Identification of miRNAs and their target genes in red clover (Trifolium pretense) | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| Article 2, Volume 26, Issue 2 - Serial Number 52, December 2018, Pages 156-164 PDF (249.92 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/ijrfpbgr.2018.117954 | ||
| Authors | ||
| M. R. Naghavi* 1; A. A. Karimi2 | ||
| 1Corresponding author, Prof., College of Agriculture and Natural Resources, University of Tehran, Karaj, I.R.Iran Email: mnaghavi@ut.ac.ir | ||
| 2M.Sc. student, College of Agriculture and Natural Resources, University of Tehran, Karaj, I.R.Iran | ||
| Abstract | ||
| DOR: 98.1000/1735-0891.1397.2.155.52.26.1588.41 MicroRNAs (miRNAs) are a group of 18_22 nucleotides long noncoding small endogenous that derived from its precursor sequence and evolutionary conserved post-transcriptional regulatory RNAs, which show an enormous role in various biological and metabolic processes in both animals and plants. MiRNA detection methods including microarray, qRT-PCR , northern blot and bioinformatics methods in which the easiest and cheapest way to identify miRNA is bioinformatics method. A bioinformatics approach was used to identify potential miRNA in red clover. EST-based homology search was applied to find potential miRNA of red clover. We blasted publicly available EST sequences obtained from NCBI GenBank against previously known plant miRNAs. A total of six miRNA target genes based on their complementary sequences were also detected. Target genes encoding mono dehydro ascorbate reductase 1 play an important role in maintaining balance in the cycle of reactive oxygen species ascorbate-glutathione, FES1 proteins that contain zinc finger domain binding DNA, RHF2A gene encoding E3 ubiquitin-protein ligase involved in the positive regulation of the gametogenesis progression, RNA helicase ATP-dependent is involved in the RNA repair and metabolism, Major facilitator superfamily (MFS) is a superfamily of membrane transport proteins that facilitate movement of small solutes across cell membranes in response to chemiosmotic gradients, AT-hook DNA binding motif protein. | ||
| Keywords | ||
| Bioinformatics; EST; homology search; miRNA; target genes | ||
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