Toxicity of Hottentotta saulcyi venom against Sesamia nonagrioides on sugarcane plants | ||
| آفات و بیماری های گیاهی | ||
| Article 13, Volume 87, Issue 1 - Serial Number 108, September 2019, Pages 1-12 PDF (613.11 K) | ||
| Document Type: Agricultural Entomology | ||
| DOI: 10.22092/jaep.2019.122681.1240 | ||
| Authors | ||
| fatemeh salabi* 1; Mahmood Nazari2; Hadiye Jafari3; Ali Shahriari4; Anahita Rezaei5 | ||
| 1Razi Vaccine and Serum Research Institute | ||
| 2Department of Animal Science, Faculty of Animal science and Food Technology, Khuzestan University of Agricultural Sciences and Natural Resources, Ahvaz, Iran | ||
| 3Department of Venomous Animals and Anti-venom Production, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Ahvaz, Iran | ||
| 4Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| 5Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| Abstract | ||
| To evaluate the toxicity of Hottentotta saulcyi venom on Sesamia nonagrioides, we investigated the larval mortality, detoxification enzyme activity and histopathological alterations of the S. nonagrioides organs. Venoms were injected into the larvae at five doses (0.15µg, 0.35µg, 0.5µg, 0.75µg and 1µg; fifteen larvae per each dose) and the doses causing death were recorded during the experiment. This study demonstrated that H. saulcyi venom was able to cause external symptoms that eventually led to death of the intoxicated larvae. Moreover, this venom strongly inhibited glutathione S-transferases (GST) activity. Our data highlighted the relationship between the activity of GST enzyme and larval mortalities, so that the inactivation of this enzyme by scorpion venom may be responsible for the increasing the larval mortality. In the present study, the damage of midgut epithelium and malpighian tubules has been directly visualized and documented using histopathology methods. Clear histopathological alterations of intoxication were observed for all larvae injected with 1µg doses of venom, including a distinctive lysing and disorganizing of midgut epithelial cells that coupled with a progressive loss of the peritrophic membrane and the disappearance of microvilli. | ||
| Keywords | ||
| Keywords: Glutathione S-transferases; Hottentotta saulcyi; malpighian tubules; midgut; Sesamia nonagrioides | ||
| References | ||
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