آلدهید ناشی از واریکوسل شد (p < 0.05). تجویز متفورمین(25، 50 و 100 میلیگرم بر کیلوگرم) موجب افزایش فعالیت سوپرااکسیددسموتاز ناشی از واریکوسل شد (p < 0.05). تجویز متفورمین(25، 50 و 100 میلیگرم بر کیلوگرم) موجب افزایش فعالیت سوپرااکسیددسموتاز ناشی از واریکوسل شد (p < 0.05). تفاوت معنیداری در مقادیر مالون دی آلدهید، سوپرااکسید دسموتاز و گلوتاتیون پراکسیداز بین گروه 25 و 50 میلیگرم بر کیلوگرم دیده نشد (P>0.05). نتایج نشان دهنده این است که متفورمین اثرات سودمندی بر واریکوسل دارد. | ||
| کلیدواژهها [English] | ||
| متفورمین, اریابی هیستولوژیکی, آنتی اکسیدان, واریکوسل, موش صحرایی | ||
|
سایر فایل های مرتبط با مقاله
|
||
| مراجع | ||
|
Agarwal, A., Durairajanayagam, D., Halabi, J., Peng, J., Vazquez-Levin, M., 2014. Proteomics, oxidative stress and male infertility. Reprod Biomed Online 29, 32-58.
Agarwal, A., Makker, K., Sharma, R., 2008. Clinical relevance of oxidative stress in male factor infertility: an update. Am J Reprod Immunol 59, 2-11.
Agarwal, A., Sharma, R.K., Desai, N.R., Prabakaran, S., Tavares, A., Sabanegh, E., 2009. Role of oxidative stress in pathogenesis of varicocele and infertility. Urology 73, 461-469.
Bertoldo, M.J., Guibert, E., Tartarin, P., Guillory, V., Froment, P., 2014. Effect of metformin on the fertilizing ability of mouse spermatozoa. Cryobiology 68, 262-268.
Celik-Ozenci, C., Bayram, Z., Akkoyunlu, G., Korgun, E.T., Erdogru, T., Seval, Y., et al., 2006. Localization of NGF and nNOS in varicocele-induced rat testis. Acta Histochem 107, 435-442.
Esfahanian, N., Shakiba, Y., Nikbin, B., Soraya, H., Maleki-Dizaji, N., Ghazi-Khansari, M., et al., 2012. Effect of metformin on the proliferation, migration, and MMP-2 and -9 expression of human umbilical vein endothelial cells. Mol Med Rep 5, 1068-1074.
Faure, M., Guibert, E., Alves, S., Pain, B., Rame, C., Dupont, J., et al., 2016. The insulin sensitiser metformin regulates chicken Sertoli and germ cell populations. Reproduction 151, 527-538.
Ghasemnejad-Berenji, M., Ghazi-Khansari, M., Yazdani, I., Nobakht, M., Abdollahi, A., Ghasemnejad-Berenji, H., et al., 2018. Effect of metformin on germ cell-specific apoptosis, oxidative stress and epididymal sperm quality after testicular torsion/detorsion in rats. Andrologia 50, e12846.
Gungor-Ordueri, N., Erdem, E., Karacan, M., Usta, A., 2019. Metformin Reduces the Extent of Varicocele-Induced Damage in Testicular Tissue. Glob J Reprod Med 6, 70-76.
Hirsch, H.A., Iliopoulos, D., Struhl, K., 2013. Metformin inhibits the inflammatory response associated with cellular transformation and cancer stem cell growth. Proc Natl Acad Sci USA 110, 972-977.
Hwang, K., Lamb, D.J., 2012. Molecular Mechanisms of Antioxidants in Male Infertility. In: Parekattil, S.J., Agarwal, A. (Eds.), Male Infertility: Contemporary Clinical Approaches, Andrology, ART & Antioxidants, Springer New York, New York, NY, pp. 45-54.
Johnsen, S.G., 1970. Testicular biopsy score count--a method for registration of spermatogenesis in human testes: normal values and results in 335 hypogonadal males. Hormones 1, 2-25.
Masson, P., Brannigan, R.E., 2014. The varicocele. Urol Clin North Am 41, 129-144.
