Related Links
Effects of vermicompost and mycorrhizal fungus on quantitative and qualitative traits of medicinal plant Stevia rebaudiana Bertoni | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| Article 11, Volume 35, Issue 3 - Serial Number 95, July and August 2019, Pages 484-500 PDF (319.15 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/ijmapr.2019.123788.2417 | ||
| Authors | ||
| Z. Valinezhad1; A. Gholizadeh* 2; M. Naeemi2; E. Gholamalalipour Alamdari2; M. Zarei2 | ||
| 1M.Sc. student of Agro-ecology, Plant Production Department, Agriculture Science and Natural Resources Faculty, Gonbad Kavous University, Golestan, Iran | ||
| 2Plant Production Department, Agriculture Science and Natural Resources Faculty, Gonbad Kavous University, Golestan, Iran | ||
| Abstract | ||
| In order to investigate the effect of vermicompost and mycorrhizal fertilizers on Stevia rebaudiana Bertoni, a factorial experiment was conducted in a completely randomized design with four replications in the research greenhouse of Agriculture College of Gonbad Kavous University in 2016-2017. The First factor was vermicompost at four levels (0, 10, 20 and 30 % of soil weight) and the second factor was inoculation with biological fertilizers at three levels (no inoculation (control) and inoculation with mycorrhizal fungi Glomus intraradieces and Glomus mosseae about 400 fungi spores per pot). The results showed that all cuttings were gradually eliminated in the treatments containing 30% vermicompost due to its high salinity, and had very low growth in treatments containing 20% vermicompost. The effect of vermicompost and mycorrhizal fungi on all traits studied, except the main effect of mycorrhizal fungi on the number of lateral branches and anthocyanins, were significant at 1% probability level. Also, their interaction was significant for all traits except stem dry weight. Based on the results, the highest positive effect was obtained in application of G. mosseae with 10% vermicompost for morphological traits such as plant height (126.63 cm) and root length (33.75 cm), and G. intraradieces with 10% vermicompost for flower (1.03 g) and root (9.93 g) dry weight and leaf area (78212 cm2) compared to no inoculation of mycorrhizal fungi at different levels of vermicompost. The highest content of chlorophylls a and b and total chlorophyll, and carotenoids (1.25‚ 1.58‚ 2.83 and 1.08 mg gr-1) was obtained in application of G. intraradieces with 10%vermicompost. The maximum content of soluble sugars (158.15 mg gr-1) was observed in 10%vermicompostwithout mycorrhizal inoculation treatment. According to the results of this study, the use of G. mosseae and G. intraradices fungi together with 10% vermicompost had the most positive effect on the quantitative and qualitative characteristics of Stevia rebaudiana. | ||
| Keywords | ||
| Stevia rebaudiana Bertoni‚ inoculation‚ carotenoids‚ vermicompost; Glomus | ||
| References | ||
|
- Ameri, A., tehranifar, A., Davarinezhad, Gh. and Shour, M., 2011. Effect of different substrat on strawberry growth and yield inside in soilless culture. Journal of Small Fruits, 1: 35-50. - Arancon, N., Edwards, C.A., Bierman, P., Welch, C. and Metzger, J.D., 2004. Influences of vermicomposts on field strawberries, 1. effects on growth and yields. Bioresource Technology, 93: 145-53. - Arguello, J.A., Ledesma, A., Nunez, A.S.B., Rodriguez, C.H. and Goldfarb, M.D.D., 2006. Vermicompost affects onbulbing dynamics, nonstructural effects on bulbing dynamics. nonstructural paraguayo garlic bulbs. Horticultural Science, 41: 589-592. - Arnon, A.N., 1967. Method of extraction of chlorophyll in the plants. Agronomy Journal, 23: 112-126. - Baslam, M., Esteban, R., Garcia-Plazaola, J.I. and Goicoechea, N., 2012. Effectiveness of Arbuscular mycorrhizal fungi (AMF) for inducing the accumulation of major carotenoids, chlorophylls and tocopherol in green