Mehrbanpour, M., Shirazi nejad, A., Hosseini, S. (2019). Effects of various adjuvants on efficacy of experimental inactivated oil Ornithobacterium rhinotracheale vaccine in SPF chickens. , 32(1), 24-30. doi: 10.22092/vj.2018.121238.1445
M.J. Mehrbanpour; Ali Shirazi nejad; S.M.H. Hosseini. "Effects of various adjuvants on efficacy of experimental inactivated oil Ornithobacterium rhinotracheale vaccine in SPF chickens". , 32, 1, 2019, 24-30. doi: 10.22092/vj.2018.121238.1445
Mehrbanpour, M., Shirazi nejad, A., Hosseini, S. (2019). 'Effects of various adjuvants on efficacy of experimental inactivated oil Ornithobacterium rhinotracheale vaccine in SPF chickens', , 32(1), pp. 24-30. doi: 10.22092/vj.2018.121238.1445
Mehrbanpour, M., Shirazi nejad, A., Hosseini, S. Effects of various adjuvants on efficacy of experimental inactivated oil Ornithobacterium rhinotracheale vaccine in SPF chickens. , 2019; 32(1): 24-30. doi: 10.22092/vj.2018.121238.1445

Effects of various adjuvants on efficacy of experimental inactivated oil Ornithobacterium rhinotracheale vaccine in SPF chickens

تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
Article 3, Volume 32, Issue 1 - Serial Number 122, January 2019, Pages 24-30    PDF (716.21 K)
Document Type: Full Research Paper
DOI: 10.22092/vj.2018.121238.1445
Authors
M.J. Mehrbanpour* 1; Ali Shirazi nejad2; S.M.H. Hosseini3
1Assistant Professor, Department of Research and Production of Poultry Inactivated Viral Vaccine, Shiraz Branch of Razi Vaccine and Serum Research institute, Agricultural Research Education and Extension Organization (AREEO).
2Department of Research and Production of Poultry Inactivated Viral Vaccine, Shiraz Branch of Razi Vaccine and Serum Research institute, Agricultural Research Education and Extension Organization (AREEO)
3Assistant Professor, Department of Immunology, Shiraz Branch of Razi Vaccine and Serum Research institute, Agricultural Research Education and Extension Organization (AREEO), Shiraz, Iran.
Abstract
The purpose of this study was the effect of vaccination with different adjuvant of inactivated ORT vaccines (potassium alum, mineral oil and Saponin) to measure the levels of antibodies against ORT in blood sera on SPF chickens. Inactivated ORT bacterin vaccines were prepared from ORT serotype A using potassium alum, mineral oil (MO) and Saponin. Fifteen SPF chickens per group were vaccinated at an age of 3 weeks with one of the three different inactivated vaccines. Similarly, 15 SPF chickens at an age of three week were vaccinated with inactivated vaccine in mineral oil. Booster vaccination with inactivated vaccine in mineral oil was performed five weeks after primary vaccination. Blood samples were collected from all the experimental birds during the experimental study. The presence of IgG antibodies against ORT antigens in SPF chickens were measured by using a modified ELISA, which were prepared in our laboratory. In this study, the levels of antibodies to ORT in blood sera of vaccinated chickens were significantly determined higher by ELISA than non-vaccinated chickens. These results show that the levels of antibodies of ORT vaccine with Montanide adjuvant were significantly greater than that of other vaccines when humoral responses of all vaccine were compared. According to the results, twice vaccination interval of 5 weeks could be effective for long lasting term than a single administration. In conclusion, vaccination of SPF chickens at 3 weeks of age with a booster dose at 8 weeks of age can be an effective method against ORT in chickens.
Keywords
Ornithobacterium rhinotracuim; Inactivate vaccine; Chicken
References

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