Miller, N.J., Rice-Evans, C., Davies, M.J., Gopinathan, V., Milner, A., 1993. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci (Lond) 84, 407-412.
Morales, A.I., Detaille, D., Prieto, M., Puente, A., Briones, E., Arevalo, M., et al., 2010. Metformin prevents experimental gentamicin-induced nephropathy by a mitochondria-dependent pathway. Kidney Int 77, 861-869.
Morgante, G., Tosti, C., Orvieto, R., Musacchio, M.C., Piomboni, P., De Leo, V., 2011. Metformin improves semen characteristics of oligo-terato-asthenozoospermic men with metabolic syndrome. Fertil Steril 95, 2150-2152.
Oishi, N., Kendall, A., Schacht, J., 2014. Metformin protects against gentamicin-induced hair cell death in vitro but not ototoxicity in vivo. Neurosci Lett 583, 65-69.
Paglia, D.E., Valentine, W.N., 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70, 158-169.
Paoletti, F., Mocali, A., 1990. [18] Determination of superoxide dismutase activity by purely chemical system based on NAD(P)H oOxidation. Methods in Enzymology, Academic Press, pp. 209-220.
Sahin, Z., Bayram, Z., Celik-Ozenci, C., Akkoyunlu, G., Seval, Y., Erdogru, T., et al., 2005. Effect of experimental varicocele on the expressions of Notch 1, 2, and 3 in rat testes: an immunohistochemical study. Fertil Steril 83, 86-94.
Sahu, B.D., Kuncha, M., Putcha, U.K., Sistla, R., 2013. Effect of metformin against cisplatin induced acute renal injury in rats: a biochemical and histoarchitectural evaluation. Exp Toxicol Pathol 65, 933-940.
Schramm, T.K., Gislason, G.H., Vaag, A., Rasmussen, J.N., Folke, F., Hansen, M.L., et al., 2011. Mortality and cardiovascular risk associated with different insulin secretagogues compared with metformin in type 2 diabetes, with or without a previous myocardial infarction: a nationwide study. Eur Heart J 32, 1900-1908.
Soraya, H., Esfahanian, N., Shakiba, Y., Ghazi-Khansari, M., Nikbin, B., Hafezzadeh, H., et al., 2012. Anti-angiogenic Effects of Metformin, an AMPK Activator, on Human Umbilical Vein Endothelial Cells and on Granulation Tissue in Rat. Iran J Basic Med Sci 15, 1202-1209.
Tartarin, P., Moison, D., Guibert, E., Dupont, J., Habert, R., Rouiller-Fabre, V., et al., 2012. Metformin exposure affects human and mouse fetal testicular cells. Hum Reprod 27, 3304-3314.
Tek, M., Cayan, S., Yilmaz, N., Oguz, I., Erdem, E., Akbay, E., 2009. The effect of vascular endothelial growth factor on spermatogenesis and apoptosis in experimentally varicocele-induced adolescent rats. Fertil Steril 91, 2247-2252.
Turner, T.T., 2001. The study of varicocele through the use of animal models. Hum Reprod Update 7, 78-84.
Yan, W.J., Mu, Y., Yu, N., Yi, T.L., Zhang, Y., Pang, X.L., et al., 2015. Protective effects of metformin on reproductive function in obese male rats induced by high-fat diet. J Assist Reprod Genet 32, 1097-1104.
Zhang, H., Zhou, Q.M., Li, X.D., Xie, Y., Duan, X., Min, F.L., et al., 2006. Ginsenoside R(e) increases fertile and asthenozoospermic infertile human sperm motility by induction of nitric oxide synthase. Arch Pharm Res 29, 145-151.
Zhou, G., Myers, R., Li, Y., Chen, Y., Shen, X., Fenyk-Melody, J., et al., 2001. Role of AMP-activated protein kinase in mechanism of metformin action. J Clin Invest 108, 1167-1174.
Zimmermann, M., 1983. Ethical guidelines for investigations of experimental pain in conscious animals. Pain 16, 109-110. | ||
|
آمار تعداد مشاهده مقاله: 2,189 تعداد دریافت فایل اصل مقاله: 3,466 |
||