and red leaf lettuces. Applied Microbiology and Biotechnology, 19: 1-10. - Bigonah, R., Rezvani Moghadam, P. and Jahan, M., 2015. Effect of different fertilizer management on certain quantitative and qualitative properties of medicinal plants Coriandrum sativum L. Iranian Journal of Field Crops Research, 12(4): 574-581. - Bitarafan, N., Gholami, A., Abbas Dokht, H., Baradaran, M. and Khalighi Sigaroodi, F., 2017. Effects of vermicompost and mycorrhizal fungi on growth characteristics, essential oil and yield of thyme (Thymus vulgaris L.). Agroecology Journal, 9(1): 102-114. - Caceres, A., 2000. Calidad de la material prima para la elaboracion de productos fitofarma ceuticas. Primer CongresoInternational FITO 2000 Por la investigacion, conservacion y diffusion del conocimiento de las plantas medicinals. Lima, Peru. 27-30 de septiembre. - Crozier, A., Jaganath, I.B. and Clifford, M.N., 2006. Phenols, polyphenols and tannins: An overview: - Darzi‚ M. T.‚ Hajseyed Hadi‚ M.R. and Rejali‚ F.‚ 2011. Effects of vermicompost and phosphate biofertilizer application on yield and yield components in Anise (Pimpinella anisum L.). Iranian Journal of Medicinal and Aromatic Plants, 26(4): 452-465. - Demir, S., 2004. Influence of arbuscular mycorrhiza on some physiological, growth parameters of pepper. Turkish Journal of Biology, 28: 85-90. - Evelin, H., Kapoor, R. and Giri, B., 2009. Arbuscular mycorrhizal fungi in alleviation of salt stress: A review. Annals of Botany, 104: 1263-1280. - Fallahi, J., 2009. Effects of biofertilizers and chemical fertilizers on quantity and quality characterize of Chamomile (Matricaria Chamomilla L.) as a medicinal plant. M.Sc. Thesis. Faculty of Agriculture. Ferdowsi Universuty of Mashhad, Iran. - Fasihi, M., Shamshiri, M., Karimi, H. and Roosta, H., 2014. Effect of arbuscular mycorrhiza (Glomus mosseae) on growth of greenhouse cucumber (Cucumis sativus cv. Nahid) under different levels of sodium bicarbonate in irrigation waterte. Journal of Science and Technology of Greenhouse Culture, 5(1): 53-62. - Ghasemzadeh, A. and Jaafar, H.Z.E., 2011. Effect of co2 enrichment on synthesis of some primary and secondary metabolites in ginger (Zingiber officinale Roscoe). International Journal of Molecular Sciences, 12: 1101-1114. - Gnanumutry, D.G., 1992. Spacing and nitrogen requirement of sesame. Indian Journal Agronomy, 37: 50-59. - Goldani, M., Kamali, M., Mohtashami, S. and Ghani, A., 2016. Effect of different vermicompost levels on morphophysiological traist and growth charachtristics of basil (Ocimum basilicum L.). Iranian Journal of Soil Research, 30(3): 257-269. - Goussous, S.J. and Mohammad, M.J., 2009. Effect of two arbuscular mycorrhizae and N and P fertilizers on growth and nutrient uptake of onions. International Journal of Agriculture & Biology, 11: 463-467. - Gupta, M.L., Prasad, A., Ram, M. and Kumar, S., 2002. Comparative effects of the vesicular-arbuscular mycorrhizal (VAM) fungus Glomus fasciculatum on the essential oil yield related cultivars of menthol mint (Mentha arvensis) under field conditions. Bioresource Technology, 81: 77-79. - Gutierrez, F.A., Santiago, J., Molina, J.A., Nafate, M., Abud, C.C., Llaven, M.M., Rincon, A.O. and Dendooven, L.R., 2007. Vermicompost as a soil supplement to improve growth, yield and fruit quality of tomato (Lycopersicum esculentum L.). Bioresource Technology, 98: 2781-2786. - Hassan, F.A.S., 2009. Response of Roselle (Hibiscus sabdariffa L.) plant to some biofertilization treatments. Annals of Agriculture Sciences, 54: 437-446. - Hosseini, P., 2007. Physiological study of the effect of cold stress on the seedling stage of different rice genotypes. Ph.D thesis, Shahid Chamran University of Ahvaz, Iran, 145p. - Humphrey, T.V., Richman, A.S., Menassa, R. and Jim, E., 2006. Spatial organisation of four enzymes from Stevia rebaudiana Bertoni that are involved in steviol glycoside