| تعداد نشریات | 285 |
| تعداد نشریات سازمان | 83 |
| تعداد شمارهها | 3,094 |
| تعداد مقالات | 34,549 |
| تعداد مشاهده مقاله | 49,442,072 |
| تعداد دریافت فایل اصل مقاله | 79,874,026 |
Effect of Carum copticum nano-essence against Saprolegnia and Fusarium, and the Use of Multiplex PCR Assay for the Detection of These Organisms in Rainbow trout Oncorhynchus mykiss | ||
| Archives of Razi Institute | ||
| مقاله 7، دوره 76، شماره 2، شهریور 2021، صفحه 231-241 اصل مقاله (596.24 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2020.342066.1450 | ||
| نویسندگان | ||
| P Tour SavadKouhi1؛ H Ahari* 1؛ A. A Anvar2؛ B Jafari3 | ||
| 1Department of Food Sciences and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 2Department of Food Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 3Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| چکیده | ||
| This study aimed to investigate the fungal species isolation and confirmation by the Multiplex PCR method in aquatic fish. Evaluation of the inhibitory effect of nano-essential oils of Carum copticum on isolated fungal species was also conducted in this study. The PCR results showed that 3 out of 5 samples were diagnosed with Fusarium solani, and two of them were positive for Saprolegnia. Moreover, in 0.1% of the females' nanoparticles, one peak appeared that showed a particle with an average diameter of 360 nm, and two nanoparticles showed a peak with a mean diameter of 242 nm. The results of minimum inhibitory concentrations (MIC) and minimum fungicidal concentrations (MFC) showed that 0.01% nano essential oil had 0.08 and 0.07 mg/ml MIC values against Fusarium solani and Saprolegnia, respectively. Gram/ml was on the growth of Fusarium solani species. The essential oils of female plants had an MIC of 0.07 in 0.1% essential oil and 0.03 mg/ml in 0.01% essential oil in Saprolegnia. Furthermore, in the case of 0.1% nano essential oil, the results showed the MIC values of 0.04 and 0.03 mg/ml against Fusarium solani and Saprolegnia, respectively. The MFC values of 0.1% nano essential oil were 0.1 and 0.07 mg/ml against Fusarium solani and Saprolegnia,respectively. It was not found on Fusarium and Saprolegnia. Overall, the results of this study using PCR for direct detection showed that 70% and 50% of the samples were Fusarium solani and Saprolegnia positive, respectively; therefore, the PCR was an efficient method for the detection of fungi. According to the results of nano-essential oil (0.1%) of females, this nano-essence had a strong inhibitory effect on Fusarium solani and Saprolegnia. | ||
| کلیدواژهها | ||
| Carum copticum؛ Fusarium؛ Multiplex PCR؛ Nano-essence؛ Oncorhynchus mykiss؛ Saprolegnia | ||
| عنوان مقاله [English] | ||
| . | ||
|
سایر فایل های مرتبط با مقاله
|
||
| مراجع | ||
|
Boskabady, M.H., Alitaneh, S., Alavinezhad, A., 2014. Carum copticum L.: a herbal medicine with various pharmacological effects. Biomed Res Int 2014, 569087.
Chahal, K., Dhaiwal, K., Kumar, A., Kataria, D., Singla, N., 2017. Chemical composition of Trachyspermum ammi L. and its biological properties: A review. J Pharmacogn Phytochem 6, 131-140.
Daouk, R.K., Dagher, S.M., Sattout, E.J., 1995. Antifungal Activity of the Essential Oil of Origanum syriacum L. J Food Prot 58, 1147-1149.
Gevezova, M., Tserkova, F., Kirilova, I., Hubenova, T., Zaikov, A., Ivanova, A., et al., 2017. Potential of PCR-based Molecular Diagnostic Methods for Rapid Detection of Bacterial Pathogens Causing Economically Important Diseases in Fishes. Acta Zoologica Bulgarica Supplementum, 8, 37-40.
Ghiasi, M., Khosravi, A., Soltani, M., Sharifpour, I., Binaii, M., Ebrahimzadeh Mosavi, H., et al., 2014. Evaluation of physiological aspects and molecular identification of Saprolegnia isolates from rainbow trout (Oncorhynchus mykiss) and Caspian trout (Salmo trutta caspius) eggs based on RAPD–PCR. Iran Sci Fish J 22, 82-92.
