| تعداد نشریات | 287 |
| تعداد نشریات سازمان | 85 |
| تعداد شمارهها | 3,159 |
| تعداد مقالات | 35,108 |
| تعداد مشاهده مقاله | 50,530,659 |
| تعداد دریافت فایل اصل مقاله | 90,479,323 |
Fowlpox Outbreak in a laying Farm: Up to Date Data on Phylogenetic Analysis in Iran, 2018 | ||
| Archives of Razi Institute | ||
| مقاله 10، دوره 75، شماره 4، اسفند 2020، صفحه 501-508 اصل مقاله (396.7 K) | ||
| نوع مقاله: Case Study | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2019.124054.1269 | ||
| نویسندگان | ||
| M. H. Fallah Mehrabadi1؛ A. Ghalyanchilangeroudi* 2؛ S. Charkhkar3؛ H. Hosseini4؛ T. Zabihipetroudi2؛ A. Shayganmehr2؛ R. Esmaeelzadeh2؛ L. Aghaeean2 | ||
| 1Department of Poultry Diseases, of Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran | ||
| 2Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
| 3Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Tehran, Iran | ||
| 4Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Karaj Branch, Alborz, Iran | ||
| چکیده | ||
| Fowlpox is an economically significant viral disease in poultry, characterized by two forms of clinical signs, including cutaneous and diphtheritic lesions. This infection can have several adverse effects on flock performance, such as a reduction in egg production and growth and an increase in mortality. In winter 2018, an infection suspected to fowlpox was reported from a Hy-line W-36 laying farm in Isfahan province, Iran. The birds were 38 weeks of age and showed obvious diphtheritic signs in mucous membranes with increased mortality and reduced egg production. In total, 20 samples were collected from diphtheritic lesions (Trachea and Esophagus) of infected birds. The Polymerase Chain Reaction method was used to amplify a 578 bp fragment of the poxvirus 4b core protein gene. Phylogenetic relationships of avian poxviruses are usually analyzed using the 4b core protein-coding gene sequences with molecular weights of 75.2 kDa. The major elements had the fowlpox genome, and sequencing was performed for one isolate as representative. The nucleotide sequence result showed that this isolate (FP\UT-POX-2018) had a similarity rate of 99.53% with the previous Iranian fowlpox isolate (FP\GHPCRLAB.3) sequenced in the GenBank.Moreover, there was a 100% similarity among the current isolate nucleotide sequence, FP/NobilisVarioleW, and FP/FPV-VR250. The derived phylogenetic tree showed that these isolates were clustered in A1 subclades. Therefore, Iranian isolates of fowlpox virus have remained in the same subclade of phylogenetic classification (subclade A1), and they show high genomic similarity with previous isolates of Iran. Veterinarians and farmers must not underestimate fowlpox. However, they should consider the importance of vaccination against this disease like any other disease care. | ||
| کلیدواژهها | ||
| Fowlpox؛ Iran؛ Laying flock؛ PCR؛ Phylogenetic analysis | ||
|
سایر فایل های مرتبط با مقاله
|
||
| مراجع | ||
|
Adams, C.J., Feldman, S.H., Sleeman, J.M., 2005. Phylogenetic analysis of avian poxviruses among free-ranging birds of Virginia. Avian Dis 49, 601-605.
Afonso, C.L., Tulman, E.R., Lu, Z., Zsak, L., Kutish, G.F., Rock, D.L., 2000. The Genome of Fowlpox Virus. J Virol 74, 3815-3831.
Bolte, A.L., Meurer, J., Kaleta, E.F., 1999. Avian host spectrum of avipoxviruses. Avian Pathol 28, 415-432.
Gholami-Ahangaran, M., Zia-Jahromi, N., Namjoo, A., 2014. Molecular detection of avian pox virus from nodular skin and mucosal fibrinonecrotic lesions of Iranian backyard poultry. Trop Anim Health Prod 46, 349-353.
Gyuranecz, M., Foster, J.T., Dan, A., Ip, H.S., Egstad, K.F., Parker, P.G., et al., 2013. Worldwide phylogenetic relationship of avian poxviruses. J Virol 87, 4938-4951.
Jarmin, S., Manvell, R., Gough, R.E., Laidlaw, S.M., Skinner, M.A., 2006. Avipoxvirus phylogenetics: identification of a PCR length polymorphism that discriminates between the two major clades. J Gen Virol 87, 2191-2201.
Lee, L.H., Lee, K.H., 1997. Application of the polymerase chain reaction for the diagnosis of fowl poxvirus infection. J Virol Methods 63, 113-119.
Lüschow, D., Hoffmann, T., Hafez, H., 2004. Differentiation of avian poxvirus strains on the basis of nucleotide sequences of 4b gene fragment. Avian Dis 48, 453-462.
Manarolla, G., Pisoni, G., Sironi, G., Rampin, T., 2010. Molecular biological characterization of avian poxvirus strains isolated from different avian species. Vet Microbiol 140, 1-8.
Murphy, F., Gibbs, E., Horzineck, M., Studdert, M., 1999. Poxviridae. In: Gibbs, E., Horzinek, M., Studdert, M. (Eds.), Veterinary virology, Academic Press, San Diego, pp. 277–291.
Nayeri Fasaei, B., Madadgar, O., Ghalyanchi Langeroodi, A., Ghafari, M.M., 2014. Molecular detection and phylogenetic analysis of Avipoxvirus strains isolated from different bird species. Iran J Vet Res 15, 40-44.
Norouzian, H., Farjanikish, G., 2017. Pathological and molecular diagnosis of avian pox in backyard chickens in Khorram Abad. Vet Res Biol Prod 30, 2-10.
Tripathy ND, MR., W., 2013. POX. In: wayne D, G.J., McDougald L, Nolan L, Suarez D, Nair V, editors (Ed.), Diseases of Poultry, American Association of Avian Pathology, pp. 333–349.
Weli, S., Traavik, T., Tryland, M., Coucheron, D., Nilssen, Ø., 2004. Analysis and comparison of the 4b core protein gene of avipoxviruses from wild birds: evidence for interspecies spatial phylogenetic variation. Arch Virol 149, 2035-2046.
Zimmermann, D., Anderson, M.D., Lane, E., van Wilpe, E., Carulei, O., Douglass, N., et al., 2011. Avian poxvirus epizootic in a breeding population of Lesser Flamingos (Phoenicopterus minor) at Kamfers Dam, Kimberley, South Africa. J Wildl Dis 47, 989-993. | ||
|
آمار تعداد مشاهده مقاله: 16,264 تعداد دریافت فایل اصل مقاله: 66,680 |
||
