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Effect of Sodium Cyanide-induced Tissue Hypoxia on Reproductive Capability of Male Mice and the Protective Effect of Ethyl Pyruvate | ||
| Archives of Razi Institute | ||
| مقاله 16، دوره 76، شماره 2، شهریور 2021، صفحه 323-333 اصل مقاله (686.57 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2020.126806.1362 | ||
| نویسندگان | ||
| M. R Nourani* 1؛ A Kalantari Hesari2؛ R Shahrooz3؛ M. R Asadi4 | ||
| 1Genomics Division, Systems Biology Institute, Tissue Engineering Division, Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran | ||
| 2Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran | ||
| 3Professor of Histology and Embryology, Department of Basic Science, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran | ||
| 4Assistant Professor of Histology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran | ||
| چکیده | ||
| The present study aimed to assess the effect of tissue hypoxia induced by sodium cyanide (NaCN) on male mice fertility and the protective role of ethyl pyruvate (EP). A number of 30 adult mice were assigned to three groups: 1) a control group, 2) a treatment group treated with 2 mg/kg of NaCN, and 3) a treatment group treated with 2 mg/kg of NaCN, along with 40 mg/kg EP (NaCN+EP). After 35 days, animals were anesthetized and serum, sperm, and tissue samples were taken. The results demonstrated a significant decrease in sperm quality, reproduction potency, and anti-oxidant potential, as well as an increase in lipid peroxidation in the NaCN group (p <0.05). Moreover, the use of EP effectively restrained the disastrous effects of tissue hypoxia. It can be concluded that EP can moderate the complications resulting from tissue-hypoxia that is related to testes parameters. | ||
| کلیدواژهها | ||
| Ethyl pyruvate؛ Cyanide؛ Mice؛ Reproduction potency؛ Sodium؛ Tissue hypoxia | ||
| عنوان مقاله [English] | ||
| بررسی اثر هایپوکسی بافتی ناشی از سدیم سیانید بر توان باروری موش سوری نر و نقش محافظتی اتیل پیروات | ||
| چکیده [English] | ||
| کاهش میزان اکسیژن رسانی میتواند موجب اختلال در عملکرد بیضه و اسپرماتوژنز گردد. هدف این مطالعه بررسی اثر هیپوکسی بافتی ایجاد شده توسط سدیم سیانید بر توان باروری موش سوری نر و نقش محافظتی اتیل پیروات بر آسیب ناشی از این هیپوکسی بود. از این رو در تحقیق حاضر تعداد 30 سر موش سوری نر بالغ 25 تا 30 گرمی در 3 گروه مورد استفاده قرار گرفتند. گروه کنترل دریافت کننده سرم فیزیولوژی، گروه دریافت کننده سدیم سیانید با دوز mg/kg 2 هر 24 ساعت یک بار به صورت تزریق داخل صفاقی و گروه دریافت کننده سدیم سیانید با دوز mg/kg 2 به همراه اتیل پیروات با دوز mg/kg 40 به صورت تزریق روزانه داخل صفاقی به مدت 35 روز دریافت نمودند. پس از پایان دوره تجویز، پس از بیهوشی خونگیری از طریق قلب انجام و متعاقب آسان کشی نمونههای سرمی جهت آزمایشات سرمی، نمونههای اسپرم جهت آزمایشات بررسی اسپرم و IVF و نمونههای بافتی نیز پس از جدا سازی در داخل محلول بوئن فیکس شده و پس از انجام برشهای پارافینی با رنگ آمیزیهای هماتوکسیلین- ائوزین مورد بررسی هیستومورفومتری قرار گرفتند. نتایج نشان دهنده کاهش معنیدار کیفیت اسپرم و توان باروری، کاهش فاکتورهای TDI ، RI و SI و تعداد سلولهای لیدیگ، کاهش توان آنتی اکسیدانتی و افزایش میزان پراکسیداسیون چربیها درگروههای دریافت کننده سدیم سیانید بود (05/0>P). این در حالی بود که استفاده از اتیل پیروات به عنوان یک آنتی اکسیدان قوی به خوبی توانسته بود اثرات سوء حاصل از هیپوکسی بافتی را جبران کند. بنابراین میتوان نتیجه گرفت که اتیل پیروات قادر به خنثی نمودن عوارض ناشی از هیپوکسی بافتی در رابطه با پارامترهای بیضه میباشد. | ||
| کلیدواژهها [English] | ||
| هیپوکسی بافتی, سدیم سیانید, اتیل پیروات, توان باروری، موش | ||
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| مراجع | ||
|
Anseeuw, K., Delvau, N., Burillo-Putze, G., De Iaco, F., Geldner, G., Holmstrom, P., et al., 2013. Cyanide poisoning by fire smoke inhalation: a European expert consensus. Eur J Emerg Med 20, 2-9.