synthesis. Plant Molecular Biology, 61: 47-62. - Hwang, S.J., 2006. Rapid in vitro propagation and enhanced stevioside accumulation in Stevia rebaudiana Bertoni. Journal of Plant Biology, 49(4): 267-270. - Inanlo, M., Omidi, H. and Pazoki, A., 2013. The agronomic changes and oil content of purslane (Portulaca oleracea L.) under drought stress and bio-fertilizer / nitrogen chemical. Journal of Medicinal Plants, 48: 170-184. - Jalilian, J. and Heydarzadeh, S., 2016. Effect of cover crops, organic and chemical fertilizer on the quantitative and qualitative characteristics of Safflower (Carthamus tinctorius). Agricultural Science and Sustainable Production, 25(4): 71-85. - Karuppusamy, S., 2009. A review on trends in production of secondary metabolites from higher plants by in vitro tissue, organ and cell cultures. Journal of Medicinal Plants, 3(13): 1222-1239. - Kashefi, B., Booyeh, B. and Alipour, Z., 2015. Essential oils, organic agriculture, organic fertilizers, zuleng. Journal of Plant Process and Function, 4(13): 145-151. - Kochert, G., 1978. Carbohydrate determination by the phenol sulfuric acid method: 96-97. In: Helebust, J.A. and Craigie, J.S., (Eds.). Hand Book of Physiological Methods (Vol. 2). Physiological and Biochemical Methods. Cambridge University Press, Cambridge, 512p. - Koozehgar Kaleji, M., 2014. Qualitative and quantitative response of Eryngium caeruleum to mycorrhizal symbiosis, azotobacter and various levels of plant densities. Thesis M.Sc. in Agro-ecology, Islamic Azad University Karaj Branch. - Lattanzio, V., Cardinali, A., Ruta, C., Fortunato, I.M., Lattanzio, V.M.T. and Linsalata, V., 2009. Relationship of secondary metabolism to growth in oregano (Origanum vulgare L.) shoot cultures under nutritional stress. Environmental and Experimental Botany, 65: 54-62. - Mafakheri, S., Omidbaigi, R., Sefidkon, F. and Rejali, F., 2012. Effect of vermicompost, bio phosphate and azotobacter on quantity and quality of essential oil of Dracocephalum moldavica L. Iranian Journal of Medicinal and Aromatic Plants, 27(4): 596-605. - Mandal, S., Evelin, H., Giri, B., Singh, V. and Kapoor, R., 2013. Arbuscular mycorrhiza enhances the production of stevioside and rebaudioside-A in Stevia rebaudiana via nutritional and non-nutritional mechanisms. Applied Soil Ecology, 72: 187-194. - Mardani, F. and Amooaghaie, R., 2016. Effect of vermicompost and its extract on emergence and the growth parameters of Plantago psyllium. Journal of Science and Technology of Greenhouse Culture, 7(1): 1-13. - Martinov, M., Oztekin, S. and Muller, J., 2007. Drying: 85-97. In: Oztekin, S. and Martinov, M. (Eds.). Medicinal and Aromatic Crops: Harvesting, Drying, and Processing. CRC Press, United States of America, 320p. - Moghadam, A.R., Oraghi, Z. and Saidi, F., 2012. Vermicompost induced changes in growth and development of Lilium asiatic hybrid var. Navona. Agriculture Reseach, 7(17): 2609-2621. - Mohammadi, M., Tobeh, A., Vahidipour, H.R. and Fakhari, R., 2013. Effects of biological fertilizers on essential oil components and quantitative and qualitative yield of lemon verbena (Lippia citriodora). International Journal Agriculture Crop Science, 5(12): 1374-1380. - Naghdi Badi, H., Lotfizad, M., Qavami, N., Mehrafarin, A. and Khavazi, K., 2013. Response of quantity and quality yield of Valerian (Valeriana officinalis L.) to application of phosphorous Bio/Chemical fertilizers. Journal of Medicinal Plants, 2(46): 25-37. - Norzad, S., Ahmadian, A., Moghaddam, M. and Daneshfar, E., 2014. Effect of drought stress on yield, yield components and essential oil in coriander treated with organic and inorganic fertilizers. Agriculture Crop Management, 16(2): 289-302. - Phuong, M., Nguyen, E.M. and Niemeyer K.E.D., 2010. Potassium rate alters the antioxidant capacityand phenolic concentration of basil (Ocimum basilicum L.) leaves. Food Chemistry, 123: 