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Genetic Affinity of Echinococcus granulosus protoscolex in Human and Sheep in East Azerbaijan, Iran | ||
| Archives of Razi Institute | ||
| مقاله 9، دوره 75، شماره 1، خرداد 2020، صفحه 47-54 اصل مقاله (699.94 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2018.122733.1227 | ||
| نویسندگان | ||
| S. Zarrabi Ahrabi1؛ R. Madani* 1، 2؛ B. Shemshadi1؛ Sh. Ranjbar Bahadori3؛ H. Hashemzadeh Farhang4 | ||
| 1Department of Microbiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 2Department of Proteomics & Biochemistry section Biotechnology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| 3Department of Parasitology, Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Semnan, Iran | ||
| 4Department of Parasitology, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran | ||
| چکیده | ||
| Echinococcosis caused by the larval form of Echinococcus granulosus (E. granulosus) is known as an important zoonotic disease in various parts of the world, including Iran. The genetic diversity of this parasite is very high, particularly in areas where the disease is endemic. It has been suggested in the literature from different parts of the world that diverse factors, such as parasite life cycle, transmission pathways, pathologic disease, immunization, and disease control can be affected by the genetic diversity of the parasite. Various studies indicated sheep strain G1 as the most common genotype throughout the world. This strain is commonly found in the liver and lung repeatedly causing echinococcosis in humans, sheep, and cattle. The present study was conducted to determine the genetic affinity between the protoscolex of E. granulosus in humans and sheep in East Azerbaijan province, Iran for the first time. A total of 120 hydatid cyst samples were collected, 60 of which were from people who referred to the hospitals of East Azerbaijan and 60 were from the sheep slaughtered in Tabriz slaughterhouse. Following DNA extraction, certain regions of the cox1 gene were amplified and evaluated by the polymerase chain reaction. The replicated parts in all isolates had the same size of 450 bp. Electrophoresis was followed by selecting a total of 60 suitable samples, including 30 human samples and 30 sheep samples and sending them for genome sequencing. The overlap of the samples was investigated using the BLAST software. The results of BLAST, sequencing, and overlap demonstrated a genetic linkage of approximately 91.76% between the protoscolex of E. granulosus in human and sheep. | ||
| کلیدواژهها | ||
| DNA extraction؛ Genetic affinity؛ Hydatid cyst؛ PCR؛ Sequencing | ||
| عنوان مقاله [English] | ||
| تعیین قرابت ژنتیکی پروتواسکولکس اکینوکوکوس گرانولوزوس در انسان و گوسفند دراستان آذربایجان شرقی | ||
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