Ardelt, B.K., Borowitz, J.L., Isom, G.E., 1989. Brain lipid peroxidation and antioxidant protectant mechanisms following acute cyanide intoxication. Toxicology 56, 147-154.
Ardelt, B.K., Borowitz, J.L., Maduh, E.U., Swain, S.L., Isom, G.E., 1994. Cyanide-induced lipid peroxidation in different organs: subcellular distribution and hydroperoxide generation in neuronal cells. Toxicology 89, 127-137.
Assinder, S., Davis, R., Fenwick, M., Glover, A., 2007. Adult-only exposure of male rats to a diet of high phytoestrogen content increases apoptosis of meiotic and post-meiotic germ cells. Reproduction 133, 11-19.
Daya, S., Walker, R.B., Anoopkumar-Dukie, S., 2000. Cyanide-induced free radical production and lipid peroxidation in rat brain homogenate is reduced by aspirin. Metab Brain Dis 15, 203-210.
Dirikolu, L., Hughes, C., Harkins, D., Boyles, J., Bosken, J., Lehner, F., et al., 2003. The toxicokinetics of cyanide and mandelonitrile in the horse and their relevance to the mare reproductive loss syndrome. Toxicol Mech Methods 13, 199-211.
Elia, R., Elgoyhen, A.B., Bugallo, G., Río, M.E., Bozzini, C.E., 1985. Effect of acute exposure to reduced atmospheric pressures on body weight, food intake and body composition of growing rats. Acta Physiol Pharmacol Latinoam 35, 311-318.
Fahim, M.S., Messiha, F.S., Girgis, S.M., 1980. Effect of acute and chronic simulated high altitude on male reproduction and testosterone level. Arch Androl 4, 217-219.
Friedman, M.A., Staub, J., 1976. Inhibition of mouse testicular DNA synthesis by mutagens and carcinogens as a potential simple mammalian assay for mutagenesis. Mutat Res-Fund Mol M 37, 67-76.
Gosney, J.R., 1984. Effects of hypobaric hypoxia on the Leydig cell population of the testis of the rat. J Endocrinol 103, 59-62.
Guerin, P., El Mouatassim, S., Menezo, Y., 2001. Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Hum Reprod Update 7, 175-189.
Hashimoto, S., Minami, N., Yamada, M., Imai, H., 2000. Excessive concentration of glucose during in vitro maturation impairs the developmental competence of bovine oocytes after in vitro fertilization: Relevance to intracellular reactive oxygen species and glutathione contents. Mol Reprod Dev 56, 520-526.
Iwasaki, A., Gagnon, C., 1992. Formation of reactive oxygen species in spermatozoa of infertile patients**Supported by grant MT-6490 from the Medical Research Council of Canada (to C.G.). Fertil Steril 57, 409-416.
Kalantari-Hesari, A., Shahrooz, R., Ahmadi, A., Malekinejad, H., Saboory, E., 2015. Crocin prevention of anemia-induced changes in structural and functional parameters of mice testes. J Appl Biomed 13, 213-223.
Kheradmand, A., Alirezaei, M., Dezfoulian, O., 2013. Cadmium-Induced Oxidative Stress in the Rat Testes: Protective Effects of Betaine. Int J Pept Res Ther 19, 337-344.
Khmel'nitskii, O.K., Tararak, T.Y., 1991. Effect of exposure to high-altitude hypoxia on morphology of the pituitary-gonads system. Bull Exp Biol Med 111, 558-561.
Kowaltowski, A.J., Vercesi, A.E., 1999. Mitochondrial damage induced by conditions of oxidative stress. Free Radic Biol Med 26, 463-471.
