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واکنش فلورسانس کلروفیل در ارقام گلابیِ حساس و متحمل به بیماری آتشک در شرایط کلروپلاستی فعال و غیرفعال | ||
| به زراعی نهال و بذر | ||
| مقاله 5، دوره 34، شماره 1، خرداد 1397، صفحه 73-87 اصل مقاله (260.08 K) | ||
| شناسه دیجیتال (DOI): 10.22092/sppj.2018.118102 | ||
| نویسندگان | ||
| زینب صالحی1؛ حمید عبداللهی* 2؛ سیدمهدی میری1 | ||
| 1گروه علوم باغبانی، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران | ||
| 2پژوهشکده میوههای معتدله و سردسیری، مؤسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران | ||
| چکیده | ||
| بیماری آتشک در اثر متقابل با درختان میزبان، خسارت قابلتوجهی را از طریق انفجار اکسیداتیو و در نهایت نکروز بافتها ایجاد میکند. در این تحقیق بهمنظور بررسی نقش زنجیره انتقال الکترون کلروپلاستی در این اثر متقابل، به ارزیابی واکنش فلورسانس کلروفیل در ارقام گلابی حساس و متحمل به بیماری در شرایط کلروپلاستهای فعال و غیرفعال پرداخته شد. بهمنظور مقایسه کلروپلاستهای فعال و غیرفعال، آزمایشها در شرایط درون شیشه در حضور سوکروز (زنجیره الکترونی کلروپلاستی غیرفعال) و عدم حضور سوکروز (زنجیره الکترونی کلروپلاستی فعال) انجام شد. نتایج نشان داد پیشرفت بیماری در رقم حساستر و همچنین در حضور سوکروز سریعتر بود. همچنین شاخصهای فلورسانس کلروفیل حداقل (Fo) و حداکثر (Fm) در حضور سوکروز در مقایسه با شرایط عدم حضور سوکروز کاهش یافت؛ بنابراین نقش منفی حضور سوکروز در فعالیت کلروپلاستها تأیید شد. مقایسه تأثیر حمله پاتوژن بر شاخصهای فلورسانس کلروفیل حداقل و حداکثر در شرایط حضور و عدم حضور سوکروز نشانگر تأثیر حداقلی حمله پاتوژن بر شاخص حداقل فلورسانس کلروفیل در حضور سوکروز بود، در حالی که در رقم حساس و در شرایط عدم حضور سوکروز این شاخص تغییرات زیادی را نشان داد. | ||
| کلیدواژهها | ||
| گلابی؛ Erwinia amylovora؛ نکروز؛ زنجیره انتقال الکترون؛ سوکروز | ||
| عنوان مقاله [English] | ||
| Chlorophyll Fluorescence Response in Susceptible and Tolerant Pear Cultivars to Fire Blight in Active and Inactive Chloroplast Conditions | ||
| نویسندگان [English] | ||
| Z. Salehi1؛ H. Abdollahi2؛ S. M. Miri31 | ||
| 1Department of Horticultural Science, Karaj Branch, Islamic Azad University, Karaj, Iran | ||
| 2Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| چکیده [English] | ||
| Fire blight disease in interaction with host trees causes considerable damages via oxidative burst and finally necrosis on the tissues. In this research, to investigate the role of chloroplast electron transfer chain (ETC) in this interaction, the chlorophyll fluorescence response of susceptible and tolerant in vitro pear cultivars were studied in both active and inactive chloroplast conditions. For comparison of active and inactive ETC of chloroplasts, the experiments were conducted in the sucrose presence (inactive ETC) and absence (active ETC) in the growth media. The results showed faster disease progression in susceptible cultivar, as well as in sucrose presence condition. Also, the minimum (Fo) and maximum (Fm) chlorophyll fluorescence indices were reduced in sucrose presence compared to the sucrose absence condition; Therefore, the negative role of sucrose presence in the activity of chloroplasts was confirmed. Comparing the effects of pathogen attack on Fo and Fm indices in sucrose presence and absence condition showed minimum effects of pathogen attack on Fo index in the sucrose presence condition, while this index demonstrated high variation in susceptible cultivar in sucrose absence condition. | ||
| کلیدواژهها [English] | ||
| Pear, Erwinia amylovora, necrosis, electron transport chain, sucrose | ||
| مراجع | ||
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