1235-1241. - Ravikumar, S., Kathiresan, K., Thadedus, M., Ignatiammal, S., Babu Selvam, M. and Shanthy, S., 2004. Nitrogenfixing azotobacters from mangrove habitat and their utility as marine biofertilizers. Journal of Experimental Marine Biology and Ecology, 312: 5-17. - Razavi Nia, S.M., Aghaalikhani, M. and Naghdi Badi, H.A., 2015. Effect of vermicompost and chemical fertilizers on quantitative and qualitative properties of Echinaceae pursuerea (L.) Moench. Iranian Journal Medicinal and Aromatic Plants, 31(2): 357-373. - Rezvani, M. and Ardakani, M.R., 2009. Interaction between alfalfa (Medicago sativa L.) mycorrhizal roots traits and heavy metal (Cd, Co, pb). International Symposium "Root Research and Applications" Root RAP, 2-4 September, Boku-Vienna, Austria. - Sairkar, P., Chandravanshi, M.K., Shukla, N.P. and Mehrotra, N.N., 2009. Mass production of an economically important medicinal plant Stevia rebaudiana using in vitro propagation techniques. Journal of Medicinal Plants, 3(4): 266-270. - Saydi, Z., Fateh, E. and Aynehband, A., 2017. Effect of different sources of nitrogen and organic fertilizers on yield and yield components of Ajowan (Trachyspermum ammi L.). Journal of Agroecology, 9(1): 115-128. - Sedghi Moghadam, M. and Mirzaei, M., 2008. The effect of urban waste compost on the quantitative and qualitative characteristics of pumpkin. 3rd National Congress of Recycling and Reuse of Orgaic Renewable Resources in Agriculture. Islamic Azad University, Isfahan (Khorasgan) Branch, Iran, 13 May: 1-7. - Selvaraj, T. and Chellappan, P., 2006. Arbuscular mycorrhizae: Adiverse personality. Review Paper. Central Europen Agriculture Journal, 7: 349-358. - Shirzadi, F., Ardakani, M. and Asadi Rahmani, H., 2014. Effect of biofertilizers on quantitative characterization of basil (Ocimum basilicum L.). Agroecology Journal, 6(3): 542-551. - Sing, R., Sharma, R.R., Kumar, S., Gupta, R.K. and Patil, R.T., 2008. Vermicompost substitution influences growth, physiological disorders, fruit yield and quality of strawberry (Fragaria xananssa Duch.). Bioresource Technology, 99: 8507-8511. - Tadayon, M.R. and Zarei‚ M., 2014. The effect of the arbuscular mycorrhizal (AM) fungus Glomus mosseae on the growth and yield of of three ecotype of hemp (Cannabis sativa L.) under saline soil and saline water. Journal of Plant Process and Function, 3(7): 105-114. - Vitrac, X., Larronde, F., Krisa, S., Decendit, A., Deffieux, G. and Mérillon, J.M., 2000. Sugar sensing and Ca2+ calmodulin requirement in Vitis vinifera cells producing anthocyanins. Phytochemistry, 53: 659-665. - Wagner, G.J., 1979. Content and vacuole/extra vacuole distribution of neutralsugars, free amino acids and anthocyanins in protoplasts. Plant Physioology, 64(4): 88-93. - Weaam, R.A., Sakr, H.M., Elbagoury, M., Sidky, A. and Ali, S.A., 2014. Production of organic roselle by natural minerals and biofertilizers. American-Eurasian Journal of Agricultural & Environmental Sciences, 14(10): 985-995. - Yavary, Z., Moradi, H., Barzegar Golchini, B. and Sadeghy, H., 2015. Evaluation of Aloe vera (Aloe barbadensis Miller) antioxidant activity and some of morphological characteristics in different of vermicompost and nano-potassiom particles amounts. Journal of Plant Process and Function, 4(12): 95-104. - Yosefi Shiadeh, S., Chalavi, V. and Zangi, S., 2015. The effect of different levels of vermicompost and photoperiod on greenhouse production of medicinal plant stevia (Stevia rebaudiana Bertoni). Journal of Science and Technology of Greenhouse Culture, 6(1): 31-39. - Zare Hoseini, R., Mohammadi Goltapeh, E., Kalatejari, S. and Dehghani Mashkani, M., 2015. Effect of vermicompost and fungi inoculation on growth characteristics and steviosid content of Stevia rebaudiana Bert. Journal of Medicinal Plants, 4(56): 179-188. | ||
|
Statistics Article View: 865 PDF Download: 508 |
||
Journal management system. designed by sinaweb