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Rafei, N., Namavari, M. (2019). Study of antiparasitic effects of Ginger and Glycyrrhizin aqua extracts against Neospora caninum. , 32(3), 47-54. doi: 10.22092/vj.2019.123760.1511
Nasrin Rafei; Mehdi Namavari. "Study of antiparasitic effects of Ginger and Glycyrrhizin aqua extracts against Neospora caninum". , 32, 3, 2019, 47-54. doi: 10.22092/vj.2019.123760.1511
Rafei, N., Namavari, M. (2019). 'Study of antiparasitic effects of Ginger and Glycyrrhizin aqua extracts against Neospora caninum', , 32(3), pp. 47-54. doi: 10.22092/vj.2019.123760.1511
Rafei, N., Namavari, M. Study of antiparasitic effects of Ginger and Glycyrrhizin aqua extracts against Neospora caninum. , 2019; 32(3): 47-54. doi: 10.22092/vj.2019.123760.1511

Study of antiparasitic effects of Ginger and Glycyrrhizin aqua extracts against Neospora caninum

تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
Article 6, Volume 32, Issue 3, September 2019, Pages 47-54    PDF (575.51 K)
Document Type: Full Research Paper
DOI: 10.22092/vj.2019.123760.1511
Authors
Nasrin Rafei1; Mehdi Namavari* 2
1Group Bioshimi, Islamic Azad University Branch Shiraz
2Shiraz Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, Iran
Abstract
Neospora caninum, the cause of Neosporosis, is an obligate intracellular parasite. Neosporosis infection has worldwide distribution and infect many animals such as cattle, sheep, goat and deer. The control methods of disease are included test and cull, antibiotic treatment and vaccination. The lack of proper drugs and the absence of effective vaccines led us to evaluate the anti neospora effects of Ginger and Glycyrrhizin aqua extracts. In this study, different doses of two herbal extracts were prepared. At first the cytotoxicity of aqua extracts was evaluated on Vero cell line by MTT assay. Then their effects on parasite were tested in chicken embryonated eggs. The results showed that Ginger and Glycyrrhizin aqua extract, have no cytotoxic effect on Vero cell line in 0.625 mg/ml dose. Also, in the study on embryonic eggs, there were casualties in the groups that used clindamycin and Glycyrrhizin,while the ginger prevents losses due to the neosporosis in chicken embryonated eggs. Previous reports had proved the effect of ginger on Toxoplasma gondii .In this study, the inhibitory effect of this plant was also shown on Neospora caninum. In addition, for the first time embryonated eggs were used to evaluate drug effects on Neospora caninum. Both of these findings can be useful in applied research for the preparation of an effective drug for the control and treatment of neosporosis.
Keywords
Neospora caninum; MTT; Aqua extract; Ginger; Glycyrrhizin; Chicken embryonated egg
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