Goudarzi, G.R., Saharkhiz, M., Sattari, M., Zomorodian, K., 2011. Antibacterial activity and chemical composition of Ajowan (Carum copticum Benth. & Hook) essential oil. J Agric Sci Technol 13, 203-208.
Hadian, J., Hekmati, M., Ghorbanpour, M., 2016. Agromorphological Variations and Essential Oil Production of Satureja khuzestanica Jamzad under Different Planting Densities. J Essent Oil-Bear Plants 19, 1102-1110.
Hooshangi, S., Firouzbakhsh, F., Badalii, H., 2016. Evaluation of Invitro Antifungal Activity of Mentha Piperita and Carum Capticum against Saprolegnia Parasitica. J Vet Microbiol 12, 47-55.
Mobaiyen, H., Nasarollah Pour, M., Elmi, F., 2015. Phytochemical Composition and Antibacterial Activity of Trachyspermum copticum L. essential oil, East Azerbaijan, Iran. J Med Microbiol Infect Dis 3, 71-74.
Nagaraja, H., Chennappa, G., Rakesh, S., Naik, M.K., Amaresh, Y.S., Sreenivasa, M.Y., 2016. Antifungal activity of Azotobacter nigricans against trichothecene-producing Fusarium species associated with cereals. Food sci biotechnol 25, 1197-1204.
Niessen, L., Schmidt, H., Vogel, R.F., 2004. The use of tri5 gene sequences for PCR detection and taxonomy of trichothecene-producing species in the Fusarium section Sporotrichiella. Int J Food Microbiol 95, 305-319.
Raeisi, S., Sharifi-Rad, M., Quek, S.Y., Shabanpour, B., Sharifi-Rad, J., 2016. Evaluation of antioxidant and antimicrobial effects of shallot (Allium ascalonicum L.) fruit and ajwain (Trachyspermum ammi (L.) Sprague) seed extracts in semi-fried coated rainbow trout (Oncorhynchus mykiss) fillets for shelf-life extension. LWT-Food Sci Technol 65, 112-121.
Rodríguez-Tudela, J.L., Barchiesi, F., Bille, J., Chryssanthou, E., Cuenca-Estrella, M., Denning, D., et al., 2003. Method for the determination of minimum inhibitory concentration (MIC) by broth dilution of fermentative yeasts. Clin Microbiol Infect 9, 1-8.
Salimian, F., Fattollahi, M., Nematollahi, A., Nikookhah, F., Kharazian, N., 2017. Antifungal activity in vitro of aqueous and total flavnoids extracts of plant Myrtus communis L. against two pathogenically important fungi, Saprolegnia and Fusarrium isolated from rainbow trout eggs. J Mar Sci Technol 16, 16-28.
Shahbazian, N., Ebrahimzadeh Mousavi, H.A., Soltani, M., Khosravi, A.R., Mirzargar, S., Sharifpour, I., 2010. Fungal contamination in rainbow trout eggs in Kermanshah province propagations with emphasis on Saprolegniaceae. Iran J Fish Sci 9, 151-160.
Sim, C.S.F., Yue, C.S., Cheow, Y.L., Ting, A.S.Y., 2019. Influence of metal stress on production of volatile inhibitory compounds by endophytes against Ganoderma boninense. Biocontrol Sci Technol 29, 860-876.
Stakheev, A., Ryazantsev, D.Y., Gagkaeva, T.Y., Zavriev, S., 2011. PCR detection of Fusarium fungi with similar profiles of the produced mycotoxins. Food Control 22, 462-468.
Suanthie, Y., Cousin, M.A., Woloshuk, C.P., 2009. Multiplex real-time PCR for detection and quantification of mycotoxigenic Aspergillus, Penicillium and Fusarium. J Stored Prod Res 45, 139-145.
Tabassum Khan, N., Jameel, N., 2018. Antifungal Activity of Ajawain Seeds (Trachyspermum ammi). Biomol Res Ther 7, 2672-2674. Weisany, W., Samadi, S., Amini, J., Hossaini, S., Yousefi, S., Maggi, F., 2019. Enhancement of the antifungal activity of thyme and dill essential oils against Colletotrichum nymphaeae by nano-encapsulation with copper NPs. Ind Crop Prod 132, 213-225. | ||
|
آمار تعداد مشاهده مقاله: 2,247 تعداد دریافت فایل اصل مقاله: 2,869 |
||
