motamed, N., shoushtar, H., Fallah Mehrabadi, M., Yousefi Amin, A. (2020). Development of Neuraminidase Inhibition test for subtyping avian influenza viruse Neuraminidase, in Razi Vaccine and Serum Research Institute. , 33(1), 2-8. doi: 10.22092/vj.2019.123215.1491
najmeh motamed; Hamid shoushtar; Mohammad Hossien Fallah Mehrabadi; Asghar Yousefi Amin. "Development of Neuraminidase Inhibition test for subtyping avian influenza viruse Neuraminidase, in Razi Vaccine and Serum Research Institute". , 33, 1, 2020, 2-8. doi: 10.22092/vj.2019.123215.1491
motamed, N., shoushtar, H., Fallah Mehrabadi, M., Yousefi Amin, A. (2020). 'Development of Neuraminidase Inhibition test for subtyping avian influenza viruse Neuraminidase, in Razi Vaccine and Serum Research Institute', , 33(1), pp. 2-8. doi: 10.22092/vj.2019.123215.1491
motamed, N., shoushtar, H., Fallah Mehrabadi, M., Yousefi Amin, A. Development of Neuraminidase Inhibition test for subtyping avian influenza viruse Neuraminidase, in Razi Vaccine and Serum Research Institute. , 2020; 33(1): 2-8. doi: 10.22092/vj.2019.123215.1491

Development of Neuraminidase Inhibition test for subtyping avian influenza viruse Neuraminidase, in Razi Vaccine and Serum Research Institute

تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
Article 1, Volume 33, Issue 1, March 2020, Pages 2-8    PDF (748.03 K)
Document Type: Full Research Paper
DOI: 10.22092/vj.2019.123215.1491
Authors
najmeh motamed* 1; Hamid shoushtar2; Mohammad Hossien Fallah Mehrabadi2; Asghar Yousefi Amin3
1Assistant professor of Department of Poultry Diseases Vaccine and Research, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREO) Karaj-Iran.
2Assistant professor of Department of Poultry Diseases Research, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREO) Karaj-Iran.
3Department of Poultry Diseases Research, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization(AREO) Karaj-Iran.
Abstract
Detection and identification of influenza A viruses hemagglutinin is done with serological or molecular techniques and the neuraminidase (NA) subtyping is also necessary in purpose of epidemiological studies specially in H5 or H7 (highly pathogenic/ low pathogenic) outbreaks and/or endemic areas. Subtype Identification of NA usually is performed by molecular tests such as RT-PCR. Since 1971, WHO has suggested that the surface glycoprotein neuraminidase of avian influenza viruses could be divided in different subtypes serologically by Neuraminidase Inhibition test (NI). In reference or specialized laboratories like OIE licensed labs, NI test is running routinely. Considering the importance of this valuable test, an experimental study was conducted to develop the classic NI test according to OIE protocol. RT-PCR test with subtype-specific primers was used as a control for evaluation of test accuracy. According to our results, NI test can detect and distinguish various NA subtypes in serum or viral samples. In conclusion, the NI test is a practical tool in diagnostic and research trials, especially in differentiation of vaccinated from unvaccinated birds (DIVA) in endemic areas with vaccination strategy or in validated reference laboratories such as Razi Vaccine and Serum Research Institute.
Keywords
Neuraminidase Inhibition; influenza virus; RT-PCR; Reference Laboratories
References

1. Aminoff, D .1961. Methods for the Quantitative Estimation of N-Acetylneuraminic Acid and their Application to Hydrolysates of Sialomucoids. Biochemistry Journal 81: 384-392
2. Aymard-Henry, M., M. T. Coleman, W. R. Dowdle, W. G. Laver, G. C. Schild, and R. G. Webster.1973.Influenzavirus neuraminidase and neuraminidase-inhibition test procedures. Bulltein of world Health organization 48: 199-202
3. Capua, I., C. Terregino, G. Cattoli, F. Mutinelli and J. F. Rodriguez. 2003. Development of a DIVA (Differentiating Infected from Vaccinated Animals) strategy using a vaccine containing a heterologous neuraminidase for the control of avian influenza. Avian Pathology 32: 47-55
4. De Benedictis, P., M. S. Beato and I. Capua. 2007. Inactivation of avian influenza viruses by chemical agents and physical conditions: a review. Zoonoses Public Health 54:51–68.
5. De Flora, S. and G. Badolati. 1973. Thermal inactivation of untreated and gamma-irradiated A2-Aichi-2-68 influenza virus. Journal of Genetic Virology 20:261–26
6. Fereidouni, S. R., E. Starick, C. Grund, A. Globig, T.C. Mettenleiter, M. Beer and T. Harder.2009. Rapid molecular subtyping by reverse transcription polymerase chain reaction of the neuraminidase gene of avian influenza A viruses. Veterinary Microbiology 135:253-260
7. Goldstein, M. A. and N. M. Tauraso. 1970. Effect of formalin, _-propiolactone, merthiolate, and ultraviolet light upon influenza virus infectivity, chicken cell agglutination, hemagglutination, and antigenicity. Applied Microbiology 19:290–294
8. Jonges M, W. M. Liu , E. vander Vries , R. Jacobi, Pronk I, C. Boog , M. Koopmans , A. Meijer and E. Soethout .2010.Influenza Virus Inactivation for Studies of Antigenicity and Phenotypic Neuraminidase Inhibitor Resistance Profiling. Journal of Clinical Microbiology 48:928-40.
9. King, D. J. 1991. Evaluation of different methods of inactivation of Newcastle disease virus and avian influenza virus in egg fluids and serum. Avian Disease 35:505–514.
10. Murphy, B. R., J. A. Kasel and R.M. Chanock.1972. Association of serum anti-neuraminidase antibody with resistance to influenza in man. The New England Journal of Medicine 286: 1329-1332.
11. OIE.2016. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals.Chapter 2.3. 4.Avian influenza. Available on line : https://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.03.04_AI.pdf accessed at: 2018/08/31
12. Sandbulte, M. R., J. Gao, T. M. Straight and M. C. Eichelberger.2009. A miniaturized assay for influenza neuraminidase inhibiting antibodies utilizing reverse genetics-derived antigens. Influenza and Other Respiratory Viruses 3: 233–240
13. Schulman, J. L., M. Khakpour and E. D. Kilbourne.1968. Protective effects of specific immunity to viral neuraminidase on influenza virus infection of mice. Journal of Virology 2: 778-786.
14. Shtyrya, Y. A., L. V. Mochalova and N. V. Bovin.2009.Influenza Virus Neuraminidase: Structure and Function. Acta Nature 2:26-32
15. Terregino, C. and I. Capua.2009. Conventional diagnosis of avian influenza.pp.73-86, In: Capua, I., and D. Alexander(ed), Avian Influenza and Newcastle Disease, Afield and Laboratory manual. Springer-verlag, Milan.
16. Tsukamoto, K., T., Ashizawa, K., Nakanishi, N., Kaji, K., Suzuki, Shishido, M., Okamatsu, M. and Masaji Mas. 2009. Use of Reverse Transcriptase PCR to Subtype N1 to N9 Neuraminidase Genes of Avian Influenza Viruse. Journal of Clinical Microbiology 47: 2301-2303
17. Vatandour, S., M. Bozorgmehrifard, H. Shoushtari, S. Charkhkar and S. Bakhtiari. 2011. Molecular characterization and phylogenetic analysis of neuraminidase gene of avian Influenza H9N2 viruses isolated from commercial broiler chicken in Iran during a period of 1998-2007. African Journal of Microbiology Research 5: 4182-4189.

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