Leese, H.J., Barton, A.M., 1985. Production of pyruvate by isolated mouse cumulus cells. J Exp Zool 234, 231-236.
Liao, W., Cai, M., Chen, J., Huang, J., Liu, F., Jiang, C., et al., 2010. Hypobaric hypoxia causes deleterious effects on spermatogenesis in rats. Reproduction 139, 1031-1038.
Mathangi, D.C., Namasivayam, A., 2000. Effect of chronic cyanide intoxication on memory in albino rats. Food Chem Toxicol 38, 51-55.
Morales, H., Tilquin, P., Rees, J.F., Massip, A., Dessy, F., Van Langendonckt, A., 1999. Pyruvate prevents peroxide-induced injury of in vitro preimplantation bovine embryos. Mol Reprod Dev 52, 149-157.
Mousavi, S.H., Tayarani, N.Z., Parsaee, H., 2010. Protective effect of saffron extract and crocin on reactive oxygen species-mediated high glucose-induced toxicity in PC12 cells. Cell Mol Neurobiol 30, 185-191.
NTP, 1993. NTP Toxicity Studies of Sodium Cyanide (CAS No. 143-33-9) Administered by Dosed Water to F344/N Rats and B6C3F1 Mice. Toxic Rep Ser 37, 1-3.
Okolie, N.P., Iroanya, C.U., 2003. Some histologic and biochemical evidence for mitigation of cyanide-induced tissue lesions by antioxidant vitamin administration in rabbits. Food Chem Toxicol 41, 463-469.
Park, J.D., Habeebu, S.S.M., Klaassen, C.D., 2002. Testicular toxicity of di-(2-ethylhexyl) phthalate in young Sprague–Dawley rats. Toxicology 171, 105-115.
Payabvash, S., Kiumehr, S., Tavangar, S.M., Dehpour, A.R., 2008. Ethyl pyruvate reduces germ cell-specific apoptosis and oxidative stress in rat model of testicular torsion/detorsion. J Pediatr Surg 43, 705-712.
Pieniazek, P.A., Bradforth, S.E., Krylov, A.I., 2006. Spectroscopy of the cyano radical in an aqueous environment. J Phys Chem A 110, 4854-4865.
Rezvanfar, M.A., Rezvanfar, M.A., Shahverdi, A.R., Ahmadi, A., Baeeri, M., Mohammadirad, A., et al., 2013. Protection of cisplatin-induced spermatotoxicity, DNA damage and chromatin abnormality by selenium nano-particles. Toxicol Appl Pharmacol 266, 356-365.
Saxena, D.K., 1995. Effect of hypoxia by intermittent altitude exposure on semen characteristics and testicular morphology of male rhesus monkeys. Int J Biometeorol 38, 137-140.
Shivanoor, S.M., David, M., 2014. Subchronic cyanide toxicity on male reproductive system of albino rat. Toxicol Res 4, 57-64.
Singh, J.D., 1981. The teratogenic effects of dietary Cassava on the pregnant albino rat: a preliminary report. Teratology 24, 289-291.
Soto-Blanco, B., Stegelmeier, B.L., Gorniak, S.L., 2005. Clinical and pathological effects of short-term cyanide repeated dosing to goats. J Appl Toxicol 25, 445-450.
Vander Heiden, M.G., Cantley, L.C., Thompson, C.B., 2009. Understanding the Warburg effect: the metabolic requirements of cell proliferation. Science 324, 1029-1033.
Verratti, V., Berardinelli, F., Di Giulio, C., Bosco, G., Cacchio, M., Pellicciotta, M., et al., 2008. Evidence that chronic hypoxia causes reversible impairment on male fertility. Asian J Androl 10, 602-606.
Vetter, J., 2000. Plant cyanogenic glycosides. Toxicon 38, 11-36.
Wang, Q., van Hoecke, M., Tang, X.N., Lee, H., Zheng, Z., Swanson, R.A., et al., 2009. Pyruvate protects against experimental stroke via an anti-inflammatory mechanism. Neurobiol Dis 36, 223-231.
Yang, R., Shaufl, A.L., Killeen, M.E., Fink, M.P., 2009. Ethyl pyruvate ameliorates liver injury secondary to severe acute pancreatitis. J Surg Res 153, 302-309. | ||
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