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Ataei Kachooei, S., motamed, N., Ataei Kachooei, S. (2019). Cross-reactive immunogenic proteins of P.multocida serotypes: candidates for improving vaccine. , 32(3), 38-46. doi: 10.22092/vj.2019.116524.1396
Saeed Ataei Kachooei; najmeh motamed; Saba Ataei Kachooei. "Cross-reactive immunogenic proteins of P.multocida serotypes: candidates for improving vaccine". , 32, 3, 2019, 38-46. doi: 10.22092/vj.2019.116524.1396
Ataei Kachooei, S., motamed, N., Ataei Kachooei, S. (2019). 'Cross-reactive immunogenic proteins of P.multocida serotypes: candidates for improving vaccine', , 32(3), pp. 38-46. doi: 10.22092/vj.2019.116524.1396
Ataei Kachooei, S., motamed, N., Ataei Kachooei, S. Cross-reactive immunogenic proteins of P.multocida serotypes: candidates for improving vaccine. , 2019; 32(3): 38-46. doi: 10.22092/vj.2019.116524.1396

Cross-reactive immunogenic proteins of P.multocida serotypes: candidates for improving vaccine

تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
Article 5, Volume 32, Issue 3, September 2019, Pages 38-46    PDF (574.01 K)
Document Type: Full Research Paper
DOI: 10.22092/vj.2019.116524.1396
Authors
Saeed Ataei Kachooei* 1; najmeh motamed1; Saba Ataei Kachooei2
1Department of Avian Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran
2Department of Cellular Molecular Biology, Faculty of Life Sciences, Kharazmi University, Karaj, Iran
Abstract
Because P.multocida serotypes have no cross protection Several studies have been focused on identification of immunogenic molecules shared between different serotypes in the hope of production of a wide-range vaccine effective against all causative serotypes. In the present study, crude sonicated cell extracts of P. multocida serotypes A:1, A:3, and A:4 were immunoblotted against chicken hyper-immune sera in order to identify cross-reactive immunogenic proteins of them. 35 chickens aged two months randomly divided into 7 groups of five and were injected by monovalent or trivalent serotypes with time intervals of 14 days between first and second injections, and 10 days between second and third and third to fourth injections. The first inoculum was adjuvanted with complete Freund adjuvant (CFA), the second and third inocula were adjuvanted with incomplete Freund adjuvant (IFA), and the fourth one was without any adjuvant. Results showed that there are at least 5 cross-reactive immunogenic proteins in serotypes A:1, A:3, and A:4, in a way that antibodies produced by chicken in an immune response to immunization with whole cell Pasteurella suspensions are able to react with each of the 3 serotypes. These cross-reactive agents are proteins (or at least in some parts) with the molecular weight of 39, 40, 37, 53, and 30 K.Da. The 39 K.Da band was the most prominent immunoblott and introduced as candidate for molecular identification and future vaccine studies.
Keywords
Pasteurella multocida; fowl cholera; cross-reactive immunogen, vaccine
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